Catalog ID
Enhance post-transfection growth and protein production of multiple HEk293 cell lines
Details
BalanCD HEK293 Feed is formulated to significantly increase protein yields in suspension-adapted HEK293 cell lines post-transfection.
- Increase viable cell density and culture duration to maximize yield
- Use the same Feed for a wide range of HEK293 cell lines
- Fulfill regulatory requirements
- Chemically-defined, animal component-free formula
- Drug Master File (DMF) submitted to US FDA
Two-fold increase in titer compared to other commercially-available media
HEK293-6E cells were cultured in duplicate 125mL Erlenmeyer flasks with a 30 mL working volume. Cells were seeded at 3 x 105 cells/mL in BalanCD HEK 293 medium. PEI-mediated transfection was performed on day 3 to produce a biosimilar antibody. BalanCD HEK293 medium was supplemented with a 5% v/v addition of BalanCD HEK293 Feed on days 1-4 post-transfection.? Protein titer was measured on days 7, 9, and 11 post-transfection. Cultures terminated when viability dropped below 70%, (data not shown). Data shown represents the average of two duplicate cultures.
上海金畔生物代理FUJIFILM Irvine Scientific富士膠片歐文科技細胞培養基，部分現貨，量多優惠，歡迎來電咨詢18301939375！
FUJIFILM Irvine Scientific富士膠片歐文科技特色產品
BalanCD™ CHO Platform/培養基平臺
BalanCD™ HEK293 培養基平臺
PRIME-XV免疫細胞培養基
PRIME-XV干細胞培養基
凍存液和其它
免責聲明
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