分類目錄歸檔：Cygnus試劑盒

Cygnus F030 BSA 殘留檢測試劑盒 BSA ELISA Kit (F030)

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Cygnus F030 BSA 殘留檢測試劑盒 BSA ELISA Kit (F030)

Cygnus試劑盒Cygnus technologies熱銷產品

Cygnus熱銷產品：cygnus f975、cygnus f410檢測試劑盒、cygnus f550

F015 CHO宿主蛋白殘留檢測試劑盒
F030 BSA殘留檢測試劑盒
F400 Protein A 試劑盒
F410 大腸桿菌宿主蛋白殘留檢測試劑盒
CM015 CHO宿主蛋白殘留檢測試劑盒

F140 酵母菌宿主蛋白殘留檢測試劑盒
F150 HEK293宿主蛋白殘留檢測試劑盒
F050 蛋白A檢測試劑盒
F500 Vero cell HCP ELISA試劑盒

品牌商名稱：cygnus
公司官網：

Cygnus F030 BSA 殘留檢測試劑盒 BSA ELISA Kit (F030)

Product Information
Product Type: ELISA Kit
Storage: All kit reagents 2°C to 8°C
Assay format: 96 Well Plate
Time to result: ~1 hr. 50 min
LOD: ~125 pg/mL
LLOQ: ~250 pg/mL
Recommended Diluent: F031A

Description

This kit is intended for quantitating bovine serum albumin (BSA). The use of serum-free defined media greatly reduces the number of potential impurities, but it may still be necessary to determine the levels of trace impurities, such as proteins and growth factors used in these media. Most commercial formulations of serum-free media contain significant amounts of albumin and transferrin, either of bovine or human origin, and insulin from various species.

This highly sensitive assay for BSA has a detection limit of <250 pg/ml. It can be used to optimize purification processes and in routine quality control of in-process streams, as well as in testing the final product. Please use?Sample Diluent, Item No. F031A, with these kits; available separately.

描述
該試劑盒用于定量牛血清白蛋白 (BSA)。 使用無血清成分的培養基大大減少了潛在雜質的數量，但仍可能需要確定微量雜質的水平，例如這些培養基中使用的蛋白質和生長因子。 大多數商業化的無血清培養基配方含有大量的白蛋白和轉鐵蛋白，無論是來自牛的還是來自人的，以及來自不同物種的胰島素。

這種高靈敏度的 BSA 檢測方法的檢測限 <250 pg/ml。 它可用于優化純化過程和過程中流的常規質量控制，以及測試最終產品。 請在這些套件中使用樣品稀釋劑，貨號 F031A； 可單獨購買。

Kit Components
Anti-BSA:HRP Conjugate
BSA Standards Set, A-E
TBS Wash Concentrate, 20X
Stop Solution
TMB Substrate for ELISA
Anti-BSA Coated Microtiter Strips

Cygnus其他相關試劑盒：

Cygnus? ?I028 Sample Diluent Buffer
Cygnus? ?F220 NS/0 HCP ELISA 試劑盒
Cygnus? ?F050h Protein A-h ELISA 試劑盒
Cygnus?? F015???? CHO HCP ELISA Kit 試劑盒????????????? 1 Kit
Cygnus?? F550???? CHO HCP ELISA Kit, 3G 試劑盒?????????? 1 Kit
Cygnus?? CM015??? CHO-CM HCP ELISA Kit 試劑盒???????????????? 1 Kit
Cygnus?? F410???? E.coli HCP ELISA Kit 試劑盒??????????????? 1 Kit
Cygnus?? F500???? Vero Cell HCP ELISA Kit 試劑盒?????? 1 Kit
Cygnus?? F510???? BHK HCP ELISA Kit 試劑盒????????????? 1 Kit
Cygnus?? F135???? S.cerevisiae HCP ELISA Kit????????????? 1 Kit
Cygnus?? F020???? SF9 HCP ELISA Kit 試劑盒??????????????? 1 Kit
Cygnus?? F650???? HEK 293 HCP ELISA Kit 試劑盒?????????????? 1 Kit
Cygnus?? F490???? L.lactis HCP ELISA Kit 試劑盒?????????? 1 Kit
Cygnus?? F300???? MRC5 HCP ELISA Kit 試劑盒???????????????????? 1 Kit
Cygnus?? F650???? HEK 293 HCP ELISA Kit 試劑盒???????? 1 Kit
Cygnus?? F230???? A549 HCP ELISA Kit 試劑盒??????????? 1 Kit
Cygnus?? F220???? NS/0 HCP ELISA Kit 試劑盒??????????????? 1 Kit
Cygnus?? F450???? P.fluorescens HCP ELISA Kit?????????????? 1 Kit
Cygnus?? F530???? PER.C6 HCP ELISA Kit 試劑盒???????????? 1 Kit
Cygnus?? F140???? Pichia pastoris HCP ELISA Kit????????? 1 Kit
Cygnus?? F240???? Total Goat Milk Proteins ELISA Kit????? 1 Kit
Cygnus?? F320???? S.aureus HCP ELISA Kit????????????????? 1 Kit
Cygnus?? F180???? SP2/0 HCP ELISA Kit 試劑盒??????????????? 1 Kit
Cygnus?? F500???? Vero Cell HCP ELISA Kit 試劑盒????????? 1 Kit
Cygnus?? F120???? Bovine Transferrin ELISA Kit 試劑盒??????? 1 Kit
Cygnus?? F030???? BSA ELISA Kit 試劑盒?????????????????????? 1 Kit
Cygnus?? F280???? Insulin ELISA Kit, Ultra-Sensitive????????? 1 Kit
Cygnus?? F045???? Mouse IgG1 ELISA Kit 試劑盒??????????????? 1 Kit
Cygnus?? F210???? Goat IgG ELISA Kit 試劑盒????????????????? 1 Kit
Cygnus?? F165???? Human IgA ELISA Kit 試劑盒???????????????? 1 Kit
Cygnus?? F070???? Bovine IgG ELISA Kit 試劑盒??????????????? 1 Kit
Cygnus?? F290???? Bovine Plasma Proteins ELISA Kit 試劑盒??? 1 Kit
Cygnus?? F160???? Human IgG, Total, ELISA Kit 試劑盒???????? 1 Kit
Cygnus?? F170???? Human IgM ELISA Kit 試劑盒???????????????? 1 Kit
Cygnus?? F055???? Human Serum Albumin ELISA Kit 試劑盒??????? 1 Kit
Cygnus?? F035N??? Human Transferrin ELISA Kit 試劑盒????????? 1 Kit
Cygnus?? F050???? Protein A ELISA Kit 試劑盒???????????????? 1 Kit
Cygnus?? F050H???? Protein A-h ELISA Kit (human IgG)????????? 1 Kit
Cygnus?? F040???? Insulin ELISA Kit 試劑盒?????????????????? 1 Kit
Cygnus?? F046???? Mouse IgG2a ELISA Kit 試劑盒?????????????? 1 Kit
Cygnus?? F047???? Mouse IgG2b ELISA Kit 試劑盒?????????????? 1 Kit
Cygnus?? F090???? Mouse IgM ELISA Kit 試劑盒???????????????? 1 Kit
Cygnus?? F049???? Mouse Total IgG ELISA Kit 試劑盒?????????? 1 Kit
Cygnus?? F400???? Protein A ELISA Kit 試劑盒???????????????? 1 Kit

Cygnus Technologies CM015 CHO-CM HCP ELISA 試劑盒

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Cygnus Technologies CM015 CHO-CM HCP ELISA 試劑盒

Cygnus試劑盒Cygnus technologies熱銷產品

Cygnus熱銷產品：cygnus f975、cygnus f410檢測試劑盒、cygnus f550

品牌商名稱：cygnus
公司官網：

Cygnus technologies, Inc. （天鵝公司） 是美國一家專門從事生物工程和制藥過程中殘留物檢測的一系列產品。生物制品生產過程中存在許多影響產品質量的殘留物，諸如特殊重組表達系統中宿主細胞蛋白（ Host Cell rotein, HCP ）的殘留以及生物制品加工過程中培養物殘留的污染物。塞納斯公司提供了廣泛而靈敏度極高的一系列用于檢測生物工程殘留物的產品，在尋求最佳純化效果和日常質量控制過程中使用塞納斯的產品可以方便地得到理想的實驗結果。

產品主要包括以下幾類：
ELISA 試劑盒－ 檢測宿主細胞蛋白殘留和生物制品生產過程污染物。

Western Blot 試劑盒－ 檢測并鑒定常用重組表達系統中的宿主細胞蛋白。

免疫學檢測試劑－ 為生物醫藥行業提供檢測生物制品免疫原性的方法。

抗體－ HCP 抗體和檢測微生物用抗體。

緩沖液－ 樣品稀釋緩沖液和酶標抗體稀釋緩沖液

F015 CHO 宿主蛋白殘留檢測試劑盒

F030 BSA 殘留檢測試劑盒

F410 大腸桿菌宿主蛋白殘留檢測試劑盒

CM015 CHO 宿主蛋白殘留檢測試劑盒

F140 酵母菌宿主蛋白殘留檢測試劑盒

F150 HEK293 宿主蛋白殘留檢測試劑盒

F050 蛋白 A 檢測試劑盒
Cygnus Technologies, Inc. provides the Biotechnology and Biopharmaceutical industry with products and analytical methods, designed to accelerate the R&D phase, improve process development and reduce the costs associated with labor intensive quality control procedures. We offer a comprehensive menu of well validated convenient to use microtiter plate ELISA methods to detect Host Cell Proteins from specific recombinant expression systems and bioprocess contaminants used in culture media as well as transgenic expression systems in goats and cattle. These simple to use kits are highly sensitive and are intended to aid in optimal purifications process development and routine quality control.

CHO-CM HCP ELISA Kit (CM015)

Product Information
Product Type: ELISA Kit
Storage: All kit reagents 2°C to 8°C
Target Expression System: CHO
Species Group: Mammalian
Species: Chinese hamster
Assay format: 96 Well Plate
Time to result: ~3 hrs. 50 min
LOD: 100 pg/mL
LLOQ: ~0.7 ng/mL
Recommended Diluent: I028

Description

This assay is intended for use with pharmaceutical products secreted into protein-free CHO growth media. The antibody is raised and affinity-purified against, then calibrated with HCPs typically found in CHO protein-free conditioned media. Special procedures were utilized in the generation of these antibodies to ensure that low molecular weight and less immunogenic impurities, as well as high molecular weight components, would be represented. Our assay qualification has determined that the LOD for this kit is <200 pg/ml. Please use?Sample Diluent, Item No. I028, with these kits; available separately.

描述
該測定旨在用于分泌到無蛋白質 CHO 生長培養基中的藥物產品。 產生抗體并對其進行親和純化，然后用通常在無 CHO 蛋白條件培養基中發現的 HCP 進行校準。 在產生這些抗體時使用了特殊程序，以確保代表低分子量和較少免疫原性雜質以及高分子量成分。 我們的檢測鑒定已確定該試劑盒的 LOD <200 pg/ml。 請在這些套件中使用樣品稀釋劑，貨號 I028； 可單獨購買。

Kit Components
Anti-CHO-CM:HRP Conjugate
CHO-CM HCP Standards Set, A-G
Stop Solution
TBS Wash Concentrate, 20X
TMB Substrate for ELISA
Anti-CHO Coated Microtiter Strips

Cygnus Vero Cell HCP ELISA Kit (F500)試劑盒

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Cygnus Vero Cell HCP ELISA Kit (F500)試劑盒

Cygnus試劑盒Cygnus technologies熱銷產品

Cygnus熱銷產品：cygnus f975、cygnus f410檢測試劑盒、cygnus f550

品牌商名稱：cygnus
公司官網：

Cygnus Technologies, Inc.美國賽納斯科技公司簡介

This product has been depleted and replaced with Vero Cell 2G HCP ELISA Kit (F975).

We have resupplied F500 due to its capture and detection antibody stock depletion.

Cygnus Technologies, Inc.(美國賽納斯科技公司)是一家專為疫苗生產企業、基因工程藥企、研發部門及QA部門(品質保證部門)提供宿主細胞蛋白殘留檢測和生物工藝污染物檢測技術和產品的世界*的美國公司。包括美國和中國在內的全球生物制藥企業,生物制品生產過程中存在許多影響產品質量的殘留物,諸如特殊重組表達系統中宿主細胞蛋白(Host Cell Protein,HCP)的殘留以及生物制品加工過程中培養物殘留的污染物(Bioprocess Contaminant, BC)。美國Cygnus公司提供了種類廣泛而靈敏度極高的一系列用于檢測生物工程殘留物的HCP ELISA試劑盒和BC ELISA試劑盒等產品,在尋求*純化效果和日常質量控制過程中使用美國Cygnus公司的產品可以很方便地得到理想的實驗結果。

美國Cygnus公司提供的ELISA試劑盒產品主要包括以下兩類:

Host Cell Protein Analysis ELISA Kits(宿主細胞蛋白分析ELISA試劑盒)

其來源種屬有:哺乳動物細胞、細菌、酵母、昆蟲細胞、轉基因細胞。

Cygnus Vero Cell HCP ELISA Kit (F500)

Product Information
Product Type: ELISA Kit
Storage: All kit reagents 2°C to 8°C
Target Expression System: Vero Cell
Species Group: Mammalian
Species: African green monkey

Description

Important Change Notification Memo: Vero Cell HCP ELISA Kit

New Vero Cell 2G HCP ELISA Kit, Item Number F975 now available

Expression of viral vaccines and other therapeutic proteins in Vero cells is a cost-effective method for the production of commercial quantities of a drug substance. This kit was developed using the?final product and in-process samples for four different products from four vaccine manufacturers. These products included whole virus vaccines as well as recombinant proteins. The broadly reactive, affinity-purified, polyclonal antibodies demonstrate a high degree of conserved HCP across all Vero cell lines tested. The robustness of this sensitive and specific ELISA method facilitates successful validation for almost any product expressed in Vero cells. This kit has an LOD of ~0.7 ng/ml.

描述
重要變更通知備忘錄：Vero Cell HCP ELISA 試劑盒

新的 Vero Cell 2G HCP ELISA 試劑盒，貨號 F975 現已上市

在 Vero 細胞中表達病毒疫苗和其他治療性蛋白質是生產商業數量的藥物物質的一種經濟有效的方法。 該試劑盒是使用來自四家疫苗制造商的四種不同產品的最終產品和過程中樣品開發的。 這些產品包括全病毒疫苗和重組蛋白。 廣泛反應、親和純化的多克隆抗體在所有測試的 Vero 細胞系中都表現出高度保守的 HCP。 這種靈敏且特異的 ELISA 方法的穩健性有助于成功驗證幾乎所有在 Vero 細胞中表達的產品。 該試劑盒的 LOD 約為 0.7 ng/ml。

Kit Components
Anti-Vero Cell:HRP Conjugate
Vero Cell HCP Standards Set, A-F
Stop Solution
TBS Wash Concentrate, 20X
TMB Substrate for ELISA
Anti-Vero Cell Coated Microtiter Strips

Cygnus F410 大腸桿菌蛋白殘留檢測試劑盒 E. coli HCP ELISA Kit (F410)

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Cygnus F410 大腸桿菌蛋白殘留檢測試劑盒 E. coli HCP ELISA Kit (F410)

品牌商名稱：cygnus

Cygnus Technologies, Inc.美國賽納斯科技公司簡介

Cygnus Technologies, Inc.(美國賽納斯科技公司)是一家專為疫苗生產企業、基因工程藥企、研發部門及QA部門(品質保證部門)提供宿主細胞蛋白殘留檢測和生物工藝污染物檢測技術和產品的世界*的美國公司。包括美國和中國在內的全球生物制藥企業,生物制品生產過程中存在許多影響產品質量的殘留物,諸如特殊重組表達系統中宿主細胞蛋白(Host Cell Protein,HCP)的殘留以及生物制品加工過程中培養物殘留的污染物(Bioprocess Contaminant, BC)。美國Cygnus公司提供了種類廣泛而靈敏度極高的一系列用于檢測生物工程殘留物的HCP ELISA試劑盒和BC ELISA試劑盒等產品,在尋求*純化效果和日常質量控制過程中使用美國Cygnus公司的產品可以很方便地得到理想的實驗結果。

美國Cygnus公司提供的ELISA試劑盒產品主要包括以下兩類:

Host Cell Protein Analysis ELISA Kits(宿主細胞蛋白分析ELISA試劑盒)

其來源種屬有:哺乳動物細胞、細菌、酵母、昆蟲細胞、轉基因細胞。

Product Information
Product Type: ELISA Kit
Storage: All kit reagents 2°C to 8°C
Target Expression System: E. coli
Species Group: Bacterial
Species: E. coli
Assay format: 96 Well Plate
Time to result: ~2 hrs. 20 min
LOD: 0.2 ng/mL
LLOQ: ~1 ng/mL
Recommended Diluent: I028

英文名稱: E.coli HCP ELISA Kit
產品應用: 大腸桿菌HCP檢測
中文名稱: 大腸桿菌HCP檢測試劑盒
品牌: Cygnus
貨號: F410
規格型號: 96T/盒
保存溫度: 2~8℃
產品種類: ELISA試劑盒

Description

This kit was developed using broadly reactive polyclonal antibodies to the hundreds of different host cell proteins (HCPs) and is intended for use in determining the presence of?E. coli?HCP impurities in products manufactured by recombinant expression. Our assay validation has determined the LOD for this kit is ~0.2 ng/ml. Please use?Sample Diluent, Item No. I028, with these kits; available separately.

描述
該試劑盒是使用針對數百種不同宿主細胞蛋白 (HCP) 的廣泛反應性多克隆抗體開發的，旨在用于確定通過重組表達生產的產品中是否存在大腸桿菌 HCP 雜質。 我們的檢測驗證已確定該試劑盒的 LOD 為 ~0.2 ng/ml。 請在這些套件中使用樣品稀釋劑，貨號 I028； 可單獨購買。

Kit Components
Anti-E. coli:HRP Conjugate
E. coli HCP Standards Set, A-F
TBS Wash Concentrate, 20X
Stop Solution
TMB Substrate for ELISA
Anti-E. coli Coated Microtiter Strips

產品英文名稱:Cygnus F410 E.coli HCP ELISA Kit

產品中文名稱:Cygnus F410 大腸桿菌宿主細胞ELISA檢測試劑盒

Cygnus F410 E.coli HCP 大腸桿菌宿主細胞ELISA檢測試劑盒 產品介紹:Cygnus F410 E.coli HCP 大腸桿菌宿主細胞ELISA檢測試劑盒主要設計用于檢測產物重組表達過程中大腸桿菌宿主細胞污染,試劑盒使用的多克隆抗體對數百種HCPs具有反應性。Cygnus F410 E.coli HCP 大腸桿菌宿主細胞ELISA檢測試劑盒檢測*濃度可達到 0.2ng/mL。ELISA檢測相比Western Blot:Western Blots檢測過程復雜并需借助程序分析結果。而且,Western Blot本質上是定性分析,不能得到定量結果,檢測靈敏性也受到樣本體積的限制。Western Blot在下游純化過程中檢測產物HCPs缺乏靈敏度和特異性。而微量滴定板ELISA檢測可以克服上述限制。ELISA具備高度靈敏性,可獲得半定量結果,是純化工藝,過程控制,常規質量檢測的*選擇。

產品使用時一定要做好保護措施:

0.5M硫磺酸有強腐蝕性,要避免接觸眼睛、皮膚和衣物。檢測前所有試劑都要平衡到室溫。

  • Cygnus F410 E.coli HCP ELISA Kit 檢測原理:

大腸桿菌E. coli檢測是一個雙位點酶聯免疫檢測。樣本中包含的E. coli HCPs蛋白與標記有抗-E. coli 抗體的辣根過氧化物酶同時反應,反應發生在之前涂有一層吸附性抗-E. coli 蛋白抗體的微量滴定板中進行。免疫反應構成為夾層式結構:固相抗休-HCP-酶標記抗體。反應結束后清洗微量滴定板去除未結合反應物。添加TMB作用底物反應。微量滴定板讀數器上測定水解物濃度,水解物濃度與E. coli ?HCPs蛋白濃度成比例。用提供的標準液繪制標準曲線,根據讀數可以計算出E. coli HCPs蛋白含量。

  • Cygnus F410 大腸桿菌宿主細胞ELISA檢測試劑盒檢測過程:

取出所有試劑平衡到室溫→分別移取25μl Cygnus F410 大腸桿菌宿主細胞ELISA檢測試劑盒標準液,對照物和樣品至待測孔→移取100μl anti-E. coli:HRP(#F411)至每孔→密封滴定板,搖床室溫溫育90分鐘,180rpm→清空小孔,用紙吸干殘留液,350μl清洗緩沖液清洗4次→加100μl TMB 作用物(#F005)→室溫靜置30分鐘,不需搖床→加100μl反應終止液(#F006)→分光光度計450/650nm讀數

  • Cygnus F410 E.coli HCP ELISA Kit 檢測結果分析:

Cygnus F410 E.coli HCP ELISA Kit 標準液(0, 1, 3, 12, 40, 100ng/mL)可用于制作標準曲線,標準液濃度相當于免疫反應HCP總濃度。根據測定的小孔中標準液吸光值繪制標準曲線。Cygnus F410 E.coli HCP ELISA Kit 檢測結果數據分析可以使用計算機選配曲線,例如點到點,三次樣本函數,或四參數邏輯曲線。不要使用線性回歸分析修改樣品吸光值,這樣會出現顯著誤差。也可以手工繪圖,Y軸標注吸光值,X軸標注濃度。然后根據樣品吸光值計算出樣品中HCP濃度。(操作時每一濃度標準液都要滴定到兩個小孔中,以便計算平均分光光度值,樣品也需計算平均吸光值)。

Cygnus F410 E.coli HCP ELISA Kit 組成成份:

  • Anti- E. coli:HRP(辣根過氧化物酶標記) ? ? ? ? ? ?1x12mL ? ?F411(Product#)
  • Anti- E. coli coated微量滴定板 ? ? ? ? ? ? ? ? ? ? ? ?12×8孔 ? F412
  • E. coli HCP標準液(0, 1, 3, 12, 40, 100ng/mL) ? ? 1mL/瓶 ?F413
  • Stop Solution終止液(0.5M硫磺酸) ? ? ? ? ? ? 1x12mL F006
  • TMB (3,3’,5,5’-四甲基聯苯氨) ? ? ? ? ? ? ? ? ? ? ? ? ?1x12mL ?F005
  • Wash Concentrate清洗物(20倍濃縮) ? ? ? ? ? ? ? ?1x50mL ? ?F004

Cygnus相關試劑盒
A549 HCP, Ab, goat IgG fraction A549-PA ￥2,590.00 cygnus
A549 HCP, Ab,goat? affinity purified A549-AF ￥9,800.00 cygnus
?A549 HCP ELISA Kit ?F230 ￥8,750.00 cygnus
A549 HCP Western Blot Kit F235 ￥6,300.00 cygnus
Alkaline Phosphatase Diluent/Stabilizer ?I-045 ￥1,750.00 cygnus
?I-040 ￥2,800.00 cygnus
?Alk. Phos. Diluent/Stabilizer with HAMA?blocker ?I-045H ￥2,800.00 cygnus
?I-040H ￥4,200.00 cygnus
Anti-human IgG4:HRP IM154 ￥2,800.00 cygnus
?Bovine Plasma Proteins, Ab,?? rabbit affinity purified ?BPP04-AF ￥9,800.00 cygnus
?Bovine Serum Albumin ELISA （BSA殘留檢測試劑盒） ?F030 ￥6,930.00 cygnus
?Bovine Transferrin Antigen Concentrate ?F121G ￥2,100.00 cygnus
?Bovine Transferrin ELISA （牛轉鐵蛋白ELISA kit（宿主殘留蛋白檢測試劑盒） ?F120 ￥6,930.00 cygnus
CHO CM HCP Antigen Concentrate ?F063CM ￥4,200.00 cygnus
?CHO-CM HCP ELISA Kit （CHO宿主蛋白殘留檢測試劑盒） ?CM015 ￥8,750.00 cygnus
CHO-CM HCP Western Blot kit CM060 ￥6,300.00 cygnus
?CHO HCP ELISA Kit （CHO宿主蛋白殘留檢測試劑盒） ?F015 ￥8,750.00 cygnus
CHO HCP Western Blot kit (Lysate) F060 ￥6,300.00 cygnus
CHO Lysate HCP Antigen Concentrate F018H-10 ￥3,500.00 cygnus
E.coli HCPs, Ab, goat neat serum F117 ￥2,030.00 cygnus
E.coli HCPs, Ab, goat IgG fraction F117-PA ￥2,590.00 cygnus
E.coli HCPs, Ab, goat affinity purified AP117 ￥9,800.00 cygnus
?E. coli HCP Antigen Concentrate F413H ￥3,500.00 cygnus
?E-coli-HRP HCP ELISA kit F410 ￥8,750.00 cygnus
?E-coli-HRP HCP WB F415 ￥6,300.00 cygnus
?FG Blocker, 1L IM107 ￥1,400.00 cygnus
?Goat, anti-human IgA, 1mL IM140 ￥2,450.00 cygnus
?Goat, anti-human IgA, IgG, IgM, heavy & light chains IM102 ￥2,450.00 cygnus
?Goat, anti-human IgD, 1mL IM141 ￥2,450.00 cygnus
Goat, anti-human IgE, 1mL IM142 ￥2,450.00 cygnus
Goat, anti-human IgG, Fc gamma chain, 1mL IM143 ￥2,450.00 cygnus
?Goat, anti-human IgM, 1mL IM144 ￥2,450.00 cygnus
Goat IgG ELISA ?F210 ￥6,930.00 cygnus
Goat Milk Proteins ELISA kit F240 ￥8,750.00 cygnus
Goat Milk Proteins Western Blot kit ?F245 ￥6,300.00 cygnus
Pseudomonas fluorescens, Ab,?goat IgG fraction ?7-04PA ￥2,590.00 cygnus
?Pseudomonas fluorescens, Ab,?goat neat serum ?7-04 ￥2,030.00 cygnus
?Pseudomonas, guinea pig neat serum ?8-57 ￥2,030.00 cygnus
Saccharomyces cerevisiae HCPs, Ab, goat neat serum 82-SC ￥2,030.00 cygnus
Saccharomyces cerevisiae HCPs, Ab, goat IgG fraction 82SC-PA ￥2,590.00 cygnus
Saccharomyces cerevisiae HCPs, Ab, goat affinity purified 82SC-AF ￥19,600.00 cygnus
Saccharomyces? cerevisiae HCP ?Antigen Concentrate ?F138H ￥2,800.00 cygnus
Saccharomyces? cerevisiae HCP? ELISA?? ?F135 ￥8,750.00 cygnus
Saccharomyces cerevisiae HCP Western Blot kit F130 ￥6,300.00 cygnus
SF9 HCP ELISA ?F020 ￥8,750.00 cygnus
SF9 HCP Western Blot kit F125? ￥6,300.00 cygnus
SF9 Insect Cell HCPs, Ab, rabbit neat serum 12-SF9 ￥2,030.00 cygnus
SF9 Insect Cell HCPs, Ab, rabbit IgG fraction 12-SF9-PA ￥2,590.00 cygnus
Shigella, Ab, rabbit neat serum 40059 ￥2,030.00 cygnus
Shigella, Ab, rabbit IgG fraction 9-03-PA ￥2,590.00 cygnus
SP 2/0 HCPs, Ab, goat neat serum SP110 ￥2,030.00 cygnus
SP 2/0 HCPs, Ab, goat IgG fraction SP110-PA ￥2,590.00 cygnus
SP 2/0 HCPs, Ab, goat affinity purified SP110-AF ￥9,800.00 cygnus
?SP 2/0 HCP Antigen Concentrate ?F186C ￥4,200.00 cygnus
?SP 2/0 HCP ELISA Kit ?F180 ￥8,750.00 cygnus
SP 2/0 HCP Western Blot kit F185 ￥6,300.00 cygnus
Streptococcus pneumoniae, Ab, rabbit neat serum 8-31B ￥2,030.00 cygnus
Streptococcus pneumoniae, Ab, guinea pig IgG fraction 8-42A ￥2,030.00 cygnus
TMB & Stop Solution, substrate kit, 50 mL ?IM109 ￥1,400.00 cygnus
TMB Liquid Substrate for use in ELISA ?F027 ￥1,050.00 cygnus
TMB Liquid Substrate for use in ELISA ?F100027 ￥4,200.00 cygnus
TMB Liquid Substrate for use in Western?Blots ?F129 ￥1,050.00 cygnus
TMB Liquid Substrate for use in Western???? Blots ?F100029 ￥4,200.00 cygnus
Total Goat Milk Proteins, Ab, rabbit neat serum ?4042 ￥2,030.00 cygnus
Total Goat Milk Proteins, Ab, rabbit IgG fraction 4042-PA ￥2,590.00 cygnus
Total Goat Milk Proteins, Ab, rabbit affinity purified 4042-AF ￥9,800.00 cygnus
Vero cell HCP, Ab goat neat serum VC807 ￥2,030.00 cygnus
Vero cell HCP, Ab, goat affinity purified VC807.5-AF ￥5,600.00 cygnus
Vero cell HCP, Ab, goat affinity purified VC807-AF ￥9,800.00 cygnus
Vero cell Antigen Concentrate (for ELISA) F503H ￥4,200.00 cygnus
Vero cell Control Antigen (for Western Blot) F507 ￥1,050.00 cygnus
Vero cell HCP ELISA kit F500 ￥8,750.00 cygnus

Cygnus CHO HCP ELISA Kit, 3G (F550)中文說明書

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Cygnus CHO HCP ELISA Kit, 3G (F550)中文說明書

This product has been depleted and replaced with CHO HCP ELISA Kit, 3G (F550-1)

We have resupplied F550 due to its capture and detection antibody stock depletion.

該產品已用完并替換為 CHO HCP ELISA Kit, 3G (F550-1)
由于 F550 的捕獲和檢測抗體庫存耗盡，我們已重新供應。 如果您有任何問題或需要進一步幫助。

Product Information
Product Type: ELISA Kit
Storage: All kit reagents 2°C to 8°C
Target Expression System: CHO
Species Group: Mammalian
Species: Chinese hamster

產品信息
產品類型：ELISA 試劑盒
儲存：所有試劑盒試劑 2°C 至 8°C
目標表達系統：CHO
物種組：哺乳動物
種類：中國倉鼠

Description

Important Change Notification Memo: CHO HCP ELISA Kit, 3G Resupply

New CHO HCP ELISA Kit, 3G, Item Number F550-1 now available

This CHO HCP 3rd Generation ELISA Kit uses a more broadly reactive antibody than our earlier kits and incorporates a number of other improvements for the detection of CHO HCPs. The “CHO 3G” ELISA is more sensitive than previous kits, able to detect HCPs in the range of 100 parts per billion for a variety of antibodies and other therapeutic proteins expressed in CHO. It has been extensively qualified with a large number of drug substances and in-process samples. This data indicates the kit is truly a generic method that can likely be validated as not only a process development tool, but in most cases as a lot release test, eliminating the need to develop a custom or process specific assay. The antibody has been evaluated for reactivity to individual HCPs using the conventional 2D Western Blot as well as a method we have shown to be superior to 2D WB, which we call AAE, Antibody Affinity Extraction. AAE demonstrated the antibody is reactive to more than 1000 individual HCPs from conditioned media and cell lysates in both a CHO-S strain as well as a K1 strain. These 1000+ HCPs represent more than 98% of the total mass of protein as indicated by protein detection methods orthogonal to ELISA. As such, this antibody may overcome concerns about missing any significant individual HCPs. This ELISA is also more robust than the earlier kits showing less sample matrix interference and improved dilution linearity. If you have found the F015 and CM015 kits lacking in any way meeting your HCP detection needs, or if you are considering the need for a process-specific assay, we recommend you evaluate this 3rd Generation CHO HCP ELISA kit.

描述
重要變更通知備忘錄：CHO HCP ELISA 試劑盒、3G 補給

新的 CHO HCP ELISA 試劑盒，3G，貨號 F550-1 現已上市

此 CHO HCP 第三代 ELISA 試劑盒使用比我們早期試劑盒更廣泛的反應性抗體，并結合了許多其他改進來檢測 CHO HCP。 “CHO 3G” ELISA 比以前的試劑盒更敏感，能夠檢測 CHO 中表達的各種抗體和其他治療性蛋白的十億分之 100 范圍內的 HCP。它已通過大量原料藥和在制品樣品進行了廣泛的鑒定。該數據表明該試劑盒確實是一種通用方法，不僅可以作為工藝開發工具進行驗證，而且在大多數情況下可以作為批量放行測試，從而無需開發定制或工藝特定的檢測方法。已使用傳統的 2D Western Blot 以及我們已證明優于 2D WB 的方法（我們稱之為 AAE，抗體親和提?。┰u估了該抗體對單個 HCP 的反應性。 AAE 證明該抗體對來自 CHO-S 菌株和 K1 菌株的條件培養基和細胞裂解物的 1000 多個單獨的 HCP 具有反應性。與 ELISA 正交的蛋白質檢測方法表明，這 1000 多個 HCP 占蛋白??質總質量的 98% 以上。因此，這種抗體可以克服對遺漏任何重要個體 HCP 的擔憂。這種 ELISA 也比早期的試劑盒更穩健，顯示出更少的樣品基質干擾和改進的稀釋線性。如果您發現 F015 和 CM015 試劑盒在任何方面都無法滿足您的 HCP 檢測需求，或者如果您正在考慮需要特定過程的檢測，我們建議您評估這款第三代 CHO HCP ELISA 試劑盒。

Kit Components 試劑盒組成
Anti-CHO:HRP Conjugate, 3G
CHO HCP Standards Set, A-F, 3G
Stop Solution
TBS Wash Concentrate, 20X
TMB Substrate for ELISA
Anti-CHO Coated Microtiter Strips

Product FAQs 產品問與答

Why Perform Dual Wavelength Analysis?

The protocols in our ELISA kits recommend the use of dual wavelength analysis for microtiter plates readers appropriately equipped. For alkaline phosphatase – PNPP based assays the two wavelengths are 405 nm and 492 nm. For horse radish peroxidase – TMB based assays the wavelengths are 450 nm and 630 nm. In both assay types the first wavelength listed, also termed the “test wavelength”, is critical and thus your microtiter plate reader should be equipped with the appropriate 405 nm and 450 nm filters. It is not absolutely necessary to use dual wavelength analysis with any of our assays and thus the second wavelength (492 nm and 630 nm – also called the “reference wavelengths”) are optional. In theory, the use of dual wavelength analysis should provide better precision. Microtiter plate readers with the appropriate software will automatically subtract the reference wavelength absorbance from the test wavelength absorbance. In theory dual wavelength analysis can help to overcome any non-wavelength specific imperfections in the plate. In practice, because the optical quality of our microtiter plates is excellent, there is usually no significant statistical improvement in the data by subtracting out the reference absorbance from the test absorbance. Most plate readers are equipped to perform dual wavelength analysis. Since the reference wavelength is not critical, microtiter plate readers with reference filters close to those we recommend can be substituted. For example, you may use a 490 nm or a 500 nm filter in place of the 492 nm or a 600 nm or 650 nm filter in place of the 630 nm filter.

為什么要進行雙波長分析？
我們的 ELISA 試劑盒中的方案建議對適當配備的微量滴定板讀數器使用雙波長分析。對于堿性磷酸酶 – 基于 PNPP 的分析，兩個波長是 405 nm 和 492 nm。對于辣根過氧化物酶 – 基于 TMB 的檢測，波長為 450 nm 和 630 nm。在這兩種檢測類型中，列出的第一個波長（也稱為“測試波長”）至關重要，因此您的微量滴定板讀數器應配備適當的 405 nm 和 450 nm 濾光片。在我們的任何檢測中使用雙波長分析并不是絕對必要的，因此第二個波長（492 nm 和 630 nm – 也稱為“參考波長”）是可選的。理論上，使用雙波長分析應該提供更好的精度。帶有適當軟件的微量滴定板讀數器會自動從測試波長吸光度中減去參考波長吸光度。理論上，雙波長分析可以幫助克服印版中任何非波長特定的缺陷。在實踐中，由于我們的微量滴定板的光學質量非常好，從測試吸光度中減去參考吸光度通常不會對數據進行顯著的統計改進。大多數讀板機都配備執行雙波長分析。由于參考波長并不重要，因此可以替換帶有接近我們推薦的參考濾光片的微量滴定板讀數器。例如，您可以使用 490 nm 或 500 nm 濾光片代替 492 nm 或 600 nm 或 650 nm 濾光片代替 630 nm 濾光片。

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Cygnus CHO HCP ELISA Kit (F015)試劑盒說明書

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Cygnus CHO HCP ELISA Kit (F015)試劑盒說明書

Cygnus試劑盒Cygnus technologies熱銷產品
Cygnus熱銷產品：cygnus f975、cygnus f410檢測試劑盒、cygnus f550
品牌商名稱：cygnus
公司官網：

Product Information

Product Type:ELISA Kit
Storage:All kit reagents 2°C to 8°C
Target Expression System:CHO
Species Group:Mammalian
Species:Chinese hamster
Assay format:96 Well Plate
Time to result:~3 hrs. 50 min. or ~5 hrs. 50 min
LOD:400 pg/mL
LLOQ:~4 ng/mL , or ~1 ng/mL
Recommended Diluent:I028

Description

The antibodies in this kit were generated against and affinity-purified from a cell lysate of CHO cells grown in serum supplemented media.

All three Cygnus CHO HCP kits recognize a vast majority of the same HCPs, but because the potential exists that the quantitative value reported for a given sample type may differ between the kits, we cannot predict which kit will be most suitable for your applications. Formal qualification must be performed on each different product to determine which of the three kits is best. For labs looking at CHO HCP impurities for the first time, use our CHO HCP ELISA, 3G (F550). If this method proves inadequate, then suggest evaluate CHO HCP kits CM015 and F015. Please use?Sample Diluent, Item No. I028, with these kits; available separately.

描述
該試劑盒中的抗體是針對在補充有血清的培養基中生長的 CHO 細胞的細胞裂解物產生并親和純化的。

所有三個 Cygnus CHO HCP 試劑盒都可以識別絕大多數相同的 HCP，但由于可能存在針對給定樣品類型報告的定量值可能因試劑盒而異，我們無法預測哪種試劑盒最適合您的應用。 必須對每種不同的產品進行正式鑒定，以確定三個套件中的哪一個是最好的。 對于首次檢測 CHO HCP 雜質的實驗室，請使用我們的 CHO HCP ELISA, 3G (F550)。 如果這種方法被證明不合適，那么建議評估 CHO HCP 試劑盒 CM015 和 F015。 請在這些套件中使用樣品稀釋劑，貨號 I028； 可單獨購買。

Kit Components 試劑盒成分：
Anti-CHO:Alkaline Phosphatase Conjugate
CHO HCP Standards Set, A-F
TBS Wash Concentrate, 20X
PNPP Substrate
Anti-CHO Coated Microtiter Strips

Product FAQs? 產品問與答：

  • Can the ELISA Protocol in the Product Insert be Modified?

    The assay protocol?recommended in the kits’ product insert have been developed with consideration given to typical limits of detection required in bioprocess impurity analysis. The assays are very robust with respect to protocol options and thus it is possible to modify the protocol to achieve performance parameters more optimal for your analytical needs. Sample volume, incubation times, and use of various sequential (forward or reverse sequential) schemes can lead to significant changes in sensitivity, reduction of non-specific sample matrix affects, or greater upper analytical range. If you change the protocol, it will be necessary to qualify that those changes achieve acceptable accuracy, specificity, and precision. Please refer to the section below on “How to Qualify HCP & Impurity Assays.” For advice on how best to modify an assay to meet your objectives you are encouraged to contact our?Technical Services Department.

    產品插頁中的 ELISA 方案可以修改嗎？
    試劑盒產品插頁中推薦的分析方案是在考慮到生物過程雜質分析所需的典型檢測限的情況下開發的。 這些分析在方案選項方面非常穩健，因此可以修改方案以實現更適合您的分析需求的性能參數。 樣品體積、孵育時間和各種順序（正向或反向順序）方案的使用會導致靈敏度顯著變化、非特異性樣品基質影響的減少或更高的分析范圍。 如果您更改協議，則有必要確定這些更改達到可接受的準確性、特異性和精密度。 請參閱下面的“如何鑒定 HCP 和雜質檢測”部分。 如需有關如何最好地修改檢測以達到您的目標的建議，我們鼓勵您聯系我們的技術服務部門。

    Calibration of HCP assays

    The absolute quantitation of HCP assays is exceedingly difficult for several reasons. First of all, there is no recognized reference preparation of HCP against which we can calibrate our assays. While ELISA is inherently a quantitative method when applied to a single analyte, ELISAs that attempt to measure simultaneously all of the hundreds of potential HCP impurities in the same well using a single reporter/detector system are at best semi-quantitative assays. Many arbitrary choices and assumptions are used when preparing anti-HCP antibodies and later the choice of material to use when preparing “standards”.

    How to obtain the HCP material for immunogen is the first choice that must be made.

    • Do we use a null cell line or a mock transfected cell line or one with the actual product plasmid and what differences in HCP might we see from each??
    • At what point in the purification process do we obtain the HCPs for the immunogen or standards?

    In almost every case the array and relative concentrations of HCPs in final product are different from the HCP in the ELISA standards.

    • How does one assign a total HCP concentration to an indeterminant mixture of many proteins with different molecular weights?
    • What effect will the different affinities of antibodies to various HCPs as well as the different concentrations of those antibodies have on the ability of the assay to be quantitative?
    • How do we know that we have antibody to all the HCPs present in a given sample type??

    All of these questions and arbitrary choices mean HCP assays will at best be semi-quantitative methods capable of making only relative estimates of HCP concentrations from one sample to the next. We estimate the potential error in reporting an HCP level in a sample that has a different array of HCPs from the standards to be as high as 4-fold even if you have a very good broadly reactive antibody. The commonly expressed theoretical concern is that HCP assays might under-estimate results due to incomplete coverage of all HCPs. In reality, the quantitative errors in HCP assays due to the arbitrary choices and fundamental limitations of the method as discussed above can result in either over-estimation or under-estimation and tend to do so to about the same degree. In our experience, a well generated and affinity purified antibody will react to more than 70% of individual HCPs as demonstrated by traditional 2D Western blot correlated to silver stain. What is more important than the number of individual proteins with antibody reactivity, is the total mass of those proteins that are detected. In our experience and as you might expect from theoretical considerations, the proteins in highest concentration have the best chance of generating an antibody. As such, an antibody with about 70% reactivity to an individual HCP will react with those proteins that account for more than 95% of the total mass of HCP. Given this level of uncertainty in the absolute HCP concentration, the most important criteria by which we can judge any HCP assay is on other objective parameters by which any analytical method should be qualified or validated. Those criteria include specificity and accuracy as demonstrated by sample dilution linearity and spike recovery experiments, precision, robustness, and sensitivity. Provided the assay has sensitivity to detect at least a relative portion of the HCP in your final drug substance and the assay also meets the other objective analytical parameter specifications, such an assay is a valuable analytical method capable of demonstrating process control and reporting relative levels of HCP contamination from lot to lot as a release test.

    Due to the impracticality of obtaining real final product HCPs that co-purify with product down to the final step, most of our HCP assays will utilize a source of HCPs from very upstream in the purification process. Those HCPs typically do not come from the actual product cell line but rather a null cell or mock transfected cell line. Once we have decided on the source material for the kit standards we first perform some partial purifications to remove non-HCP materials such as growth media additives, buffer salts and extraction reagents. Our initial approach to calibrate this processed material is to simply perform a BCA total protein assay using bovine serum albumin (BSA) as an arbitrary standard. When standards made with the HCP material calibrated by BCA give reasonable “stoichiometric agreement” with the amounts of antibody used in the ELISA then we feel the calibration by BCA is good enough. What is meant by “stoichiometric agreement” is that we know how much antibody is used in the ELISA. It is the quantity of antibody that actually dictates the analytical range and dose response curve of the ELISA. If we assume an average molecular weight for the total HCPs, then we can reasonably estimate HCP concentrations across the valid analytical range of the assay. When the BCA assay concentrations do not reasonably approximate the ELISA stoichiometry, we process the HCP material further. Such processing involves various purification steps to remove components registering in the BCA assay but that are in fact not HCP or at least not immunoreactive HCPs. This processing might also involve affinity purification against the anti-HCP antibody. The purification stops as soon as the BCA concentration gives a realistic stoichiometric agreement in the ELISA.

    HCP 檢測的校準
    由于幾個原因，HCP 測定的絕對定量非常困難。首先，沒有公認的 HCP 參考制劑可以用來校準我們的檢測。雖然 ELISA 在應用于單個分析物時本質上是一種定量方法，但嘗試使用單個報告器/檢測器系統同時測量同一孔中的所有數百種潛在 HCP 雜質的 ELISA 充其量是半定量分析。在制備抗 HCP 抗體時使用了許多任意選擇和假設，然后在制備“標準”時選擇使用的材料。

    如何獲得免疫原的HCP材料是必須要做的第一選擇。

    我們是使用無效細胞系還是模擬轉染細胞系，還是使用帶有實際產品質粒的細胞系？我們可以從中看到 HCP 的哪些差異？
    我們在純化過程中的哪個階段獲得免疫原或標準品的 HCP？
    在幾乎所有情況下，最終產品中 HCP 的陣列和相對濃度都不同于 ELISA 標準中的 HCP。

    如何將總 HCP 濃度分配給許多具有不同分子量的蛋白質的不確定混合物？
    抗體對各種 HCP 的不同親和力以及這些抗體的不同濃度對測定的定量能力有什么影響？
    我們如何知道我們對給定樣本類型中存在的所有 HCP 都有抗體？
    所有這些問題和任意選擇意味著 HCP 測定充其量只能是半定量方法，只能對從一個樣品到下一個樣品的 HCP 濃度進行相對估計。我們估計，即使您擁有非常好的廣泛反應性抗體，在 HCP 陣列與標準不同的樣本中報告 HCP 水平的潛在錯誤也會高達 4 倍。普遍表達的理論擔憂是，由于對所有 HCP 的覆蓋不完整，HCP 檢測可能會低估結果。實際上，由于上??述方法的任意選擇和基本限制導致的 HCP 測定中的定量誤差可能導致高估或低估，并且傾向于以大致相同的程度這樣做。根據我們的經驗，與銀染色相關的傳統 2D 蛋白質印跡證明，良好生成和親和純化的抗體將與 70% 以上的單個 HCP 發生反應。比具有抗體反應性的單個蛋白質的數量更重要的是檢測到的這些蛋白質的總質量。根據我們的經驗以及您可能從理論上的考慮，最高濃度的蛋白質最有可能產生抗體。因此，對單個 HCP 具有約 70% 反應性的抗體將與那些占 HCP 總質量 95% 以上的蛋白質發生反應。鑒于 HCP 絕對濃度的這種不確定性水平，我們判斷任何 HCP 測定的最重要標準是其他客觀參數，任何分析方法都應通過這些客觀參數進行鑒定或驗證。這些標準包括由樣品稀釋線性和加標回收實驗證明的特異性和準確性、精密度、穩健性和靈敏度。如果該測定對檢測最終原料藥中至少一部分 HCP 具有敏感性，并且該測定還符合其他客觀分析參數規范，則該測定是一種有價值的分析方法，能夠證明過程控制并報告 HCP 的相對水平。批次間 HCP 污染作為放行測試。

    由于獲得與產品共同純化直至最后一步的真正最終產品 HCP 是不切實際的，我們的大多數 HCP 測定將利用純化過程中非常上游的 HCP 來源。這些 HCP 通常不是來自實際的產品細胞系，而是來自空細胞或模擬轉染細胞系。一旦我們確定了試劑盒標準的來源材料，我們首先會進行一些部分純化以去除非 HCP 材料，例如生長培養基添加劑、緩沖鹽和提取試劑。我們校準這種加工材料的初始方法是使用牛血清白蛋白 (BSA) 作為任意標準簡單地執行 BCA 總蛋白測定。當使用 BCA 校準的 HCP 材料制成的標準與 ELISA 中使用的抗體量給出合理的“化學計量一致性”時，我們認為 BCA 的校準已經足夠好。 “化學計量一致性”的意思是我們知道在 ELISA 中使用了多少抗體?？贵w的數量實際上決定了 ELISA 的分析范圍和劑量反應曲線。如果我們假設總 HCP 的平均分子量，那么我們可以合理地估計有效分析范圍內的 HCP 濃度的化驗。 當 BCA 測定濃度不能合理地接近 ELISA 化學計量時，我們會進一步處理 HCP 材料。 這種處理涉及各種純化步驟，以去除在 BCA 測定中登記但實際上不是 HCP 或至少不是免疫反應性 HCP 的成分。 該處理還可能涉及針對抗 HCP 抗體的親和純化。 一旦 BCA 濃度在 ELISA 中給出真實的化學計量一致，純化就會停止。

    What is the Storage and Stability of ELISA Kits?

    Cygnus kits and reagents have been formulated to withstand shipment and/or temporary storage under ambient temperature conditions without any significant deterioration. Orders within North America are shipped next day delivery service without cold packs. International orders are shipped with cold packs to protect from extreme heat.? Although stability studies have demonstrated that Cygnus kits can be held several weeks at ambient temperatures without any significant deterioration, we recommend storage at 2-8°C upon receipt of the products.? ?Our components have been challenged at elevated temperature excursions, addressing concerns over extreme summertime temperatures, delays in shipping, custom clearances and/or refrigeration problems at the customer locations.

    Should you have concerns regarding the performance of kits that have been subject to temperature deviations, we recommend an analysis of your control samples and historical specifications that determine the integrity of the kit. If the curve absorbances, sensitivity, and controls are within range, then the kit is acceptable for use.

    ELISA 試劑盒的儲存和穩定性如何？
    Cygnus 試劑盒和試劑經過精心配制，可在環境溫度條件下經受運輸和/或臨時儲存，而不會出現任何顯著變質。北美地區的訂單在沒有冷包的情況下第二天發貨。國際訂單隨附冷袋以防止過熱。雖然穩定性研究表明 Cygnus 套件可以在環境溫度下保存數周而不會出現任何明顯的劣化，但我們建議在收到產品后將其儲存在 2-8°C。我們的組件在高溫偏移中面臨挑戰，解決了對夏季極端溫度、運輸延遲、海關清關和/或客戶所在地制冷問題的擔憂。

    如果您對受溫度偏差影響的試劑盒的性能有疑慮，我們建議您分析您的對照樣品和確定試劑盒完整性的歷史規格。如果曲線吸光度、靈敏度和對照在范圍內，則可以使用該試劑盒。

    Which Cygnus CHO HCP kit to use: F015, CM015, F550 or F550-1?

    Cygnus currently offers 3 ELISA kits to measure CHO HCPs. Kit F015, the first kit developed more than 18 years ago, utilized a mild lysate of washed CHO cells for the immunogen and for affinity purification of the antibody, as well as for kit standards. This mild lysis procedure generated an array of HCPs similar to those found in culture media after cells have been grown to densities and viabilities similar to product harvest. Cygnus continues to support this kit as many companies have qualified it for their process. However, for first time users we suggest you first try our F550-1 3rd Generation CHO ELISA kit as it has been qualified for many products and has proven to be a broadly reactive generic kit able to detect downstream HCPs in most processes.

    Kit CM015 was developed 16 years ago with the rationale that since most CHO expressed products are released into the growth media (conditioned media) an antibody and/or assay made to those HCPs found in conditioned media should be more specific than a cell lysate assay. However, the CM015 kit has been demonstrated to be more process specific and thus is not a generic kit that might be applied across most products and processes.

    The F550 3rd Generation kit used an even more reactive antibody than earlier kits and incorporates significant improvements in the assay methodology. The antibody used in that kit has been evaluated for coverage by orthogonal method such as Antibody Affinity Extraction (AAE) with 2D-PAGE/Silver Stain. The F550 kit, when supported by comprehensive qualification of the ELISA itself along with data from orthogonal methods like AAE and Mass Spectrometry, has been successfully used as a lot release test in several processes. Due to the depletion of anti-CHO HCP Antibody used in F550 kit, this kit has been replaced with the resupply F550-1 CHO HCP ELISA, 3G.

    Starting from January 1, 2021, the 3rd generation CHO HCP ELISA Kit, Item Number F550-1, replaced CHO HCP ELISA Kit, 3G, Item Number F550. Cygnus manufactured, characterized, and qualified large pools of the new reagents that replaced reagents used in F550 kit. The new antibodies were developed and purified in the same way as the original critical reagents in the F550 Kit. The coverage of the new antibodies to the CHO HCP antigen was determined to be ~86% by Antibody Affinity Extraction with 2D-PAGE Silver Stain and 97% by AAE with MS. The antibody in F550-1 kit are very similar to the antibody in the depleted F550 kit. More importantly, Cygnus Technologies has demonstrated the equivalency of the new antibodies (3G-0016-1-AF) supporting 3G CHO ELISA Kit (F550-1) using a panel of CHO samples from a number of independent biomanufacturing processes.?Download Cygnus Case Study?comparing reactivity of anti-CHO HCP Antibody from F550 (3G-0016-AF) and F550-1 (3G-0016-1-AF) to CHO HCPs.

    Contact our expert technical staff to determine if the F550-1 kit is satisfactory for your product and process or if a process-specific method is needed.

    使用哪個 Cygnus CHO HCP 試劑盒：F015、CM015、F550 或 F550-1？
    Cygnus 目前提供 3 種 ELISA 試劑盒來測量 CHO HCP。試劑盒 F015 是 18 多年前開發的第一個試劑盒，它利用洗滌過的 CHO 細胞的溫和裂解物作為免疫原和抗體的親和純化以及試劑盒標準品。這種溫和的裂解程序在細胞生長到與產品收獲相似的密度和活力后，產生了一系列與培養基中發現的 HCP 相似的 HCP。 Cygnus 繼續支持該套件，因為許多公司已經對其流程進行了認證。但是，對于初次使用的用戶，我們建議您首先嘗試我們的 F550-1 第三代 CHO ELISA 試劑盒，因為它已通過許多產品的認證，并且已被證明是一種廣泛反應的通用試劑盒，能夠在大多數過程中檢測下游 HCP。

    試劑盒 CM015 是 16 年前開發的，其基本原理是，由于大多數 CHO 表達產物被釋放到生長培養基（條件培養基）中，因此對條件培養基中發現的 HCP 進行的抗體和/或測定應該比細胞裂解物測定更具特異性。然而，CM015 套件已被證明更具工藝特異性，因此不是可應用于大多數產品和工藝的通用套件。

    F550 第三代試劑盒使用比早期試劑盒更具反應性的抗體，并在檢測方法上進行了重大改進。該試劑盒中使用的抗體已通過正交方法評估覆蓋率，例如使用 2D-PAGE/Silver Stain 進行抗體親和提取 (AAE)。 F550 試劑盒在 ELISA 本身的全面鑒定以及來自 AAE 和質譜等正交方法的數據的支持下，已成功用作多個過程中的批放行測試。由于 F550 試劑盒中使用的抗 CHO HCP 抗體耗盡，該試劑盒已替換為補給 F550-1 CHO HCP ELISA, 3G。

    從 2021 年 1 月 1 日起，第三代 CHO HCP ELISA 試劑盒，貨號 F550-1，取代了 CHO HCP ELISA 試劑盒，3G，貨號 F550。 Cygnus 制造、表征和鑒定了大量新試劑，以取代 F550 試劑盒中使用的試劑。新抗體的開發和純化方法與 F550 試劑盒中的原始關鍵試劑相同。使用 2D-PAGE Silver Stain 進行抗體親和提取確定新抗體對 CHO HCP 抗原的覆蓋率約為 86%，而 AAE 和 MS 的覆蓋率確定為 97%。 F550-1 試劑盒中的抗體與耗盡的 F550 試劑盒中的抗體非常相似。更重要的是，Cygnus Technologies 使用來自多個獨立生物制造過程的一組 CHO 樣本證明了支持 3G CHO ELISA 試劑盒 (F550-1) 的新抗體 (3G-0016-1-AF) 的等效性。下載 Cygnus 案例研究，比較來自 F550 (3G-0016-AF) 和 F550-1 (3G-0016-1-AF) 的抗 CHO HCP 抗體與 CHO HCP 的反應性。

    請聯系我們的專業技術人員以確定 F550-1 套件是否適合您的產品和工藝，或者是否需要特定工藝的方法。

Cygnus試劑盒Cygnus technologies熱銷產品

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Cygnus試劑盒Cygnus technologies熱銷產品

Cygnus熱銷產品：cygnus f975、cygnus f410檢測試劑盒、cygnus f550

品牌商名稱：cygnus
聯系地址：4701 Southport Supply Rd., SE, Suite 7 Southport, NC 28461
公司官網：http://cygnustechnologies.com

Cygnus生物公司是一家專為疫苗生產企業、基因工程藥企、研發部門及QA部門提供污染物檢測產品的世界**的美國公司。

Cygnus公司產品主要包括：

檢測宿主細胞蛋白殘留和生物制品生產過程污染物(Elisa?試劑盒）；? 檢測并鑒定常用重組表達系統中的宿主細胞蛋白（WesternBlotting?試劑盒）為生物醫藥行業提供檢測生物制品免疫原性的方法；檢測微生物的抗體。

We offer a comprehensive menu of well-validated, convenient to use microtiter plate ELISA methods to detect Host Cell Proteins from specific recombinant expression systems and bioprocess contaminants used in culture media as well as transgenic expression systems in goats and cattle. We develop and routinely manufacture, by cGMP methods, ?ELISA and Western Blot kits to contaminants specific to more than 50 different expression systems. Our extensive list of ancillary reagents is useful in developing and validating your product and process-specific assays.
Cygnus試劑盒Cygnus technologies熱銷產品

貨號???????? 中文名稱
F230???????????????????? A549 宿主蛋白檢測試劑盒
F510???????????????????? BHK 宿主蛋白檢測試劑盒
F015???????????????????? CHO宿主蛋白檢測試劑盒
CM015???????????????? 第二代CHO宿主蛋白檢測試劑盒
F550???????????????????? 第三代CHO宿主蛋白檢測試劑盒
F650???????????????????? HEK 293 宿主蛋白檢測試劑盒
F300???????????????????? MRC5 宿主蛋白檢測試劑盒
F220???????????????????? NS/0 宿主蛋白檢測試劑盒
F530???????????????????? PER.C6 細胞宿主蛋白檢測試劑盒
F180???????????????????? SP 2/0宿主蛋白檢測試劑盒
F500???????????????????? Vero 細胞 宿主蛋白檢測試劑盒
F450???????????????????? 熒光假單胞菌宿主蛋白檢測試劑盒
F320???????????????????? 金黃色葡萄球菌宿主蛋白檢測試劑盒
F410???????????????????? E. coli 宿主蛋白檢測試劑盒
F490???????????????????? 乳酸乳球菌宿主蛋白檢測試劑盒
F140???????????????????? 畢赤酵母宿主蛋白檢測試劑盒
F135???????????????????? 釀酒酵母宿主蛋白檢測試劑盒
F020???????????????????? SF9細胞宿主蛋白檢測試劑盒
F240???????????????????? 總羊奶蛋白殘留檢測試劑盒
F070???????????????????? 牛IgG蛋白檢測試劑盒
F290???????????????????? 牛血清蛋白檢測試劑盒
F030???????????????????? 牛血清白蛋白檢測試劑盒
F120???????????????????? 牛轉鐵蛋白檢測試劑盒
F210???????????????????? 山羊IgG檢測試劑盒
F055???????????????????? 人白蛋白檢測試劑盒
F165???????????????????? 人IgA檢測試劑盒
F160???????????????????? 人總IgG檢測試劑盒
F170???????????????????? 人IgM檢測試劑盒
F035N????????????????? 人轉鐵蛋白檢測試劑盒
F040???????????????????? 胰島素檢測試劑盒
F280???????????????????? 超敏胰島素檢測試劑盒
F049???????????????????? 小鼠總IgG檢測試劑盒
F045???????????????????? 小鼠IgG1檢測試劑盒
F046???????????????????? 小鼠IgG2a檢測試劑盒
F047???????????????????? 小鼠IgG2b檢測試劑盒
F090???????????????????? 小鼠IgM檢測試劑盒
F050???????????????????? Protein A殘留檢測試劑盒
F050H????????????????? Protein A殘留檢測試劑盒
F400???????????????????? Protein A殘留檢測試劑盒

Cygnus F975 宿主細胞蛋白檢測試劑盒 Vero Cell 2G HCP ELISA KIT

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Cygnus F975 宿主細胞蛋白檢測試劑盒 Vero Cell 2G HCP ELISA KIT

Description

Expression of viral vectors and vaccines in Vero cells is a cost-effective method for production of commercial quantities of a drug substance. The manufacturing and purification process of these products leaves the potential for impurities by HCPs from Vero cells. Such impurities can reduce the efficacy of the therapeutic or vaccine agent and result in adverse toxic or immunological reactions. Thus, it is desirable to reduce HCP impurities to the lowest levels practical.
This kit was developed using the antibodies that have been generated against and affinity purified using mild lysate of Vero cells. This simple to use, robust, and semi-quantitative ELISA is a sensitive and specific method to aid in optimal purification process development, process control, routine quality control, and product release testing. This kit is “generic” in the sense that it is intended to react with a majority of the HCPs that could pollute the product independent of the purification process.
This Vero Cell HCP ELISA has an LOD of ~1.4 ng/ml.

描述
在 Vero 細胞中表達病毒載體和疫苗是生產商業量藥物的一種經濟有效的方法。這些產品的制造和純化過程留下了來自 Vero 細胞的 HCP 產生雜質的可能性。此類雜質會降低治療劑或疫苗劑的功效并導致不利的毒性或免疫反應。因此，希望將 HCP 雜質減少到實際的最低水平。
該試劑盒是使用針對 Vero 細胞的溫和裂解物產生并親和純化的抗體開發的。這種簡單易用、穩健且半定量的 ELISA 是一種靈敏且特異的方法，有助于優化純化工藝開發、工藝控制、常規質量控制和產品放行測試。該試劑盒是“通用的”，因為它旨在與大多數可能污染產品的 HCP 反應，而與純化過程無關。
此 Vero Cell HCP ELISA 的 LOD 約為 1.4 ng/ml。

Cygnus F183A-100 SP2/0樣品稀釋液

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Cygnus F183A-100 SP2/0樣品稀釋液
Cygnus試劑盒Cygnus technologies熱銷產品
Cygnus熱銷產品：cygnus f975、cygnus f410檢測試劑盒、cygnus f550
上海金畔生物代理Cygnus品牌試劑盒等產品，歡迎訪問Cygnus官網或者咨詢我們獲取相關產品試劑盒說明書等其他產品信息。
品牌：Cygnus
貨號：F183A-100
產品名稱：SP2/0樣品稀釋液
規格：100 mL

Cygnus I028-100 樣品稀釋液

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Cygnus I028-100 樣品稀釋液
Cygnus試劑盒Cygnus technologies熱銷產品
Cygnus熱銷產品：cygnus f975、cygnus f410檢測試劑盒、cygnus f550
上海金畔生物代理Cygnus品牌試劑盒等產品，歡迎訪問Cygnus官網或者咨詢我們獲取相關產品試劑盒說明書等其他產品信息。
品牌：Cygnus
貨號：I028-100
產品名稱：樣品稀釋液
規格：100 mL

Cygnus F510 BHK宿主蛋白殘留檢測試劑盒

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Cygnus F510 BHK宿主蛋白殘留檢測試劑盒
Cygnus試劑盒Cygnus technologies熱銷產品
Cygnus熱銷產品：cygnus f975、cygnus f410檢測試劑盒、cygnus f550
上海金畔生物代理Cygnus品牌試劑盒等產品，歡迎訪問Cygnus官網或者咨詢我們獲取相關產品試劑盒說明書等其他產品信息。
品牌：Cygnus
貨號：F510
產品名稱：BHK宿主蛋白殘留檢測試劑盒
規格：1 kit

Cygnus CM060 CHO宿主蛋白WB檢測試劑盒

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Cygnus CM060 CHO宿主蛋白WB檢測試劑盒
Cygnus試劑盒Cygnus technologies熱銷產品
Cygnus熱銷產品：cygnus f975、cygnus f410檢測試劑盒、cygnus f550
上海金畔生物代理Cygnus品牌試劑盒等產品，歡迎訪問Cygnus官網或者咨詢我們獲取相關產品試劑盒說明書等其他產品信息。
品牌：Cygnus
貨號：CM060
產品名稱：CHO宿主蛋白WB檢測試劑盒
規格：1 kit

Cygnus F223A-100 NS/0樣品稀釋液

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Cygnus F223A-100 NS/0樣品稀釋液
Cygnus試劑盒Cygnus technologies熱銷產品
Cygnus熱銷產品：cygnus f975、cygnus f410檢測試劑盒、cygnus f550
上海金畔生物代理Cygnus品牌試劑盒等產品，歡迎訪問Cygnus官網或者咨詢我們獲取相關產品試劑盒說明書等其他產品信息。
品牌：Cygnus
貨號：F223A-100
產品名稱：NS/0樣品稀釋液
規格：100 mL

Cygnus I028-500 樣品稀釋液

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Cygnus I028-500 樣品稀釋液
Cygnus試劑盒Cygnus technologies熱銷產品
Cygnus熱銷產品：cygnus f975、cygnus f410檢測試劑盒、cygnus f550
上海金畔生物代理Cygnus品牌試劑盒等產品，歡迎訪問Cygnus官網或者咨詢我們獲取相關產品試劑盒說明書等其他產品信息。
品牌：Cygnus
貨號：I028-500
產品名稱：樣品稀釋液
規格：500 mL

Cygnus F530 PER.C6?宿主蛋白殘留檢測試劑盒

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Cygnus F530 PER.C6?宿主蛋白殘留檢測試劑盒
Cygnus試劑盒Cygnus technologies熱銷產品
Cygnus熱銷產品：cygnus f975、cygnus f410檢測試劑盒、cygnus f550
上海金畔生物代理Cygnus品牌試劑盒等產品，歡迎訪問Cygnus官網或者咨詢我們獲取相關產品試劑盒說明書等其他產品信息。
品牌：Cygnus
貨號：F530
產品名稱：PER.C6?宿主蛋白殘留檢測試劑盒
規格：1 kit

Cygnus F145 畢赤酵母宿主蛋白WB檢測試劑盒

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Cygnus F145 畢赤酵母宿主蛋白WB檢測試劑盒
Cygnus試劑盒Cygnus technologies熱銷產品
Cygnus熱銷產品：cygnus f975、cygnus f410檢測試劑盒、cygnus f550
上海金畔生物代理Cygnus品牌試劑盒等產品，歡迎訪問Cygnus官網或者咨詢我們獲取相關產品試劑盒說明書等其他產品信息。
品牌：Cygnus
貨號：F145
產品名稱：畢赤酵母宿主蛋白WB檢測試劑盒
規格：1 kit

Cygnus F233A-100 A549樣品稀釋液

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Cygnus F233A-100 A549樣品稀釋液
Cygnus試劑盒Cygnus technologies熱銷產品
Cygnus熱銷產品：cygnus f975、cygnus f410檢測試劑盒、cygnus f550
上海金畔生物代理Cygnus品牌試劑盒等產品，歡迎訪問Cygnus官網或者咨詢我們獲取相關產品試劑盒說明書等其他產品信息。
品牌：Cygnus
貨號：F233A-100
產品名稱：A549樣品稀釋液
規格：100 mL

Cygnus I094-500 樣品稀釋液

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Cygnus I094-500 樣品稀釋液
Cygnus試劑盒Cygnus technologies熱銷產品
Cygnus熱銷產品：cygnus f975、cygnus f410檢測試劑盒、cygnus f550
上海金畔生物代理Cygnus品牌試劑盒等產品，歡迎訪問Cygnus官網或者咨詢我們獲取相關產品試劑盒說明書等其他產品信息。
品牌：Cygnus
貨號：I094-500
產品名稱：樣品稀釋液
規格：500 ml

Cygnus F800 MDCK宿主蛋白殘留檢測試劑盒

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Cygnus F800 MDCK宿主蛋白殘留檢測試劑盒
Cygnus試劑盒Cygnus technologies熱銷產品
Cygnus熱銷產品：cygnus f975、cygnus f410檢測試劑盒、cygnus f550
上海金畔生物代理Cygnus品牌試劑盒等產品，歡迎訪問Cygnus官網或者咨詢我們獲取相關產品試劑盒說明書等其他產品信息。
品牌：Cygnus
貨號：F800
產品名稱：MDCK宿主蛋白殘留檢測試劑盒
規格：1 kit

Cygnus D108-30 DNA提取緩沖液

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Cygnus D108-30 DNA提取緩沖液
Cygnus試劑盒Cygnus technologies熱銷產品
Cygnus熱銷產品：cygnus f975、cygnus f410檢測試劑盒、cygnus f550
上海金畔生物代理Cygnus品牌試劑盒等產品，歡迎訪問Cygnus官網或者咨詢我們獲取相關產品試劑盒說明書等其他產品信息。
品牌：Cygnus
貨號：D108-30
產品名稱：DNA提取緩沖液
規格：30 ml