Harlan TD.02028 Atherogenic Rodent Diet 致動脈粥樣硬化的嚙齒動物飲食

發表于2022年4月7日由上海金畔生物科技有限公司

Harlan動物飼料

上海金畔生物供應Harlan TD.02028 Atherogenic Rodent Diet 致動脈粥樣硬化的嚙齒動物飲食，歡迎咨詢我們或者訪問Harlan官網查詢相關飼料報價說明書等信息。

Formula		g/Kg
Casein	酪蛋白	195
DL-Methionine	DL-蛋氨酸	3
Sucrose	蔗糖	325.46
Corn?Starch	玉米淀粉	150
Anhydrous?Milkfat	無水乳脂	210
Cholesterol	膽固醇	12.5
Cholic?Acid	膽酸	5
Cellulose	纖維素	50
Mineral?Mix, AIN-76?(170915)	礦物混合物，AIN-76 (170915)	35
Calcium?Carbonate	碳酸鈣	4
Vitamin?Mix,?Teklad?(40060)	維生素混合物，Teklad (40060)	10
Ethoxyquin,?antioxidant	乙氧喹，抗氧化劑	0.04

Footnote

This is a modification of diet TD.88137 to increase added cholesterol to 1.25% (total in the diet is 1.3%) and add 0.5% cholic acid. TD.96121 is a version without cholic acid.

這是對飲食 TD.88137 的修改，將添加的膽固醇增加到 1.25%（飲食中的總膽固醇為 1.3%）并添加 0.5% 的膽酸。 TD.96121 是不含膽酸的版本。

Selected?Nutrient?Information1
	%?by?weight	%?kcal?from
Protein	17.3	15.4
Carbohydrate	46.9	41.9
Fat	21.2	42.6
Kcal/g 4.5 ¹?Values?are?calculated?from?ingredient?analysis?or?manufacturer?data

Key?Features

+?Purified?Diet

+?Modification?of?TD.88137

+?Cholesterol

+?Cholic?Acid

主要特征
+ 純凈飲食
+ 修改 TD.88137
+ 膽固醇
+ 膽酸

Harlan TD.01546 葉酸缺乏飼料 Folic Acid Defic. Diet成分表

發表于2022年4月7日由上海金畔生物科技有限公司

Harlan TD.01546 葉酸缺乏飼料 Folic Acid Defic. Diet成分表

Harlan代理

上海金畔生物供應Harlan TD.01546 葉酸缺乏飼料 Folic Acid Defic. Diet飼料，歡迎訪問Harlan官網或者咨詢我們獲取相關飼料報價。說明書等信息。

Harlan飼料

Formula	公式	g/Kg
L-Alanine	L-丙氨酸	3.5
L-Arginine?HCl	L-精氨酸鹽酸鹽	12.1
L-Asparagine	L-天冬酰胺	6
L-Aspartic?Acid	L-天冬氨酸	3.5
L-Cystine	L-胱氨酸	3.5
L-Glutamic?Acid	L-谷氨酸	40
Glycine	甘氨酸	23.3
L-Histidine?HCl,?monohydrate	L-組氨酸鹽酸鹽，一水合物	4.5
L-Isoleucine	L-異亮氨酸	8.2
L-Leucine	L-亮氨酸	11.1
L-Lysine?HCl	L-賴氨酸鹽酸鹽	18
L-Methionine	L-蛋氨酸	3.3
L-Phenylalanine	L-苯丙氨酸	7.5
L-Proline	L-脯氨酸	3.5
L-Serine	L-絲氨酸	3.5
L-Threonine	L-蘇氨酸	8.2
L-Tryptophan	L-色氨酸	1.8
L-Tyrosine	L-酪氨酸	5
L-Valine	L-纈氨酸	8.2
Sucrose	蔗糖	359.1787
Corn?Starch	玉米淀粉	150
Maltodextrin	麥芽糊精	150
Soybean?Oil	豆油	80
Cellulose	纖維素	30
Mineral?Mix,?AIN-93M-MX?(94049)	礦物混合物，AIN-93M-MX (94049)	35
Calcium?Phosphate,?monobasic,?monohydrate	磷酸鈣，一元，一水合物	8.2
Succinylsulfathiazole	琥珀?；前粪邕?/td>	10
Choline?Bitartrate	酒石酸膽堿	2.5
Vitamin?K,?menadione?sodium?bisulfite	維生素K、甲萘醌亞硫酸氫鈉	0.05
TBHQ,?antioxidant	TBHQ，抗氧化劑	0.02
Green?Food?Color	綠色食用色素	0.1
Niacin	煙酸	0.03
Calcium?Pantothenate	泛酸鈣	0.016
Pyridoxine?HCl	鹽酸吡哆醇	0.007
Thiamin?(81%)	硫胺素 (81%)	0.006
Riboflavin	核黃素	0.006
Folic?Acid	葉酸	0.0003
Biotin	生物素	0.0002
Vitamin?B12?(0.1%?in?mannitol)	維生素 B12（甘露醇中的 0.1%）	0.025
Vitamin?E,?DL-alpha?tocopheryl?acetate?(500?IU/g)	維生素 E，DL-α 生育酚乙酸酯 (500 IU/g)	0.15
Vitamin?A?Palmitate?(500,000?IU/g)	維生素 A 棕櫚酸酯 (500,000 IU/g)	0.008
Vitamin?D3,?cholecalciferol?(500,000?IU/g)	維生素 D3、膽鈣化醇 (500,000 IU/g)	0.002
Vitamin?K1,?phylloquinone	維生素 K1、葉綠醌	0.0008

Footnote

A modification of TD.01369, containing 3.3 g/kg methionine, 1% succinylsulfathiazole, and approx. 0.3 mg/kg folic acid. Color-coded green.

TD.01369 的改良版，含有 3.3 g/kg 蛋氨酸、1% 琥珀?；前粪邕蚝图s 0.3 毫克/公斤葉酸。顏色編碼的綠色。

Key?Features
+?Amino?Acid?Defined?Diet +?Folic?Acid +?Methionine +?Color?Coded
Selected?Nutrient?Information1
	%?by?weight	%?kcal?from
Protein CHO Fat	15.1 64.4 8.0	15.5 66.1 18.5
Kcal/g 3.9 1?Calculated?values ²?Protein?based?on?N?x 6.25 Teklad?Diets?are?designed?&?manufactured for research purposes only.

Harlan TD.08149 葉酸補充飼料Folic Acid Suppl. Diet

發表于2022年4月6日由上海金畔生物科技有限公司

Harlan TD.08149 葉酸補充飼料Folic Acid Suppl. Diet

Harlan TD.08149 葉酸補充飼料

英文名稱：Folic Acid Suppl. Diet (20, 3.3 Met., S)

Formula	g/Kg
L-Alanine	3.5
L-Arginine?HCl	12.1
L-Asparagine	6.0
L-Aspartic?Acid	3.5
L-Cystine	3.5
L-Glutamic?Acid	40.0
Glycine	23.3
L-Histidine?HCl,?monohydrate	4.5
L-Isoleucine	8.2
L-Leucine	11.1
L-Lysine?HCl	18.0
L-Methionine	3.3
L-Phenylalanine	7.5
L-Proline	3.5
L-Serine	3.5
L-Threonine	8.2
L-Tryptophan	1.8
L-Tyrosine	5.0
L-Valine	8.2
Sucrose	349.412
Corn?Starch	150.0
Maltodextrin	150.0
Soybean?Oil	80.0
Cellulose	30.0
Mineral?Mix,?AIN-93M-MX?(94049)	35.0
Calcium?Phosphate,?monobasic,?monohydrate	8.2
Succinylsulfathiazole	10.0
Vitamin?Mix,?AIN-93-VX?(94047)	10.0
Choline?Bitartrate	2.5
Vitamin?K,?menadione?sodium?bisulfite	0.05
Folic?Acid	0.018
TBHQ,?antioxidant	0.02
Pink?Food?Color	0.1

公式	克/公斤
L-丙氨酸	3.5
L-精氨酸鹽酸鹽	12.1
L-天冬酰胺	6
L-天冬氨酸	3.5
L-胱氨酸	3.5
L-谷氨酸	40
甘氨酸	23.3
L-組氨酸鹽酸鹽，一水合物	4.5
L-異亮氨酸	8.2
L-亮氨酸	11.1
L-賴氨酸鹽酸鹽	18
L-蛋氨酸	3.3
L-苯丙氨酸	7.5
L-脯氨酸	3.5
L-絲氨酸	3.5
L-蘇氨酸	8.2
L-色氨酸	1.8
L-酪氨酸	5
L-纈氨酸	8.2
蔗糖	349.412
玉米淀粉	150
麥芽糊精	150
豆油	80
纖維素	30
礦物混合物，AIN-93M-MX (94049)	35
磷酸鈣，一堿基，一水合物	8.2
琥珀?；前粪邕?/td>	10
維生素混合物，AIN-93-VX (94047)	10
膽堿酒石酸氫鹽	2.5
維生素 K、甲萘醌亞硫酸氫鈉	0.05
葉酸	0.018
TBHQ，抗氧化劑	0.02
粉紅色食物顏色	0.1

Footnote

This is a modification of TD.01369, containing 3.3 g/kg methionine, 1% succinylsulfathiazole, and approx. 20 mg/kg folic acid. Color coded pink.

腳注

這是 TD.01369 的改良版，含有 3.3 g/kg 蛋氨酸、1% 琥珀?；前粪邕蚝图s 20毫克/公斤葉酸。顏色編碼粉紅色。

+?Amino?Acid?Defined?Diet

+?Folic?Acid?Supplemented

+?Methionine?Adjusted

+?Succinylsulfathiazole

+?氨基酸?定義的飲食

+?補充葉酸

+?蛋氨酸調整

+?琥珀?；前粪邕?/td>

Selected?Nutrient?Information1

%?by?weight

%?kcal?from

Protein CHO

Fat

15.1

64.4

8.0

15.5

66.1

18.5

Kcal/g 3.9

1?Calculated?values

²?Protein?based?on?N?x 6.25

Teklad?Diets?are?designed?&?manufactured for research purposes only.

非酒精性脂肪肝NAFLD動物模型的多種飲食誘導方案

發表于2022年4月2日由上海金畔生物科技有限公司

回復

非酒精性脂肪肝NAFLD動物模型的多種飲食誘導方案

非酒精性脂肪肝疾?。∟AFLD）和酒精性肝?。ˋLD）是慢性肝病中最主要的兩種。NAFLD包括一系列病變，從肝脂肪變性到炎癥，在沒有大量飲酒的情況下，伴有或不伴有纖維化。

? 適用于研究NAFLD的動物模型有新西蘭兔、大鼠、小鼠，其中大鼠和小鼠比較常見。本文介紹幾種由不同飲食誘導的大小鼠NAFLD模型。

飲食誘導模型

相似于人類NAFLD的代謝紊亂，以營養過剩為基礎的動物模型可以模擬肝臟的組織學特征。飲食誘導的NAFLD動物模型主要基于高脂、高糖等飲食結構，這些動物模型通常包括一種或多種飲食模式組合，可誘發單純性脂肪變性和脂肪性肝炎。

1.高脂飲食：高脂飲食的脂肪供能占比通常在45-70kcal%，造模周期為8-10周，實驗動物包括C57BL/6小鼠、BALB/c小鼠、SD大鼠、Lewis大鼠、Wistar大鼠，其中大鼠更容易被高脂飲食誘導。

高脂飼料誘導大鼠構建NAFLD模型

非酒精性脂肪肝（NAFLD）包含了一系列的疾病發展階段，從一般脂肪變性進展到脂肪變性與炎癥并發的非酒精性脂肪肝炎（NASH），隨后進展為肝纖維化、肝硬化和肝癌。大多數肥胖的成年人有脂肪肝型態，他們的患病率達到30%以上，因此，NAFLD的患病率會隨著肥胖率的升高而升高。

為研究NAFLD的發病機制并找出治療方法，建立動物模型是至關重要的一步?？蒲腥藛T大多利用高脂飼料誘導大鼠建立NAFLD模型，但近幾年建立的NAFLD動物模型所采用的高脂飼料配方、建模周期均不一致，在研究方面具有不同的參考價值。本文對多種高脂飼料誘導大鼠建立NAFLD模型的方法進行總結，如有疑問，歡迎與我們的技術團隊討論。

飼養環境

環境是飼養實驗動物的必要條件之一，對于實驗動物的成活率有著很大的影響，在相關論文中對溫度和濕度幾乎都有詳細敘述，并強調了它的重要性?？紤]到NAFLD模型對環境并無特殊要求，所以科研人員一般采取了22±2℃，濕度50%-60%，12小時循環照明的普通飼養環境。

不過值得注意的是，大鼠對于環境的變化很敏感，因此要保證飼養環境各項指標的穩定。同時由于高脂飼料的緣故，大鼠的排泄物異味更重，需要及時更換墊料，飼料和飲水也需要滅菌處理，以免對實驗造成不必要的影響。

實驗大鼠的選擇

作為實驗的受體，大鼠的選擇十分重要，這不僅關系到實驗成本，也決定了建模的質量及周期。

清潔級別

對于大鼠的清潔級別選擇，綜合多篇參考文獻，即便使用普通動物，對實驗結果也沒有明顯影響，但考慮到降低大鼠死亡率和實驗成本，清潔級別越高的大鼠無疑是越好的。

種類選擇

對于實驗大鼠的選擇主要有Wistar大鼠和SD大鼠。其中SD大鼠相對發育更快，適應能力更強，更利于建模；缺點是價格較高，性格更兇猛。綜合考慮，我們認為SD大鼠仍然是更好的選擇，但如果預算較少也可以選擇前者。

性別選擇

在查閱參考的十多篇文獻中，科研人員無一例外的選擇了雄性大鼠。由于成年的雌性動物代謝存在著明顯的周期性，這可能會對實驗造成影響，所以在大部分實驗中，科研人員更傾向于選擇雄性大鼠進行實驗。

體重選擇

成年大鼠的體重可達300g，考慮到實驗時間一般為8-12周，我們認為選取5-6周，即120g-200g的大鼠比較合適。值得注意的是，大鼠之間的體重差異必須小于10%，以免引入無關的變量，對實驗結果造成干擾。

高脂飼料配方

高脂飼料的配方無疑是NAFLD建模最關鍵的因素之一，合適的高脂飼料可以實現低死亡率低，快速建立模型的目標。以下我們將對于高脂飼料的配方進行詳細探討。

豬油含量

豬油含量是高脂飼料的核心，更高的豬油含量可以快速建立NAFLD模型，但會影響大鼠食欲及死亡率；豬油含量過低則會造成建模周期長，癥狀不明顯。在《高脂血癥非酒精性脂肪肝大鼠模型的建立》中，科研人員雖然采用了42%豬油含量的高脂飼料在第8周建模成功，但個人認為并不可取，因為如此高的豬油含量會大幅度提高大鼠死亡率。我們建議使用10%豬油含量的高脂飼料，可以在12-16周得到癥狀較明顯的NAFLD模型，而且在環境適宜的情況下，通常不會有大鼠死亡。如果要縮短建模周期，可以適當提高豬油含量，但需要加入其它物質來促進吸收。

其它物質

由于高脂飼料在適口性方面不如常規飼料，會導致大鼠采食量下降，在一些實驗中甚至出現了實驗組大鼠體重低于對照組的情況。所以很多研究人員在高脂飼料中添加了膽鹽（1%），以提高大鼠食欲，促進消化吸收。同時也可以在飼料中添加膽固醇（1%-5%），以促進脂肪肝的形成，縮短建模周期。

建模周期

建模周期和許多因素相關，不能一概而論。例如高脂飼料中的豬油含量對建模周期的影響就非常大。以下我們將討論使用10%豬油含量飼料前提下所需的建模周期。

在《高脂飲食誘導肥胖與肥胖抵抗型非酒精性脂肪肝大鼠模型的建立》中，研究人員在8周停止建模，解剖大鼠，表明建模成功，但癥狀較輕，大部分大鼠處于輕度和中度肝炎。而上文提到的研究中，建模時間長達16周，但癥狀明顯，大部分大鼠處于中度和重度肝炎。綜上所述，可以證明12-16周是比較合適的建模周期，具體時間可結合需要選擇。

木棒磨牙

在《非酒精性脂肪肝動物模型的建立與思考》中，科研人員考慮到高脂飼料質地偏軟，所以為實驗組大鼠提供了木棒磨牙，結果確實提高了大鼠存活率，體重也是實驗組高于對照組的理想狀況。

剔除肥胖基因抵抗大鼠

《高脂飲食誘導肥胖與肥胖抵抗型非酒精性脂肪肝大鼠模型的建立》一文中探討了肥胖抵抗基因大鼠對于建立NAFLD模型的影響。實驗結果表明，在同樣的飼養條件下，肥胖抵抗基因大鼠的癥狀確實輕于普通大鼠，這不利于NAFLD模型的建立，所以在實驗前應當將其剔除。

總結

高脂飼料誘導大鼠建立NAFLD模型，應選擇120-200g的雄性SD大鼠，剔除肥胖抵抗基因大鼠；環境溫度應控制在22℃左右，濕度50%-60%；喂養10%豬油含量的高脂飼料，可添加膽鹽和膽固醇，在12-16周基本可以建模成功。

2.高膽固醇飲食：含1.25%膽固醇和0.5%膽酸鹽的飲食可誘導脂肪變性、炎癥和纖維化形成；

3.蛋氨酸膽堿缺乏（MCD）飲食：MCD飲食中蔗糖含量高，脂肪含量適中，但缺乏蛋氨酸和膽堿，它們是肝臟氧化和產生低密度脂蛋白(VLDL)的必要營養素；

蛋氨酸、膽堿聯合缺乏飼料(MCD)(MCD: methionine- and choline deficient diet)也稱做MCDD,是在脂肪代謝、高脂血癥的研究非酒精性脂肪肝病模型,甲基化模型腫瘤模型等疾病模型的造模中經常用到的一款飼料。

蛋氨酸膽堿缺乏模型飼料（AIN-76）

經典MCD飼料
通常我們所見到的沒有做特殊說明的MCD飼料都是經典MCD飼料。
這款飼料基于Ain-76標準，10%脂肪含量，蛋氨酸。膽堿不添加，含量基本為0。
一般多用于非酒精性脂肪肝炎(NASH)）及后期的肝纖維化、肝硬化方面的造模。
經典MCD飼料在飼喂大、小鼠時，反應相當劇烈，實驗鼠體重會持續下降，且不發生胰島素抵抗。死亡率大大增加。
這與其它方法誘導的非酒精性脂肪肝（NAFLD)大、小鼠模型有顯著的不一樣,同時也與人類典型的非酒精性脂肪肝炎(NASH)）患者情況相反，人類的非酒精性脂肪肝炎(NASH)患者不但會產生肥胖而且對胰島素抵抗。
非酒精性脂肪肝(NAFLD)(不推薦)
造模周期:2-4周
飼喂方式:自由采食
儲存方式:4攝氏度冷藏或-20攝氏度冷凍，避光
對照組:MCs (MCD同源配方,增加蛋氨酸和膽堿)
小貼士:部分MCD飼料在儲存時沒有做冷藏避光處理，會隨著時間越來越黑(避光冷藏會減緩》，這是一種稱為美拉德反應的效果，實際不影響造模效果。
非酒精性脂肪肝炎(NASH)
造模周期:5-8周
飼喂方式:自由采食
儲存方式:4攝氏度冷藏或-20攝氏度冷凍,避光
對照組:McS (MCD同源配方,增加蛋氨酸和膽堿)
小貼士:部分MCD飼料在儲存時沒有做冷藏避光處理，會隨著時間越來越黑(避光冷藏會減緩)，這是一種稱為美拉德反應的效果，實際不影響造模效果。

蛋氨酸膽堿缺乏（MCD）飼料普遍應用于脂肪代謝、高脂血癥的研究，是構建非酒精性脂肪肝模型、甲基化模型、腫瘤模型的常用飼料。

適用動物：實驗大/小鼠（C57、ICR、BALB/C、SD等常見品系）

適用周齡：3-4周離乳即可（若有特殊實驗目的，可根據實際情況選取不同周齡的大/小鼠）

產品形狀：棒狀顆粒（如有特殊需求可以提供粉料等）

儲存方式：4℃冷藏（建議）或常溫

建議采購量：小鼠按照5克/只/天計算、大鼠按照25克/只/天計算

4.膽堿缺乏性氨基酸（CDAA）飲食：與MCD飲食相似，因為它們都缺乏膽堿。不同之處在于，L-氨基酸的混合物取代了等價相應的蛋白質。

Harlan teklad-Harlan動物飼料

發表于2022年3月25日由上海金畔生物科技有限公司

Harlan teklad-Harlan動物飼料

Harlan teklad（Envigo）提供常見的高脂飼料,維生素缺乏飼料,氨基酸缺乏飼料,礦物質缺乏/調比飼料,DDC飼料,TAM飼料等，歡迎訪問Harlan teklad（Envigo）官網或者咨詢我們獲取相關飼料的報價成分使用說明等產品信息。

Harlan teklad代理

貨號	英文名稱	中文名稱
TD.88137	Adjusted calories diet(42% from fat)	調整熱量飲食（42% 來自脂肪）
TD.160153	Ketogenic 93M Diet	生酮93M飲食
TD.88137	Adjusted calories diet(42% from fat)	調整熱量飲食（42% 來自脂肪）
TD.150300	Control Diet (Crisco, CO, 0.0734 g/Kg TBHQ)	控制飲食（Crisco，CO，0.0734 g/Kg TBHQ）
TD.120528	42% Kcal/Fat Diet (Incr. Sucrose, 1.25% Chol.)	42% 大卡/脂肪飲食（增加蔗糖，1.25% 膽汁。）
TD.07797	Ketogenic Diet (6:1)	生酮飲食 (6:1)

Harlan TD.01369 葉酸控制飲食 Folic Acid Control Diet (3.3 Met., S)

發表于2022年3月25日由上海金畔生物科技有限公司

Harlan TD.01369 葉酸控制飲食 Folic Acid Control Diet (3.3 Met., S)

Formula	g/Kg
L-Alanine	3.5
L-Arginine?HCl	12.1
L-Asparagine	6.0
L-Aspartic?Acid	3.5
L-Cystine	3.5
L-Glutamic?Acid	40.0
Glycine	23.3
L-Histidine?HCl,?monohydrate	4.5
L-Isoleucine	8.2
L-Leucine	11.1
L-Lysine?HCl	18.0
L-Methionine	3.3
L-Phenylalanine	7.5
L-Proline	3.5
L-Serine	3.5
L-Threonine	8.2
L-Tryptophan	1.8
L-Tyrosine	5.0
L-Valine	8.2
Sucrose	349.53
Corn?Starch	150.0
Maltodextrin	150.0
Soybean?Oil	80.0
Cellulose	30.0
Mineral?Mix,?AIN-93M-MX?(94049)	35.0
Calcium?Phosphate,?monobasic,?monohydrate	8.2
Succinylsulfathiazole	10.0
Vitamin?Mix,?AIN-93-VX?(94047)	10.0
Choline?Bitartrate	2.5
Vitamin?K,?menadione?sodium?bisulfite	0.05
TBHQ,?antioxidant	0.02

配方克/公斤
L-丙氨酸 3.5
L-精氨酸鹽酸鹽 12.1
L-天冬酰胺 6.0
L-天冬氨酸 3.5
L-胱氨酸 3.5
L-谷氨酸 40.0
甘氨酸 23.3
L-組氨酸鹽酸鹽，一水合物 4.5
L-異亮氨酸 8.2
L-亮氨酸 11.1
L-賴氨酸鹽酸鹽 18.0
L-蛋氨酸 3.3
L-苯丙氨酸 7.5
L-脯氨酸 3.5
L-絲氨酸 3.5
L-蘇氨酸 8.2
L-色氨酸 1.8
L-酪氨酸 5.0
L-纈氨酸 8.2
蔗糖 349.53
玉米淀粉 150.0
麥芽糊精 150.0
豆油 80.0
纖維素 30.0
礦物混合物，AIN-93M-MX (94049) 35.0
一水合磷酸二氫鈣 8.2
琥珀?；前粪邕?10.0
維生素混合物，AIN-93-VX (94047) 10.0
膽堿酒石酸氫鹽 2.5
維生素 K、甲萘醌亞硫酸氫鈉 0.05
TBHQ，抗氧劑 0.02

Footnote

This is a modification of TD.99366, containing 3.3 g/kg methionine, 1% succinylsulfathiazole, and approx. 2 mg/kg folic acid.

這是 TD.99366 的改良版，含有 3.3 g/kg 蛋氨酸、1% 琥珀?；前粪邕蚝图s 2毫克/公斤葉酸。

Key?Features

+?Amino?Acid?Defined?Diet

+?Folic?Acid

+?Methionine

+?Succinylsulfathiazole

主要特征
+ 氨基酸定義飲食
+ 葉酸
+ 蛋氨酸
+ 琥珀?；前粪邕?/p>

Harlan 1.5% 含磷飲食1.5% Phosphorus Diet TD.03625

發表于2022年3月25日由上海金畔生物科技有限公司

Harlan 1.5% 含磷飲食1.5% Phosphorus Diet TD.03625

Egg White Solids, spray-dried	200
L-Tryptophan	1.6
Corn Starch	150
Sucrose	512.767
Corn Oil	50
Vitamin Mix, Teklad (40060)	10
Biotin	0.004
Mineral Mix, Ca-P Deficient (79055)	13.369
Calcium Phosphate, monobasic, monohydrate	36.73
Sodium Phosphate, monobasic, monohydrate	12.85
Potassium Phosphate, monobasic	12.68

蛋清固體，噴霧干燥 200
L-色氨酸 1.6
玉米淀粉 150
蔗糖 512.767
玉米油 50
維生素混合物，Teklad (40060) 10
生物素 0.004
礦物質混合物，缺乏鈣磷 (79055) 13.369
一水合磷酸二氫鈣 36.73
一水合磷酸二氫鈉 12.85
磷酸二氫鉀 12.68

Footnote

This is a modification of TD.80235 (P defic.) to add 1.5% P from a combination of calcium, sodium, and potassium phosphate salts. Approx.?levels of Ca – 0.6%, Na – 0.6%, and K – 1%.

這是對 TD.80235 (P defic.) 的修改，從磷酸鈣鹽、鈉鹽和磷酸鉀鹽的組合中添加 1.5% P。大約。 Ca – 0.6%、Na – 0.6% 和 K – 1% 的含量。

Selected?Nutrient?Information1
%?by?weight	%?kcal?from
Protein 16.3 Carbohydrate 65.2 Fat 5.0	17.5 70.3 12.1
Kcal/g 3.7 ¹?Values?are?calculated?from?ingredient?analysis?or?manufacturer?data

Harlan 嚙齒動物飼料 Rodent diets

發表于2022年1月14日由上海金畔生物科技有限公司

Harlan 嚙齒動物飼料 Rodent diets

Harlan Teklad動物飼料
歡迎新老客戶訪問Harlan官網或者咨詢上海金畔生物獲取更多詳細資料。

Rodent diets

As a researcher, you want to have as much control as possible over what goes into your study animals. At Envigo, we understand that. That is why we developed Teklad Global Rodent Diets^? .

Teklad Global Rodent Diets^? are a special integrated range of vegetarian laboratory rodent diets developed to be nutritionally complete for various life stages from breeding through long-term maintenance. Global rodent laboratory diets contain levels of protein, energy, vitamins and minerals that are more appropriate to the needs of modern biomedical research studies.

作為一名研究人員，您希望盡可能多地控制進入您的研究動物的內容。在 Envigo，我們明白這一點。這就是我們開發 Teklad Global Rodent Diets? 的原因。

Teklad Global Rodent Diets? 是一種特殊的綜合素食實驗室嚙齒動物飲食系列，旨在為從繁殖到長期維持的各個生命階段提供營養完整的食物。全球嚙齒動物實驗室飲食含有更適合現代生物醫學研究需求的蛋白質、能量、維生素和礦物質水平。

order today

18301939375

Furthermore, in global rodent diets particular attention has been placed on avoiding, as far as practical, ingredients that are reported to have adverse confounding effects on experimental results. This has resulted in a range of Teklad rodent laboratory diets that contain:

No fish meal
No meat meals or meat by-products
No alfalfa meal
No soybean meal or reduced levels
No animal fat

By excluding animal by-products, the presence of nitrosamines (a potential carcinogen) is avoided. Exclusion of alfalfa meal reduces chlorophyll, improving optical imaging clarity. Reduction or removal of soybean meal, together with elimination of alfalfa meal, minimizes levels of naturally-occurring phytoestrogens. Phytoestrogens interact with endogenous estrogens and potentially can affect studies in many research areas. Read more about this in these pieces:

Dietary phytoestrogens, a source of research variation
Ask a nutritionist series: Impact of phytoestrogens on research, Volume 1
Phytoestrogens limit translation of preclinical results to clinical outcomes
Ask a nutritionist series: Impact of phytoestrogens on research, Volume 2

通過排除動物副產品，避免了亞硝胺（一種潛在的致癌物質）的存在。苜蓿粉的排除減少了葉綠素，提高了光學成像的清晰度。減少或去除豆粕，同時去除苜蓿粉，可以最大限度地減少天然植物雌激素的含量。植物雌激素與內源性雌激素相互作用，并可能影響許多研究領域的研究。在這些文章中閱讀更多相關信息：

膳食植物雌激素，研究變化的來源
詢問營養師系列：植物雌激素對研究的影響，第 1 卷

植物雌激素限制了將臨床前結果轉化為臨床結果
詢問營養師系列：植物雌激素對研究的影響，第 2 卷

Products

Teklad rodent diets	Non-autoclavable form	Autoclavable	Irradiated
Teklad global 14% protein	2014, 2014C		2914
Teklad global 16% protein	2016, 2016C		2916
Teklad global 18% protein	2018, 2018C	2018S, 2018SX	2918
Teklad global 19% protein extruded	2019	2019S	2919
Teklad global soy protein-free extruded	2020X	2020SX	2920X
Traditional diets	see more	see more	see more

Not all products are stocked locally; extended lead time and additional fees may apply.
Many diets are available in certified format designated by a “C” following the product code. When diets are certified a representative sample is tested for a panel of contaminants. If not stocked as certified, certification can be made available upon request. Minimum order size and additional charges may apply.

Traditional rodent diets were formulated decades ago based on understanding of rodent nutrition, ingredients, and diet manufacturing at the time. While traditional diets will supply the known nutrient needs of your laboratory animals, we recommend you consider the use of a diet from our newer global diet line for your modern research needs.

Teklad Global Rodent Diets^? are modern formulas designed to reduce research variables. Specifically, these diets contain more appropriate nutrient levels, and limit or exclude ingredients that are reported to have effects on a wide variety of research endpoints.

Lower, more appropriate protein levels can improve survival and reduce morbidity
Vegetarian with no nitrosamines (a potential carcinogen)
Formulated to exclude alfalfa meal, greatly improving fluorescent imaging clarity
Formulated to exclude or lower soybean meal, thus minimizing the presence of isoflavones, the primary type of phytoestrogen found in lab animal diets
Extruded rodent diets dramatically reduce clumping and hardness after autoclaving (2018SX , 2019S , 2020SX ), and in general result in less diet waste and cleaner cages

Teklad rodent diets are natural-ingredient diets specifically formulated to provide the proper balance of all known nutrients considered essential for the growth, maintenance, and reproduction of rats, mice, gerbils and hamsters. These diets conform to the nutrient requirements for rodents established by the National Research Council (1995).

Teklad rodent diets provide uniform nutrition. They are fixed-formula diets designed to minimize the nutrient variances which otherwise could occur if the ingredient composition of a diet were altered from one batch to the next.

Protein is supplied primarily by plant sources. Supplemental amino acids are added to provide the proper amount and balance of essential amino acids. All rodent diets are fortified with vitamins and minerals to help support the regulation of body fluids and the proper functioning of body systems to ensure the adequate growth, maintenance, and reproduction of research rodents. Autoclavable diets are supplemented with additional vitamins to compensate for losses that occur during autoclaving. Since our diets are nutritionally complete and balanced, it is not necessary to add dietary supplements.

There is no definitive point where one is able to predict when a specific diet will spoil or become deficient in one or more nutrients. The common guideline of a six month shelf life is based on longstanding practice in North America. In Europe and Asia, differences in local practices and regulatory oversight have led to Teklad standard natural ingredient diets being routinely used out to nine months and sometimes 12 months post-manufacture. This practical experience, along with literature support and vitamin testing over time, gives us confidence that these diets continue to support animal health and study integrity out to at least nine months post-manufacture. Please refer to your institution for guidance if you are unsure of local policies.

Recommended storage conditions:

Cool and dry; at or below 70 degrees fahrenheit with humidity ideally below 50%, but up to 65% is acceptable
Clean and free of pests
In original packaging or in a container that prevents continuous exposure to light and minimal exposure to air

Standard natural ingredient rodent diets are complete diets for rats, mice, gerbils and hamsters, that should be allowed ad libitum access to the diet. Fresh water should be available at all times.

并非所有產品都在當地備貨；可能需要延長交貨時間和額外費用。
許多飲食都可以通過產品代碼后面的“C”指定的認證格式獲得。當日糧獲得認證時，會針對一組污染物對代表性樣本進行測試。如果庫存沒有經過認證，可根據要求提供認證。最小訂單量和額外費用可能適用。

傳統的嚙齒動物飲食是幾十年前基于對當時嚙齒動物營養、成分和飲食制造的了解而制定的。雖然傳統飲食將滿足您的實驗動物已知的營養需求，但我們建議您考慮使用我們更新的全球飲食系列中的飲食來滿足您的現代研究需求。

Teklad Global Rodent Diets? 是現代配方，旨在減少研究變量。具體來說，這些飲食含有更適當的營養水平，并限制或排除據報道對各種研究終點有影響的成分。

更低、更合適的蛋白質水平可以提高生存率并降低發病率
不含亞硝胺（潛在致癌物）的素食主義者
配方可排除苜蓿粉，大大提高熒光成像清晰度
配方可排除或減少豆粕，從而最大限度地減少異黃酮的存在，異黃酮是實驗室動物飲食中發現的主要植物雌激素類型
膨化嚙齒動物飼料可顯著減少高壓滅菌后的結塊和硬度（2018SX、2019S、2020SX），通常會減少飼料浪費和清潔籠子
Teklad 嚙齒動物飼料是一種天然成分的飼料，專門配制用于提供所有已知營養素的適當平衡，這些營養素被認為對大鼠、小鼠、沙鼠和倉鼠的生長、維持和繁殖至關重要。這些飲食符合國家研究委員會 (1995) 制定的嚙齒動物的營養需求。

Teklad 嚙齒動物飲食提供均勻的營養。它們是固定配方的飲食，旨在最大限度地減少營養差異，否則如果飲食的成分組成從一批更改為下一批，則可能發生這種情況。

蛋白質主要由植物來源提供。添加補充氨基酸以提供適量和平衡的必需氨基酸。所有嚙齒動物的飲食都含有維生素和礦物質，以幫助支持體液的調節和身體系統的正常運作，以確保研究嚙齒動物的充分生長、維持和繁殖?？筛邏簻缇娘嬍逞a充了額外的維生素，以補償高壓滅菌期間發生的損失。由于我們的飲食營養完整且均衡，因此沒有必要添加膳食補充劑。

沒有明確的點可以預測特定飲食何時會變質或缺乏一種或多種營養素。六個月保質期的通用準則基于北美的長期實踐。在歐洲和亞洲，當地實踐和監管監督的差異導致 Teklad 標準天然成分飲食通常在生產后 9 個月，有時甚至 12 個月內使用。這種實踐經驗，加上文獻支持和維生素測試，讓我們相信這些飲食在生產后至少 9 個月內繼續支持動物健康和研究完整性。如果您不確定當地政策，請咨詢您的機構以獲得指導。

推薦的儲存條件：

涼爽干燥；在華氏 70 度或以下，濕度理想地低于 50%，但最高 65% 是可以接受的
干凈無害蟲
在原包裝或容器中，以防止持續暴露在光線下并盡量減少暴露在空氣中
標準的天然成分嚙齒動物飲食是大鼠、小鼠、沙鼠和倉鼠的完整飲食，應允許隨意獲取飲食。淡水應隨時可用。

Technical resources??技術文章

Live chatLive chat ?

圖片僅供參考,請以實物為準。
若本網站沒有及時更新，請大家諒解！
正文中列出的所有試劑只能用于測試或研究，不能作為”藥品”，”食品”，”家庭用品”等使用。
我司所銷售的化學試劑、原料等所有產品（包括但不限于抗生素類、蛋白質類、試劑盒類產品等）僅限用于科學研究用途，不得作用于人體。

Harlan 定制飼料成分 Custom diet ingredients

發表于2022年1月14日由上海金畔生物科技有限公司

Harlan 定制飼料成分 Custom diet ingredients

Harlan Teklad動物飼料
歡迎新老客戶訪問Harlan官網或者咨詢上海金畔生物獲取更多詳細資料。

Diet ingredients

You may want to prepare your own diet at your research site or facility, and if that is the case, you depend on the ingredients being of the highest quality. Envigo can provide you with many of the same ingredients used to produce our advanced Teklad custom research diets so you can develop your own formula. And as always, our nutritional experts are here to help you make the best decisions for your ingredients and ultimate formulation.

您可能想在您的研究地點或設施中準備自己的飲食，如果是這種情況，您取決于最高質量的成分。 Envigo 可以為您提供許多用于生產我們先進的 Teklad 定制研究飲食的相同成分，因此您可以開發自己的配方。與往常一樣，我們的營養專家會幫助您為您的成分和最終配方做出最佳決策。

Our ingredients

We offer several ingredients for individual sale so that you can develop your own blends.

我們的成分

我們提供多種成分供單獨銷售，以便您開發自己的混合物。

“VFT” casein

“Vitamin-free” test casein (alcohol-extracted) is best suited for purified test diet formulas where fat or vitamin content needs to be precisely controlled
The reduced levels of many vitamins in VFT casein make it the preferred protein source for many vitamin studies. Thus, the nickname “Vitamin-free” was given to the extracted casein many years ago

Vitamin mixes

The common vitamin mixes shown below use sucrose or corn starch as a carrier
Customized vitamin mixes may use cellulose if a non-nutritive carrier is necessary
Vitamin mixes are formulated with a diet inclusion rate in mind. Using more or less than recommended could impair the health of your laboratory animal
For those preparing their own diets, vitamin premixes are an efficient way to add vitamins to a diet
Vitamin mixes should be stored at 4 degrees celsius or lower upon receipt. Storage at –20 degrees celsius is recommended if the mix will be used beyond 1 month
Minimum order is 500 g for both stock and custom vitamin mixes
Our available vitamin mixes are commonly used in diets, and are available from stock
Vitamin mixes are shipped within a few days of order

“VFT”酪蛋白

“無維生素”測試酪蛋白（酒精提?。┳钸m合需要精確控制脂肪或維生素含量的純化測試飲食配方
VFT 酪蛋白中許多維生素的含量降低，使其成為許多維生素研究的首選蛋白質來源。因此，多年前提取的酪蛋白被稱為“無維生素”
維生素混合物

下面顯示的常見維生素混合物使用蔗糖或玉米淀粉作為載體
如果需要非營養載體，定制的維生素混合物可以使用纖維素
維生素混合物的配制考慮了飲食的包含率。使用多于或少于推薦量可能會損害您的實驗動物的健康
對于那些準備自己的飲食的人來說，維生素預混料是一種在飲食中添加維生素的有效方法
維生素混合物在收到時應儲存在 4 攝氏度或更低溫度下。如果混合物的使用時間超過 1 個月，建議在 –20 攝氏度下儲存
庫存和定制維生素混合物的最低訂購量為 500 克
我們提供的維生素混合物常用于飲食，并且有現貨供應
維生素混合物在訂購后幾天內發貨

Our Formula examples

CA.40060 ???? Teklad vitamin mix
CA.40077 ???? AIN-76A vitamin mix
TD.94047 ???? AIN-93 vitamin mix

我們的公式示例

CA.40060 Teklad 維生素混合物
CA.40077 AIN-76A 維生素混合物
TD.94047 AIN-93 維生素混合物

Mineral mixes

The common mineral mixes shown below as examples use sucrose as a carrier
Customized mineral mixes could use cellulose or no carrier if a non-nutritive carrier is necessary
Our mineral mixes are formulated with a diet inclusion rate in mind. Using more or less than recommended could impair the health of the animal
For those preparing their own diets, mineral premixes are an efficient way to add minerals to a diet
Mineral mixes should be stored in a dry cool environment and used within a year of receipt. The minimum order is 500 g for both stock and custom mineral mixes
These mineral mixes are commonly used in diets, and are available from stock
Our mineral mixes are shipped within a few days of order

礦物混合物

下面作為示例顯示的常見礦物混合物使用蔗糖作為載體
如果需要非營養載體，定制的礦物混合物可以使用纖維素或不使用載體
我們的礦物質混合物在配制時考慮了飲食包含率。使用多于或少于推薦量可能會損害動物的健康
對于那些準備自己的飲食的人來說，礦物質預混料是一種在飲食中添加礦物質的有效方法
礦物混合物應儲存在干燥涼爽的環境中，并在收到后一年內使用。庫存和定制礦物混合物的最低訂購量為 500 克
這些礦物質混合物常用于飲食，并且有現貨供應
我們的礦物混合物在訂購后的幾天內發貨

Formula examples:

CA.170915 ??? AIN-76 mineral mix
TD.94046 ????? AIN-93G mineral mix
TD.94049 ????? AIN-93M mineral mix

Other mixes (may or may not be available from stock):

TD.83171 ???? Vitamin mix without A, D, E, choline
TD.81062 ???? Iron deficient mineral mix based on AIN-76A
TD.79055 ???? Calcium?and phosphorus deficient mineral mixed based?on AIN-76
TD.98057 ???? Calcium?and phosphorus deficient mineral mixed based on AIN-93
CA.170760 ?? Rogers-Harper mineral mix

order today

18301939375

上海金畔生物科技有限公司

Live chatLive chat ?

Harlan圖片僅供參考,請以實物為準。

若本網站沒有及時更新，請大家諒解！
正文中列出的所有試劑只能用于測試或研究，不能作為”藥品”，”食品”，”家庭用品”等使用。
我司所銷售的化學試劑、原料等所有產品（包括但不限于抗生素類、蛋白質類、試劑盒類產品等）僅限用于科學研究用途，不得作用于人體。

Harlan 維生素調整飲食Vitamin adjusted diets

發表于2022年1月13日由上海金畔生物科技有限公司

Harlan 維生素調整飲食Vitamin adjusted diets

Harlan Teklad動物飼料
歡迎新老客戶訪問Harlan官網或者咨詢上海金畔生物獲取更多詳細資料。

Vitamin adjusted diets

A few vitamin adjusted formulas are shown below. Please contact us for additional formulas of this nature or for more information about altering the vitamin profile of a diet, and please read below to learn more.

一些維生素調整配方如下所示。請聯系我們以獲取此類性質的其他配方或有關改變飲食中維生素成分的更多信息，請閱讀以下內容以了解更多信息。

Formula Examples:

TD.86143 Vitamin A deficient diet
TD.89123 Vitamin? D deficient diet
TD.95247 Folic acid deficient diet

order today

18301939375

上海金畔生物科技有限公司

Most vitamin deficient diets use vitamin-free test casein (VFT casein) as the protein source. VFT casein is casein that has been extracted with denatured alcohol to reduce the fat content from about 1% to 0.1%. In the process the content of a number of vitamins (present at low or trace levels in casein) is further reduced, making this an ideal protein source for these types of diets. Envigo produces our own VFT in-house, and we also offer this as an ingredient for those mixing their own diets.

From this vitamin deficient base, various levels of vitamins can be added back. Many formulas adjust multiple vitamins, and other nutrients.

If use of an intact protein source is not advisable, an amino acid defined diet can be used.

大多數缺乏維生素的飲食使用不含維生素的測試酪蛋白（VFT 酪蛋白）作為蛋白質來源。 VFT酪蛋白是用變性酒精提取的酪蛋白，將脂肪含量從1%左右降低到0.1%。在此過程中，多種維生素（酪蛋白中含量較低或微量）的含量進一步降低，使其成為這類飲食的理想蛋白質來源。 Envigo 在內部生產我們自己的 VFT，我們還為那些混合他們自己的飲食的人提供這種成分。

從這種維生素缺乏的基礎上，可以補充各種水平的維生素。許多配方調整多種維生素和其他營養素。

如果不建議使用完整的蛋白質來源，則可以使用氨基酸確定的飲食。

聯系我們獲取其他配方示例或討論您的特定需求。

Live chatLive chat ?

Harlan 礦物質調整飲食Mineral adjusted diets

發表于2022年1月13日由上海金畔生物科技有限公司

Harlan 礦物質調整飲食Mineral adjusted diets

Harlan Teklad動物飼料
歡迎新老客戶訪問Harlan官網或者咨詢上海金畔生物獲取更多詳細資料。

Mineral adjusted diets

A few mineral adjusted formulas are shown below. Please contact us for additional formulas of this nature or for more information about altering the mineral profile of a diet. Read below for more information.

一些礦物質調整配方如下所示。請聯系我們以獲取此類性質的其他配方或有關改變飲食中礦物質成分的更多信息。閱讀下文了解更多信息。

Formula examples:

TD.80396 ????? Iron deficient diet
TD.85419 ????? Zinc deficient diet
TD.88239 ????? Potassium deficient diet
TD.95027 ????? Low calcium diet (0.4% P)
TD.96348 ????? Diet (20% lactose, 2% Ca, 1.25% P)
TD.92163 ????? Selenium deficient diet
TD.95125 ????? Iodine deficient (0.15% PTU)

order today

18301939375

上海金畔生物科技有限公司

Unique protein ingredients may be necessary to minimize background levels of certain minerals. Other steps taken to minimize background mineral contamination include the use of reagent grade minerals when necessary or omission of certain ingredients. Specialized mineral premixes may be used in these diets, or minerals are added individually to the diet in order to control the level of specific minerals.

With the exception of sodium, it is necessary to use refined ingredients to achieve the lowest possible levels of minerals in a diet.

From this mineral deficient base, various levels of minerals can be added back. Some formulas adjust multiple minerals, and other related nutrients.

可能需要獨特的蛋白質成分來最小化某些礦物質的背景水平。為盡量減少背景礦物質污染而采取的其他措施包括在必要時使用試劑級礦物質或省略某些成分。這些飲食中可以使用專門的礦物質預混料，或者將礦物質單獨添加到飲食中以控制特定礦物質的水平。

除鈉外，有必要使用精制成分以使飲食中的礦物質含量盡可能低。

從這種礦物質缺乏的基礎上，可以補充各種水平的礦物質。一些配方調整多種礦物質和其他相關營養素。

聯系我們討論以上所列礦物以及此處未列出的礦物的其他可能性。

Live chatLive chat ?

圖片僅供參考,請以實物為準。

Harlan蛋白質調整飲食 Protein adjusted diets

發表于2022年1月13日由上海金畔生物科技有限公司

Harlan蛋白質調整飲食 Protein adjusted diets

Harlan Teklad動物飼料
歡迎新老客戶訪問Harlan官網或者咨詢上海金畔生物獲取更多詳細資料。

Protein adjusted diets

A few protein adjusted formulas are shown below. Please contact us for additional formulas of this nature or for more information about altering the protein type or content of a diet.

一些蛋白質調整公式如下所示。請聯系我們以獲取此類性質的其他配方或有關改變飲食中蛋白質類型或含量的更多信息。

Formula examples:

TD.93328 “Protein free” diet
TD.90016 6% Protein diet
TD.91352 20% Protein diet
TD.90018 40% Protein diet

order today

18301939375

上海金畔生物科技有限公司

These diet examples are from a series of diets with the following features: casein-based, isocaloric (3.8 kcal/g) and matched for fat (5.5%), calcium (0.7%), and phosphorus (0.54%). There are other diets available from this series of diets, as well as other unique protein adjusted diets.

Casein is the most widely used refined protein source, but it is also possible to use other refined protein sources, such as “vitamin-free” test casein (alcohol extracted), lactalbumin, isolated soy protein, and egg white solids. These particular protein sources are usually used to achieve a specific research objective, rather than for routine use. For instance, vitamin-free test casein is most appropriately used in vitamin deficient diets, and egg white solids is most suitable for zinc or biotin deficient diets.

Adjusted protein diets usually range from “protein-free” (trace) to 60% protein, depending on the research.

這些飲食示例來自一系列具有以下特點的飲食：以酪蛋白為基礎、等熱量 (3.8 kcal/g) 并與脂肪 (5.5%)、鈣 (0.7%) 和磷 (0.54%) 相匹配。這一系列飲食中還有其他飲食可供選擇，以及其他獨特的蛋白質調整飲食。

酪蛋白是最廣泛使用的精制蛋白質來源，但也可以使用其他精制蛋白質來源，例如“無維生素”測試酪蛋白（酒精提?。?、乳清蛋白、大豆分離蛋白和蛋清固體。這些特定的蛋白質來源通常用于實現特定的研究目標，而不是常規使用。例如，不含維生素的測試酪蛋白最適合用于缺乏維生素的飲食，而蛋清固體最適合用于缺乏鋅或生物素的飲食。

調整后的蛋白質飲食通常范圍從“無蛋白質”（微量）到 60% 的蛋白質，具體取決于研究。

Live chatLive chat ?

Teklad diet selector

圖片僅供參考,請以實物為準。

Harlan 氨基酸定制飼料 Amino acid defined

發表于2022年1月13日由上海金畔生物科技有限公司

Harlan 氨基酸定制飼料 Amino acid defined

Harlan Teklad動物飼料
歡迎新老客戶訪問Harlan官網或者咨詢上海金畔生物獲取更多詳細資料。

Amino acid defined

A few amino acid defined formulas are shown below. This type of diet is used when a diet needs to be deficient in one or more amino acids, or adjustments need to be made to specific amino acid levels.

一些氨基酸定義的公式如下所示。當飲食需要缺乏一種或多種氨基酸或需要對特定氨基酸水平進行調整時，使用這種類型的飲食。

Formula examples:

TD.99366 ????? Amino acid diet
TD.90262 ????? Methionine?and choline deficient diet
TD.00434 ????? Folic acid deficient diet
TD.99386 ????? No lysine AA diet

order today

18301939375

上海金畔生物科技有限公司

Sometimes this type of diet is used to further reduce the background level of certain vitamins that are found in protein sources such as casein or vitamin-free (alcohol extracted) casein. Some folic acid deficient diets utilize this approach.

The amino acid profile for most amino acid defined diets is based on work by Harper and Rogers (J. Nutr. (1965) 87:267-276) and is not representative of the amino acid profile of an intact protein source such as casein. Other amino acid profiles can be specified. When an amino acid(s) is removed or reduced, isonitrogenous adjustment can be made if required.

有時，這種類型的飲食用于進一步降低蛋白質來源中某些維生素的背景水平，例如酪蛋白或無維生素（酒精提?。├业鞍?。一些缺乏葉酸的飲食采用這種方法。

大多數氨基酸確定飲食的氨基酸譜基于 Harper 和 Rogers 的工作 (J. Nutr. (1965) 87:267-276)，并不代表完整蛋白質來源（如酪蛋白）的氨基酸譜。可以指定其他氨基酸譜。當一個或多個氨基酸被去除或減少時，如果需要，可以進行等氮調節。

Live chatLive chat ?

Harlan脂肪/脂質調整飼料 Fat/lipid adjusted

發表于2022年1月13日由上海金畔生物科技有限公司

Harlan脂肪/脂質調整飼料 Fat/lipid adjusted

Harlan Teklad動物飼料
歡迎新老客戶訪問Harlan官網或者咨詢上海金畔生物獲取更多詳細資料。

Fat/lipid adjusted

There are numerous possibilities in adjusting both the level and source of fat in a diet. If your primary interest is inducing atherosclerosis or obesity, additional information can be found on our Teklad atherogenic and diet induced obesity (DIO) diet pages. Depending on your research goals and level of desired fat supplementation, fats can be added to a standard natural ingredient diet or a purified diet.

Purified ingredients are refined to the point of chemical simplicity and allow for extreme fat levels such as no fat diet TD.03314 or very high fat ketogenic diet TD.96355 . Unless adjustments are made to the level of fiber, diets with high fat levels will have a greater caloric density since fat contains 9 kcal/g versus 4 kcal/g for carbohydrate or protein. Animals are good at regulating caloric intake, therefore it is common to adjust other nutrients (vitamins, minerals, protein) relative to energy content to account for differences in feed intake.

調整飲食中脂肪的水平和來源有很多可能性。如果您的主要興趣是誘發動脈粥樣硬化或肥胖，可以在我們的 Teklad 致動脈粥樣硬化和飲食誘導肥胖 (DIO) 飲食頁面上找到更多信息。根據您的研究目標和所需的脂肪補充水平，可以將脂肪添加到標準天然成分飲食或純化飲食中。

純化的成分經過精制，達到化學簡單的程度，并允許極高的脂肪水平，例如無脂肪飲食 TD.03314 或非常高脂肪的生酮飲食 TD.96355。除非對纖維水平進行調整，否則高脂肪飲食的熱量密度會更高，因為脂肪含有 9 kcal/g，而碳水化合物或蛋白質為 4 kcal/g。動物擅長調節熱量攝入，因此通常會根據能量含量調整其他營養素（維生素、礦物質、蛋白質），以解決采食量的差異。

order today

18301939375

上海金畔生物科技有限公司

Specific fats can be utilized as in this essential fatty acid deficient diet TD.84224 or to provide high levels of omega-3 polyunsaturated fat from flaxseed or fish oil. Normally, 1-2% fat from plant sources like corn or soybean oil will supply sufficient levels of essential fatty acids. Additional fat provides energy and can influence palatability due to effects on taste and texture of the diet.

Mixtures of fats can be used to mimic a Western or Mediterranean diet fatty acid profile. Contact us for a table comparing the fatty acid composition of several stocked fat sources (solid fats, oils).

We also commonly work with customer supplied oils and purified fatty acids such as DHA and EPA. If you prefer to mix fat sources in your lab, ask about a basal mix. Basal mixes are supplied in powder form and often allow you to use one diet mix for creating several different diets.

If fat stability is a concern, contact a nutritionist to discuss options for diet drying, vacuum packaging, and synthetic antioxidant supplementation.

特定脂肪可用于這種缺乏必需脂肪酸的飲食 TD.84224，或從亞麻籽或魚油中提供高水平的 omega-3 多不飽和脂肪。通常，來自植物來源（如玉米或大豆油）的 1-2% 脂肪將提供足夠水平的必需脂肪酸。額外的脂肪提供能量，并且由于對飲食的味道和質地的影響而影響適口性。

脂肪混合物可用于模擬西方或地中海飲食的脂肪酸分布。請聯系我們獲取比較幾種庫存脂肪來源（固體脂肪、油）的脂肪酸組成的表格。

我們通常還使用客戶提供的油和純化脂肪酸，例如 DHA 和 EPA。如果您更喜歡在實驗室中混合脂肪來源，請詢問基礎混合物?；A混合物以粉末形式提供，通常允許您使用一種飲食混合物來創建幾種不同的飲食。

如果擔心脂肪穩定性，請聯系營養師討論飲食干燥、真空包裝和合成抗氧化劑補充劑的選擇。

Example diets mentioned above

TD.03314 62% sucrose diet (no fat)
TD.96355 Ketogenic diet
TD.84224 Essential fatty acid deficient diet

Live chatLive chat ?

圖片僅供參考,請以實物為準。

Harlan致動脈粥樣硬化飼料 Atherogenic

發表于2022年1月13日由上海金畔生物科技有限公司

Harlan致動脈粥樣硬化飼料 Atherogenic

Harlan Teklad動物飼料
歡迎新老客戶訪問Harlan官網或者咨詢上海金畔生物獲取更多詳細資料。

Atherogenic

Diet can be a useful tool to induce or accelerate atherosclerosis in laboratory animal models. Key dietary features used to induce atherosclerosis in rodents vary depending on the research model, desired endpoint, and length of feeding. While formulations of atherogenic diets continue to evolve, the options that are well-described in the literature are summarized below. For more information on each diet option and literature references see the expandable tabs following the diet table.

在實驗動物模型中，飲食可以成為誘導或加速動脈粥樣硬化的有用工具。用于誘發嚙齒動物動脈粥樣硬化的主要飲食特征因研究模型、所需終點和喂養時間長短而異。雖然致動脈粥樣硬化飲食的配方不斷發展，但文獻中充分描述的選項總結如下。有關每種飲食選擇和文獻參考的更多信息，請參閱飲食表后面的可擴展選項卡。

order today

18301939375

上海金畔生物科技有限公司

Research use	Key dietary features	Examples
“Western” purified atherogenic diet
Accelerated hypercholesterolemia and plaque formation in genetically modified models such as Apoe and Ldlr deficient mice. Used for diet induced obesity in a variety of rodent models.	High fat diet (20 – 23% by weight; 40 – 45% kcal from fat) Saturated fatty acids (SFA >60% of total fatty acids) Milkfat/butterfat Sucrose (34% by weight) Cholesterol (0.2% total)	TD.88137 TD.10885
“Western” purified atherogenic diet with added cholesterol and cholate source*
Induce hypercholesterolemia and mild atherosclerosis (foam cells, fatty streaks) in primarily wild type mice and rats. Will not promote obesity.	High fat diet (15 – 20% by weight;?34 – 45% kcal from fat) Saturated fatty acids (SFA >55% of total fatty acids) Milkfat/butterfat, cocoa butter Sucrose (30-50% by weight) Cholesterol (1 – 1.25%) Cholate Source (0.5%)*	TD.02028 TD.09237
Hybrid high fat diets with added cholesterol and cholate source*
Induce hypercholesterolemia and mild atherosclerosis (foam cells, fatty streaks) in primarily wild type mice and rats. Will not promote obesity. Also used for lithogenic (gallstone) rodent studies.	75% rodent breeder diet; 25% purified ingredients High fat (~15% by weight; 37% kcal from fat) Saturated fatty acids (SFA >45% of total fatty acids) Cholesterol (1.25%) Cholate source (0.5%)*	TD.88051 TD.90221
Standard diets with added cholesterol
Induce hypercholesterolemia in genetically modified and wild type models without promoting obesity.	Standard, grain-based rodent diet Minimal/moderate phytoestrogen diets recommended Cholesterol (1 – 4%)	TD.120097 TD.07841 TD.01383

*膽酸鈉或膽酸有助于膽固醇和脂肪的吸收，并通過膽汁酸合成減少膽固醇的排放。但是，如果您的研究不需要包含膽酸鹽來源，則可以使用不含膽酸鹽的飲食。

“Western” purified atherogenic diet

“Western” style diets are fed to genetically-modified cardiovascular models, such as Apoe and Ldlr deficient mice, to accelerate and enhance hypercholesterolemia and plaque formation and to elicit phenotypes commonly associated with metabolic syndrome. Within the atherogenic literature, a “Western” diet typically is described as a purified rodent diet with 20-23% milkfat/butterfat, 0.2% total cholesterol, and 34% sucrose by weight. TD.88137 ?is an example of a “Western” style diet that was originally designed to characterize and enhance atherosclerosis development in a newly generated Apoe-deficient mouse model. Contact us for more information about “Western” style diets, modifications, or possible control diets.

“西方”純化的致動脈粥樣硬化飲食

“西式”飲食被喂給轉基因心血管模型，例如 Apoe 和 Ldlr 缺陷小鼠，以加速和增強高膽固醇血癥和斑塊形成，并引發通常與代謝綜合征相關的表型。在致動脈粥樣硬化的文獻中，“西方”飲食通常被描述為含有 20-23% 乳脂/乳脂、0.2% 總膽固醇和 34% 蔗糖的純化嚙齒動物飲食。 TD.88137 是“西式”飲食的一個例子，最初旨在表征和增強新生成的 Apoe 缺陷小鼠模型中的動脈粥樣硬化發展。聯系我們了解更多關于“西式”飲食、修改或可能的控制飲食的信息。

Examples:

TD.88137 ??? Adjusted calories diet (42% from fat, 0.2% total cholesterol)
TD.10885 ? 45% fat Kcal diet (0.2% total cholesterol)

Research use:

Accelerated hypercholesterolemia and plaque formation in genetically-modified models, such as Apoe and Ldlr deficient mice.

Used for diet-induced obesity in a variety of rodent models.

Key dietary features:

High Fat Diet (20-23% by weight; 40 – 45% kcal from fat)
Saturated fatty acids (SFA >60% of total fatty acids)
Milkfat/butterfat
Sucrose (34% by weight)
Cholesterol (0.2% total)

研究用途：

在基因改造模型（例如 Apoe 和 Ldlr 缺陷小鼠）中加速高膽固醇血癥和斑塊形成。

用于各種嚙齒動物模型中的飲食引起的肥胖。

主要飲食特點：

高脂肪飲食（20-23% 重量；40-45% 來自脂肪的大卡）
飽和脂肪酸（SFA > 60% 的總脂肪酸）
乳脂/乳脂
蔗糖（按重量計 34%）
膽固醇（總量 0.2%）

References:? 參開文獻

Febbraio, M., et al., Targeted disruption of the class B scavenger receptor CD36 protects against atherosclerotic lesion development in mice. J Clin Invest, 2000. 105(8): p. 1049-56.
Huszar, D., et al., Increased LDL cholesterol and atherosclerosis in LDL receptor-deficient mice with attenuated expression of scavenger receptor B1. Arterioscler Thromb Vasc Biol, 2000. 20(4): p. 1068-73.
Nakashima, Y., et al., ApoE-deficient mice develop lesions of all phases of atherosclerosis throughout the arterial tree. Arterioscler Thromb, 1994. 14(1): p. 133-40.
Nakashima, Y., et al., Upregulation of VCAM-1 and ICAM-1 at atherosclerosis-prone sites on the endothelium in the ApoE-deficient mouse. Arterioscler Thromb Vasc Biol, 1998. 18(5): p. 842-51.
Plump, A.S., et al., Severe hypercholesterolemia and atherosclerosis in apolipoprotein E-deficient mice created by homologous recombination in ES cells. Cell, 1992. 71(2): p. 343-53.
Towler, D.A., et al., Diet-induced diabetes activates an osteogenic gene regulatory program in the aortas of low density lipoprotein receptor-deficient mice. J Biol Chem, 1998. 273(46): p. 30427-34.
Tsuchiya, K., et al., FoxOs integrate pleiotropic actions of insulin in vascular endothelium to protect mice from atherosclerosis. Cell Metab, 2012. 15(3): p. 372-81.

“Western” purified atherogenic diet with added cholesterol and cholate source*

Wild type mice and rats generally are resistant to atherosclerosis, requiring more extreme dietary manipulation to modify lipoprotein profiles and develop mild atherosclerosis (foam cells, fatty streaks). Modern formulations are made completely of purified ingredients because this more refined approach has been reported to decrease the incidence of gallstones and liver damage associated with less refined and more traditional dietary approaches. To induce mild atherosclerosis in wild type animals, the “Western” purified diet can be modified to increase cholesterol (1-1.25%) and add a bile salt such as sodium cholate or cholic acid. Contact us for more information, modifications, or possible control diets.

添加膽固醇和膽酸鹽來源的“西方”純化致動脈粥樣硬化飲食*
野生型小鼠和大鼠通常對動脈粥樣硬化有抵抗力，需要更極端的飲食操作來改變脂蛋白譜并發展為輕度動脈粥樣硬化（泡沫細胞、脂肪條紋）。現代配方完全由純化成分制成，因為據報道這種更精致的方法可以降低與不太精致和更傳統的飲食方法相關的膽結石和肝損傷的發生率。為了在野生型動物中誘發輕度動脈粥樣硬化，可以修改“西方”純化飲食以增加膽固醇（1-1.25%）并添加膽汁鹽，例如膽酸鈉或膽酸。聯系我們了解更多信息、修改或可能的控制飲食。

Examples of purified high fat diets with added cholesterol and cholate source*:

- TD.02028 21% milkfat (1.25% cholesterol, 0.5% cholic acid)

- TD.09237 ?? 15% milkfat diet (1% cholesterol, 0.5% sodium cholate)

Examples of purified high fat diets with added cholesterol (without cholate source):

TD.96121 ??? 21% milkfat (1.25% cholesterol)

Research Use:

Induce hypercholesterolemia and mild atherosclerosis (foam cells, fatty streaks) primarily in wild type mice and rats.

Will not promote obesity.

研究用途：

主要在野生型小鼠和大鼠中誘發高膽固醇血癥和輕度動脈粥樣硬化（泡沫細胞、脂肪條紋）。

不會促進肥胖。

Key dietary features:

High fat diet (15-20% by weight; 34 – 45% kcal from fat)
Saturated fatty acids (SFA >55% of total fatty acids)
Milkfat/butterfat, cocoa butter
Sucrose (30-50% by weight)
Cholesterol (1 – 1.25%)
Cholate source (0.5%)*

主要飲食特點：

高脂肪飲食（15-20% 重量；34-45% 來自脂肪的大卡）
飽和脂肪酸（SFA > 55% 的總脂肪酸）
乳脂/乳脂、可可脂
蔗糖（按重量計 30-50%）
膽固醇 (1 – 1.25%)
膽酸鹽來源 (0.5%)*

References: 參考文獻

Bernal, C., et al., Lipid biomarkers and metabolic effects of lycopene from tomato juice on liver of rats with induced hepatic steatosis. J Nutr Biochem, 2013. 24(11): p. 1870-81.
Gao, Q., et al., Atherogenic diets exacerbate colitis in mice deficient in glutathione peroxidase. Inflamm Bowel Dis, 2010. 16(12): p. 2043-54.
Lichtman, A.H., et al., Hyperlipidemia and atherosclerotic lesion development in LDL receptor-deficient mice fed defined semipurified diets with and without cholate. Arterioscler Thromb Vasc Biol, 1999. 19(8): p. 1938-44.
Marcondes, M.C., et al., Effects of chronic mental stress and atherogenic diet on the immune inflammatory environment in mouse aorta. Brain Behav Immun, 2011. 25(8): p. 1649-57.
Nishina, P.M., et al., Effects of dietary fats from animal and plant sources on diet-induced fatty streak lesions in C57BL/6J mice. J Lipid Res, 1993. 34(8): p. 1413-22.
Nishina, P.M., et al., Atherosclerosis and plasma and liver lipids in nine inbred strains of mice. Lipids, 1993. 28(7): p. 599-605.
Yue, P., et al., Enhanced hepatic apoA-I secretion and peripheral efflux of cholesterol and phospholipid in CD36 null mice. PLoS One, 2010. 5(3): p. e9906.
Nishina, P.M., J. Verstuyft, and B. Paigen, Synthetic low and high fat diets for the study of atherosclerosis in the mouse. J Lipid Res, 1990. 31(5): p. 859-69.

*Sodium cholate or cholic acid aid cholesterol and fat absorption and reduce cholesterol disposal via bile acid synthesis. However, if including a cholate source is not desired for your research, diets without cholate are available. See TD.96121 for a purified diet and TD.94059 for a hybrid diet. Contact us for additional options.

*膽酸鈉或膽酸有助于膽固醇和脂肪的吸收，并通過膽汁酸合成減少膽固醇的排放。但是，如果您的研究不需要包含膽酸鹽來源，則可以使用不含膽酸鹽的飲食。純化飲食參見 TD.96121，混合飲食參見 TD.94059。聯系我們獲取更多選項。

Hybrid high fat diets with added cholesterol and cholate source*

Beverly Paigen and colleagues first characterized atherosclerosis development in C57BL/6 mice by feeding a hybrid atherogenic diet. The hybrid diet was created by mixing a natural ingredient mouse diet in a 3:1 ratio with a concentrated purified diet (containing 5% cholesterol and 2% sodium cholate; referred to as Thomas-Hartroft diet). The resulting mixture recreated in TD.88051 /TD.90221 (same formula) contains ~15.8% fat, 1.25% cholesterol, and 0.5% sodium cholate. This group later compared the hybrid atherogenic diet approach to the more modern “western” purified atherogenic diet with added cholesterol and cholate and found that the hybrid atherogenic diet induced more gallstones and liver damage. Hybrid diets contain a variety of unrefined ingredients that may modify lipid metabolism and atherogenesis and do not allow for precise control of ingredients and nutrients for the study of chronic diseases. Although more refined diets have been developed, hybrid atherogenic diets are still popular for inducing mild atherosclerosis and gallstones in wild type mice and rats. Contact us for more information, modifications, or possible control diets.

添加膽固醇和膽酸鹽來源的混合高脂肪飲食*
Beverly Paigen 及其同事首先通過喂養混合致動脈粥樣硬化飲食來表征 C57BL/6 小鼠的動脈粥樣硬化發展?；旌巷嬍呈峭ㄟ^將天然成分的小鼠飲食以 3:1 的比例與濃縮的純化飲食（含有 5% 的膽固醇和 2% 的膽酸鈉；稱為 Thomas-Hartroft 飲食）混合而成的。在 TD.88051 /TD.90221（相同配方）中重新生成的混合物含有約 15.8% 的脂肪、1.25% 的膽固醇和 0.5% 的膽酸鈉。該小組后來將混合致動脈粥樣硬化飲食方法與更現代的“西方”純化致動脈粥樣硬化飲食（添加膽固醇和膽酸鹽）進行了比較，發現混合致動脈粥樣硬化飲食會導致更多的膽結石和肝損傷?；旌巷嬍澈卸喾N未精制的成分，這些成分可能會改變脂質代謝和動脈粥樣硬化形成，并且不允許對慢性疾病研究中的成分和營養素進行精確控制。盡管已經開發出更精細的飲食，但混合致動脈粥樣硬化飲食仍然很受歡迎，可在野生型小鼠和大鼠中誘發輕度動脈粥樣硬化和膽結石。聯系我們了解更多信息、修改或可能的控制飲食。

Examples of hybrid high-fat diets with added cholesterol and cholate source*:

TD.88051 and TD.90221 (same formula) are Teklad product codes for hybrid atherogenic diets

Example of hybrid high-fat diet with added cholesterol (without cholate source):

TD.94059

Research Use:

Induce hypercholesterolemia and mild atherosclerosis (foam cells, fatty streaks) primarily in wild type mice and rats.

Will not promote obesity.

Also used for lithogenic (gallstone) rodent studies.

Key dietary features:

- 75% rodent breeder diet; 25% purified ingredients

- High fat (~15% by weight; 37% kcal from fat)

- Saturated fatty acids (SFA >45% of total fatty acids)

- Cholesterol (1.25%)

- Cholate source (0.5%)*

主要飲食特點：

75% 嚙齒動物飼養者飲食； 25% 純化成分
高脂肪（約 15% 重量；37% 來自脂肪大卡）
飽和脂肪酸（SFA > 總脂肪酸的 45%）
膽固醇 (1.25%)
膽酸鹽來源 (0.5%)*

References: 參考文獻

Nishina, P.M., J. Verstuyft, and B. Paigen, Synthetic low and high fat diets for the study of atherosclerosis in the mouse. J Lipid Res, 1990. 31(5): p. 859-69.
Clee, S.M., et al., Plasma and vessel wall lipoprotein lipase have different roles in atherosclerosis. J Lipid Res, 2000. 41(4): p. 521-31.
George, J., et al., Enhanced fatty streak formation in C57BL/6J mice by immunization with heat shock protein-65. Arterioscler Thromb Vasc Biol, 1999. 19(3): p. 505-10.
Miyake, J.H., et al., Transgenic expression of cholesterol-7-alpha-hydroxylase prevents atherosclerosis in C57BL/6J mice. Arterioscler Thromb Vasc Biol, 2002. 22(1): p. 121-6.
Paigen, B., et al., Quantitative assessment of atherosclerotic lesions in mice. Atherosclerosis, 1987. 68(3): p. 231-40.
Schreyer, S.A., D.L. Wilson, and R.C. LeBoeuf, C57BL/6 mice fed high fat diets as models for diabetes-accelerated atherosclerosis. Atherosclerosis, 1998. 136(1): p. 17-24.
Vergnes, L., et al., Cholesterol and cholate components of an atherogenic diet induce distinct stages of hepatic inflammatory gene expression. J Biol Chem, 2003. 278(44): p. 42774-84.

Standard diets with added cholesterol

Standard, natural ingredient diets with cholesterol added are fed to induce hypercholesterolemia. Various levels of cholesterol, fat, and/or bile salts can be added to one of the numerous standard rodent diets stocked by?Envigo Teklad. For many applications, adding these components to Envigo’s minimal-to-moderate phytoestrogen global rodent diets is recommended. Our minimal phytoestrogen global rodent diets are soybean meal free, limiting the effect of phytoestrogens on your research outcomes. Soybean meal, a common dietary source of phytoestrogens, has been shown to decrease aortic fatty streak development and modify plasma cholesterol, which may reduce the risk of developing atherosclerosis. Limiting dietary soybean meal may reduce confounding variables within your dietary-induced atherosclerosis model. Contact a nutritionist to discuss additional diet options.

添加膽固醇的標準飲食
喂食添加膽固醇的標準天然成分飲食以誘發高膽固醇血癥。 Envigo Teklad 庫存的眾多標準嚙齒動物飲食之一中可以添加不同水平的膽固醇、脂肪和/或膽汁鹽。對于許多應用，建議將這些成分添加到 Envigo 的最低至中度植物雌激素全球嚙齒動物飲食中。我們的最低植物雌激素全球嚙齒動物飲食不含豆粕，限制了植物雌激素對您的研究成果的影響。豆粕是植物雌激素的常見膳食來源，已被證明可以減少主動脈脂肪條紋的形成并改變血漿膽固醇，這可能會降低患動脈粥樣硬化的風險。限制膳食豆粕可能會減少膳食誘導的動脈粥樣硬化模型中的混雜變量。聯系營養師討論其他飲食選擇。

Examples of minimal and moderate phytoestrogen rodent diets with added cholesterol:

- TD.120097 ? 1% cholesterol diet (2020 – minimal phytoestrogens)

- TD.07841 ??? 2% cholesterol diet (2016 – minimal phytoestrogens)

- TD.01383 ??? 2% cholesterol (2018 – Moderate phytoestrogens)

Research use:

Induce hypercholesterolemia in genetically-modified and wild type models without promoting obesity.

Key dietary features:

- Standard, grain-based rodent diet

- Minimal/moderate phytoestrogen diets recommended

- Cholesterol (1 – 4%)

References: 參考文獻

Belch, J.J., et al., Longitudinal assessment of endothelial function in the microvasculature of mice in-vivo. Microvasc Res, 2013. 85: p. 86-92.
Hartvigsen, K., et al., A diet-induced hypercholesterolemic murine model to study atherogenesis without obesity and metabolic syndrome. Arterioscler Thromb Vasc Biol, 2007. 27(4): p. 878-85.

Diets for additional animal models of atherosclerosis

Rabbits, hamsters, and swine are common models of atherosclerosis. Contact a nutritionist for information and formula examples. See?rabbit, swine?and other species?for information and formula examples.

For additional phytoestrogen information, see our list of?phytoestrogen references

其他動脈粥樣硬化動物模型的飲食
兔子、倉鼠和豬是動脈粥樣硬化的常見模型。聯系營養師獲取信息和配方示例。有關信息和配方示例，請參閱兔子、豬和其他物種。

有關其他植物雌激素信息，請參閱我們的植物雌激素參考列表

Live chatLive chat ?

Harlan 定制飲食誘發肥胖Custom diet induced obesity

發表于2022年1月13日由上海金畔生物科技有限公司

Harlan 定制飲食誘發肥胖Custom diet induced obesity

Harlan Teklad動物飼料
歡迎新老客戶訪問Harlan官網或者咨詢上海金畔生物獲取更多詳細資料。

Diet induced obesity

Purified high fat diets used to induce obesity and obesity-related complications such as diabetes and metabolic syndrome typically have 40-60% of energy derived from fat. The diet tables below summarize relevant diet features for several Teklad custom research diets commonly used in rodent models.

Teklad also creates high-fat diets for other species, including pigs, primates, and dogs.?Contact us?to discuss the use of these diets or one that better meets your needs.

用于誘發肥胖和肥胖相關并發癥（如糖尿病和代謝綜合征）的純化高脂肪飲食通常有 40-60% 的能量來自脂肪。下面的飲食表總結了嚙齒動物模型中常用的幾種 Teklad 定制研究飲食的相關飲食特征。

Teklad 還為其他物種創造高脂肪飲食，包括豬、靈長類動物和狗。聯系我們討論如何使用這些飲食或一種更能滿足您需求的飲食。

order today

18301939375

上海金畔生物科技有限公司

Commonly-used diet-induced obesity (DIO) Teklad rodent diets with 55-60% of calories from fat

Diet features	TD.06414 stocked	TD.93075 dough	TD.07011 pellet
Kcal/g	5.1	4.8	4.8
Fat, % Kcal	60	55	55
Fat Sources,% by weight	31% lard 3% soybean oil	27.4% vegetable shortening 1.6% corn oil	27.4% vegetable shortening 1.6% corn oil
Calculated fatty acid profile, % total fat	36% saturated 41% monounsaturated 23% polyunsaturated	24% saturated 61% monounsaturated (cis + trans) 15% polyunsaturated (cis + trans)	24% saturated 61% monounsaturated (cis + trans) 15% polyunsaturated (cis + trans)
Sucrose, % by weight	12.1	9.6	9.6
Notes	60F10S poster data Compare to D12492	Trans fat	Trans fat
Example modifications	TD.08500 coconut oil TD.09766 milk fat
Ingredient matched, low fat control diets*	TD.06416 (35% sucrose) TD.08806 (11% sucrose)	TD.93074 (21% sucrose) TD.120651 (7% sucrose)	TD.93074 (21% sucrose) TD.120651 (7% sucrose)
References	Mouse Rat	Mouse Rat	Mouse Rat

*Control diets can be designed in several ways, depending on what features the researcher wants to modify relative to the high-fat diet. These are just a few examples.

對照飲食可以通過多種方式設計，具體取決于研究人員相對于高脂肪飲食想要修改的特征。這些只是幾個例子。

Commonly-used diet-induced obesity (DIO) Teklad rodent diets with 40-45% of calories from fat

常用的飲食誘導肥胖 (DIO) Teklad 嚙齒動物飲食，其中 40-45% 的卡路里來自脂肪

Diet features	TD.06415	TD.08811	TD.88137 stocked	TD.95217
Kcal/g	4.6	4.7	4.5	4.3
Fat, % Kcal	45	45	42	40
Fat sources, % by weight	19.5% lard 3% soybean oil	21% milk fat 2% soybean oil	21% milk fat	10.6% vegetable shortening 4% milk fat 4% soybean oil
Fatty acid profile,% total fat	35% saturated 40% monounsaturated 25% polyunsaturated	61% saturated 30% monounsaturated 9% polyunsaturated	66% saturated 30% monounsaturated 4% polyunsaturated	31% saturated 47% monounsaturated (cis + trans) 22% polyunsaturated (cis + trans)
Sucrose,% by weight	22.8	36.8	34.5	15.8
Notes	Compare to D12451	45F30S poster data	“Western Diet”Cholesterol added	Trans fat
Example modifications	TD.110716 milk fat TD.10670 no dye	TD.130784 lard TD.120438 no dye	TD.07201 lard TD.00573 h-coconut oil, no cholesterol TD.09682 blue dye	TD.07734 green dye
Ingredient matched, low fat control diets*	TD.06416 (35% sucrose) TD.110675 (18% sucrose)	TD.120455 (6% sucrose, resistant starch) TD.120724 (14% sucrose)	TD.05230 (34% sucrose) TD.08485 (12% sucrose)	TD.06101 (6% sucrose)
References	Mouse Rat	Mouse Rat	Mouse Rat	Mouse Rat

Diets for diet-induced obesity (DIO)

Diets with 55-60% of calories from fat like TD.06414 and TD.93075 are commonly used for inducing obesity in rodents. While considered extreme compared to typical human fat consumption, these diets are effective in initiating rapid weight gain in most rodents. With higher fat content there is less room for carbohydrate, thus the carbohydrate (particularly sucrose) amount is relatively low compared to other obesity inducing diets. If you are interested in high fat and high carbohydrate, look at diets with 40-45% of calories from fat (often referred to as western diets).

As the fat level increases, pellet quality (durability) is often compromised. Some higher fat formulas are available only in non-pelleted form or require specific carbohydrate, maltodextrin, for pelleting. Depending on the fat and carbohydrate sources used, the non-pelleted form could be dense and crumbly, dough-like, or paste-like. Though a little more challenging to work with, non-pelleted diet is still used by many researchers for diet-induced obesity models as these researchers suspect the softer form may enhance obesity development.

Diets with 40-45% of calories from fat, like TD.95217 , TD.88137 , TD.06415 , and TD.08811 , represent another popular diet pattern for diet-induced obesity work. These diets have double or triple the amount of sucrose found in higher fat diets. High levels of simple carbohydrate like sucrose and fructose may help to promote hypertriglyceridemia, insulin resistance, and fatty liver. Diets with a pattern of high sucrose and high saturated or trans fat are often referred to as “Western Diets” in obesity and cardiovascular fields. Some “Western Diets” have further modifications to the fatty acid profile or even specific vitamin and minerals adjustments to be even more closely matched to a Western Diet pattern. For specific fatty acid modifications, see examples on our fat/lipid adjusted diets page.

Diets for diet-induced diabetes

Many of the same diets used for inducing obesity in rodents can be used to enhance diabetes related phenotypes like insulin resistance and glucose intolerance. However, fasting hyperglycemia characteristic of diabetes (glucose > 200 mg/dL) is uncommon with a diet only approach. Pre-feeding a high fat diet to induce a certain level of obesity and insulin resistance and then giving low-dose streptozotocin (STZ) may be an effective approach if overt hyperglycemia is desired.

Ingredient matched, low-fat DIO control diets

There are many options with different levels and types of fat in addition to different types of carbohydrate ranging from sucrose (highly refined, simple digestion) to corn starch (refined, but more complex) to resistant starch (refined, but not fully digestible). A very basic purified control diet would be AIN-93M (TD.94048 ) or AIN-93G (TD.94045 ). AIN-93 diets have a moderate amount of sucrose at ~10%, and fat is from soybean oil with a healthy fatty acid profile. Additional examples of controls for specific DIO diets can be found in the above tables. Learn more about AIN diet formulas.

Many researchers choose to compare their high fat fed animals to animals fed a natural ingredient, grain-based diet (also referred to as standard diets or chow). These diets differ in the source and level of nutrients as well as in the presence of non-nutritive factors (such as phytates or phytoestrogens). Depending on what your main comparisons are, it may be suitable to have a grain-based diet as your control/reference group. However, making such comparisons limits inferences to dietary patterns versus a specific dietary component.

飲食引起的肥胖癥 (DIO)

TD.06414 和 TD.93075 等 55-60% 熱量來自脂肪的飲食通常用于誘導嚙齒動物肥胖。雖然與典型的人類脂肪消耗相比，這些飲食被認為是極端的，但這些飲食在大多數嚙齒動物開始快速體重增加方面是有效的。由于脂肪含量較高，碳水化合物的空間較小，因此與其他導致肥胖的飲食相比，碳水化合物（尤其是蔗糖）的量相對較低。如果您對高脂肪和高碳水化合物感興趣，請查看 40-45% 卡路里來自脂肪的飲食（通常稱為西方飲食）。

隨著脂肪含量的增加，顆粒質量（耐用性）通常會受到影響。一些高脂肪配方僅以非顆粒形式提供，或者需要特定的碳水化合物麥芽糖糊精來制成顆粒。根據所使用的脂肪和碳水化合物來源，非顆粒狀可以是致密易碎的、面團狀或糊狀的。盡管使用起來更具挑戰性，但許多研究人員仍將非顆粒狀飲食用于飲食誘導的肥胖模型，因為這些研究人員懷疑較軟的形式可能會促進肥胖的發展。

40-45% 熱量來自脂肪的飲食，如 TD.95217、TD.88137、TD.06415 和 TD.08811，代表了另一種流行的飲食誘導肥胖工作的飲食模式。這些飲食中的蔗糖含量是高脂肪飲食中的兩倍或三倍。高水平的簡單碳水化合物如蔗糖和果糖可能有助于促進高甘油三酯血癥、胰島素抵抗和脂肪肝。具有高蔗糖和高飽和或反式脂肪模式的飲食在肥胖和心血管領域通常被稱為“西方飲食”。一些“西方飲食”對脂肪酸成分進行了進一步的修改，甚至對特定的維生素和礦物質進行了調整，以更加接近西方飲食模式。對于特定的脂肪酸修飾，請參閱我們的脂肪/脂質調整飲食頁面上的示例。

飲食誘發糖尿病的飲食
許多用于誘導嚙齒動物肥胖的相同飲食可用于增強糖尿病相關表型，如胰島素抵抗和葡萄糖耐受不良。然而，僅通過飲食的方法很少出現糖尿病的空腹高血糖特征（葡萄糖 > 200 mg/dL）。如果需要明顯的高血糖，預先喂食高脂肪飲食以誘導一定程度的肥胖和胰島素抵抗，然后給予低劑量的鏈脲佐菌素 (STZ) 可能是一種有效的方法。

成分匹配的低脂 DIO 控制飲食
除了不同類型的碳水化合物，從蔗糖（高度精制，簡單消化）到玉米淀粉（精制，但更復雜）到抗性淀粉（精制，但不完全消化），還有許多脂肪含量和類型不同的選擇。一個非?；镜募兓瘜φ诊嬍呈?AIN-93M (TD.94048) 或 AIN-93G (TD.94045)。 AIN-93 日糧含有適量的蔗糖，約為 10%，脂肪來自具有健康脂肪酸成分的大豆油。特定 DIO 飲食控制的其他示例可在上表中找到。了解更多關于 AIN 飲食配方的信息。

許多研究人員選擇將他們喂食高脂肪的動物與喂食天然成分、谷物飲食（也稱為標準飲食或食物）的動物進行比較。這些飲食的營養來源和水平以及非營養因素（如植酸鹽或植物雌激素）的存在不同。根據您的主要比較，以谷物為主的飲食作為您的對照組/參考組可能是合適的。然而，進行這樣的比較限制了對飲食模式與特定飲食成分的推斷。

Live chatLive chat ?

Harlan 碳水化合物調整飼料Carbohydrate adjusted

發表于2022年1月13日由上海金畔生物科技有限公司

Harlan 碳水化合物調整飼料Carbohydrate adjusted

Harlan Teklad動物飼料
歡迎新老客戶訪問Harlan官網或者咨詢上海金畔生物獲取更多詳細資料。

Carbohydrate adjusted

A few carbohydrate adjusted formulas are shown below. Please contact us for additional formulas of this nature or for more information about altering the carbohydrate profile of a diet.

一些碳水化合物調整公式如下所示。請聯系我們以獲取此類性質的其他配方或有關改變飲食中碳水化合物成分的更多信息。

Formula examples:

TD.89247 ????? 60% Fructose diet
TD.86489 ????? Diet with adjusted sucrose/cornstarch
TD.96348 ????? Diet (20% lactose, 2% Ca, 1.25% P)
TD.98090 ????? 70% Carbohydrate diet
TD.96355 ????? Ketogenic diet (almost no carbohydrate)

公式示例：

TD.89247 60% 果糖飲食
TD.86489 調整蔗糖/玉米淀粉的飲食
TD.96348 飲食（20% 乳糖、2% 鈣、1.25% 磷）
TD.98090 70% 碳水化合物飲食
TD.96355 生酮飲食（幾乎不含碳水化合物）

order today

18301939375

上海金畔生物科技有限公司

Carbohydrates often make up the majority (by weight and % kcal) of custom research diets, with the exception of higher fat diets. Commonly used carbohydrate sources include sucrose, cornstarch and maltodextrin. Other sources include fructose, dextrose (glucose), dextrin, and lactose. Maltodextrin is enzymatically derived from cornstarch to have shorter glucose polymers and is helpful in pelleting high fat diets and in diets where cornstarch exceeds sucrose.

Sucrose is a part of most formulas, often making up 10% of the diet or more. This likely adds to the palatability of the diet. The carbohydrate profile of a diet can be manipulated, although some mixtures may not pellet. There are also diets with very little carbohydrate, thus containing high amounts of fat and/or protein.

Cellulose (fiber) is part of many formulas and while not required by rodents, is considered beneficial. It can also be used to make formulas with differing macronutrient profiles isocaloric.

碳水化合物通常占定制研究飲食的大部分（按重量和百分比 kcal），高脂肪飲食除外。常用的碳水化合物來源包括蔗糖、玉米淀粉和麥芽糖糊精。其他來源包括果糖、右旋糖（葡萄糖）、糊精和乳糖。麥芽糖糊精是從玉米淀粉酶促衍生的，具有較短的葡萄糖聚合物，有助于高脂肪飲食和玉米淀粉超過蔗糖的飲食的制粒。

蔗糖是大多數配方食品的一部分，通常占飲食的 10% 或更多。這可能會增加飲食的適口性。可以控制飲食中的碳水化合物成分，盡管某些混合物可能不會形成顆粒。還有一些碳水化合物很少的飲食，因此含有大量的脂肪和/或蛋白質。

纖維素（纖維）是許多配方的一部分，雖然嚙齒動物不需要，但被認為是有益的。它還可用于制作具有不同常量營養素特征的等熱量配方。

Live chatLive chat ?

圖片僅供參考,請以實物為準。

Harlan 氯化鈉調整（天然成分）飼料NaCl adjusted (natural ingredient)

發表于2022年1月13日由上海金畔生物科技有限公司

Harlan 氯化鈉調整（天然成分）飼料NaCl adjusted (natural ingredient)

Harlan Teklad動物飼料
歡迎新老客戶訪問Harlan官網或者咨詢上海金畔生物獲取更多詳細資料。

NaCl adjusted (natural ingredient)

The sodium content of natural ingredients such as corn, wheat, and soybean meal is low. Thus, these ingredients can be used to create a base diet that is sodium deficient. To this base diet, various amounts of sodium chloride salt (NaCl) can be added, and other ingredients adjusted slightly to maintain a relatively constant nutrient profile (with the exception of sodium and chloride).

Below are examples from a popular series of adjusted NaCl diets. Contact us for more information.

玉米、小麥、豆粕等天然成分的鈉含量較低。因此，這些成分可用于制造缺乏鈉的基礎飲食。在這種基礎飲食中，可以添加不同量的氯化鈉鹽 (NaCl)，并稍微調整其他成分以保持相對恒定的營養成分（鈉和氯化物除外）。

以下是一系列流行的調整 NaCl 飲食的示例。聯系我們獲取更多信息。

order today

18301939375

上海金畔生物科技有限公司

Formula examples
Added NaCl (%)	Approx. Na (%)	no dye	red* dye	orange* dye	blue* dye
Na deficient	0.01-0.02	TD.90228	TD.08290
Possible control diets: 0.05% Na (0.1% NaCl) meets est. minimum Na requirement. Typical rodent diets contain 0.2%-0.4% Na (~0.5-1% NaCl).
0.1	0.05	TD.94268		TD.07334
0.49	0.2	TD.96208		TD.110765
1	0.4	TD.90229
Diets for studies that look at effects of excess sodium consumption in rodents.
2	0.8	TD.95078		TD.130345
4	1.6	TD.92034	TD.110078		TD.03095
8	3.2	TD.92012	TD.03142

* When added to natural ingredient diets, these water soluble food dyes offer a slight color tint. More intense color can be achieved at higher inclusion rates.

Ask a nutritionist about additional salt concentrations or color options.

These diets are grain-based, with no animal derived ingredients, and have a background sodium content of approx. 0.01-0.02% and a background chloride content of approx. 0.06-0.07%. The selected nutrient content of the diets are as follows: approximately 19% protein, 5% fat, 3% crude fiber, 0.86% Ca, 0.64% P, 0.72% K, and 0.15% Mg.

NaCl adjusted diets are often fed to dahl salt-sensitive/resistant (rapp) inbred rats. These rats are maintained on Teklad traditional diet 7034 (0.12% NaCl) within maximum security production facilities.

* 當添加到天然成分飲食中時，這些水溶性食用染料會提供輕微的色調。在更高的包含率下可以獲得更強烈的顏色。

向營養師詢問額外的鹽濃度或顏色選擇。

這些飲食以谷物為基礎，不含動物源性成分，背景鈉含量約為。 0.01-0.02%，背景氯化物含量約為。 0.06-0.07%。選擇的日糧營養成分如下：大約 19% 的蛋白質、5% 的脂肪、3% 的粗纖維、0.86% 的鈣、0.64% 的磷、0.72% 的鉀和 0.15% 的鎂。

NaCl 調整的飲食通常喂給對 dahl 鹽敏感/抗性 (rapp) 近交系大鼠。這些大鼠在最大安全生產設施內以 Teklad 傳統飲食 7034 (0.12% NaCl) 維持。

Live chatLive chat ?

Harlan NAFLD and NASH 飼料

發表于2022年1月13日由上海金畔生物科技有限公司

Harlan NAFLD and NASH 飼料

Harlan Teklad動物飼料
歡迎新老客戶訪問Harlan官網或者咨詢上海金畔生物獲取更多詳細資料。

NAFLD and NASH

Dietary methods to induce NAFLD/NASH in rodents can be split into two common categories:

diets fed for longer periods of time to induce obesity, metabolic syndrome, and mild NASH or
diets fed for short periods of time to induce hepatic features of severe NASH without inducing obesity or insulin resistance

This page provides further information on dietary methods to induce NAFLD/NASH. We’ve also prepared a downloadable NASH/NAFLD mini paper.

The tables below highlight diet options from both of the above categories. For more complete descriptions of NAFLD/NASH models see the drop down menus that follow the tables.

在嚙齒動物中誘發 NAFLD/NASH 的飲食方法可分為兩個常見類別：

喂食較長時間的飲食會誘發肥胖、代謝綜合征和輕度 NASH 或
短時間喂食以誘發嚴重 NASH 的肝臟特征而不誘發肥胖或胰島素抵抗的飲食
本頁提供了有關誘發 NAFLD/NASH 的飲食方法的更多信息。我們還準備了可下載的 NASH/NAFLD 迷你論文。

下表突出顯示了上述兩個類別的飲食選擇。有關 NAFLD/NASH 模型的更完整描述，請參見表格后面的下拉菜單。

order today

18301939375

上海金畔生物科技有限公司

Diet options for inducing obesity, metabolic syndrome and mild NAFLD/NASH

Diet features	Western/Fast Food	ALIOS	FPC diet
Product Code	TD.88137	TD.06303	TD.160785 PWD dough
Fat, % Kcal	42	45	52
Fat Sources, % by weight	21% milk fat	22% hydrogenated vegetable oil 1% soybean oil	19% hydrogenated vegetable oil 6% milk fat 4% palmitic acid
Fatty acid profile, % total fat	66% saturated 30% monounsaturated 4% polyunsaturated	23% saturated 31% monounsaturated (cis) 12% polyunsaturated (cis) 34% trans	43% saturated 47% monounsaturated (cis + trans) 10% polyunsaturated (cis + trans)
Sugars, % by weight	34.5% sucrose	22.4% sucrose	34.5% sucrose
Cholesterol, % by weight	0.2	0	1.25
Modifications	TD.96121 1.25% cholesterol TD.120528 Increased sucrose, 1.25% cholesterol	TD.120330 0.2% cholesterol TD.130885 0.2% cholesterol, 27% sucrose	TD.140154 adds customer supplied palmitic acid

For high fat diet options to induce uncomplicated NAFLD see our Diet Induced Obesity page.

有關誘發簡單 NAFLD 的高脂肪飲食選擇，請參閱我們的飲食誘發肥胖頁面。

Diet options for inducing more severe hepatic NAFLD/NASH without obesity or metabolic syndrome

Diet features	High Fat, Cholesterol & Cholate	Methionine/choline deficient (MCD)
Product Code	TD.02028	TD.90262
Fat, % Kcal	42	22
Fat Sources, % by weight	21% milk fat	10% corn oil
Fatty acid profile, % total fat	66% saturated 30% monounsaturated 4% polyunsaturated	14% saturated 28% monounsaturated 58% polyunsaturated
Sugars, % by weight	33.3% sucrose	46% sucrose
Cholesterol, % by weight	1.25	0
Cholate Source, % by weight	0.5	0
Related diets	TD.09237 15% milk fat, 1% cholesterol TD.88051 Hybrid version	TD.94149 MCD control diet

Diets inducing obesity, metabolic syndrome and mild NAFLD/NASH

Western or fast food style diets fed to induce NASH with metabolic syndrome contain 40 – 45% kcal from milkfat (a fat source high in palmitate) with added cholesterol (0.15 – 2%) and are high in sucrose (>30%). Dietary palmitate and cholesterol have both previously been associated with the progression from simple steatosis to NASH.

導致肥胖、代謝綜合征和輕度 NAFLD/NASH 的飲食
用于誘發患有代謝綜合征的 NASH 的西式或快餐式飲食含有 40 – 45% 來自乳脂（棕櫚酸酯含量高的脂肪來源）的 kcal，并添加了膽固醇（0.15 – 2%），并且蔗糖含量高（>30%）。膳食棕櫚酸酯和膽固醇以前都與從簡單脂肪變性到 NASH 的進展有關。

Examples:
- TD.88137 Adjusted Calories Diet (42% from fat)
- TD.96121 21% MF, 1.25% Chol. Diet
- TD.120528 42% Kcal/Fat Diet (Incr. Sucrose, 1.25% Chol.)
  
  Western and Fast Food diets with milkfat and cholesterol
Research use:

These diets can induce obesity, metabolic syndrome, and simple steatosis within nine weeks of feeding. Increased hepatic inflammation has been observed after 12 weeks of feeding. NASH typically requires longer feeding with fibrosis developing within nine months and late stage fibrosis including hepatic ballooning occurring after 14 – 20 months of feeding. Increasing dietary sucrose (~41%) and cholesterol (~1.25%) accelerates the NASH phenotype with steatosis, inflammation and hepatocyte ballooning observed within 12 weeks. In addition to feeding a high fat diet, providing a glucose/fructose mixture in the drinking water may further promote NASH development.

研究用途：

這些飲食會在喂食后的九周內誘發肥胖、代謝綜合征和單純性脂肪變性。喂養 12 周后觀察到肝臟炎癥增加。 NASH 通常需要更長的喂養時間，在 9 個月內出現纖維化，在 14-20 個月的喂養后出現包括肝氣球樣變在內的晚期纖維化。增加膳食蔗糖 (~41%) 和膽固醇 (~1.25%) 會加速 NASH 表型，在 12 周內觀察到脂肪變性、炎癥和肝細胞膨脹。除了喂食高脂肪飲食外，在飲用水中提供葡萄糖/果糖混合物可能會進一步促進 NASH 的發展。

Select References:? 參考文獻

Charlton, M., et al., Fast food diet mouse: novel small animal model of NASH with ballooning, progressive fibrosis, and high physiological fidelity to the human condition. Am J Physiol Gastrointest Liver Physiol, 2011. 301(5): p. G825-34. www.ncbi.nlm.nih.gov/pubmed/21836057

Gores, G., Charlton M, Krishnan A, Viker K, Sanderson S, Cazanave S, McConico A, Masuoko H. Am J Physiol Gastrointest Liver Physiol, 2015. 308: p. G159. ajpgi.physiology.org/content/308/2/G159

Li, Z.Z., et al., Hepatic lipid partitioning and liver damage in nonalcoholic fatty liver disease: role of stearoyl-CoA desaturase. J Biol Chem, 2009. 284(9): p. 5637-44. www.ncbi.nlm.nih.gov/pubmed/19119140

Ioannou, G.N., et al., Hepatic cholesterol crystals and crown-like structures distinguish NASH from simple steatosis. J Lipid Res, 2009. 54(5): p. 1326-34. www.ncbi.nlm.nih.gov/pubmed/23417738

Alkhouri, N., et al., Adipocyte apoptosis, a link between obesity, insulin resistance, and hepatic steatosis. J Biol Chem, 2010. 285(5): p. 3428-38. www.ncbi.nlm.nih.gov/pubmed/19940134

Dixon, L.J., et al., Caspase-1 as a central regulator of high fat diet-induced non-alcoholic steatohepatitis. PLoS One, 2013. 8(2): p. e56100. www.ncbi.nlm.nih.gov/pubmed/23409132

DeLeve, L.D., et al., Prevention of hepatic fibrosis in a murine model of metabolic syndrome with nonalcoholic steatohepatitis. Am J Pathol, 2008. 173(4): p. 993-1001. www.ncbi.nlm.nih.gov/pubmed/18772330

VanSaun, M.N., et al., High fat diet induced hepatic steatosis establishes a permissive microenvironment for colorectal metastases and promotes primary dysplasia in a murine model. Am J Pathol, 2009. 175(1): p. 355-64. www.ncbi.nlm.nih.gov/pubmed/19541928

Asgharpour, A., et al., A diet-induced animal model of non-alcoholic fatty liver disease and hepatocellular cancer. J Hepatol, 2016. 65(3): p. 579-88. www.ncbi.nlm.nih.gov/pubmed/27261415

Tetri, L.H., et al., Severe NAFLD with hepatic necroinflammatory changes in mice fed trans fats and a high-fructose corn syrup equivalent. Am J Physiol Gastrointest Liver Physiol, 2008. 295(5): p. G987-95. www.ncbi.nlm.nih.gov/pubmed/18772365

Tsuchida, T., et al., A simple diet-and chemical-induced murine NASH model with rapid progression of steatohepatitis, fibrosis and liver cancer. Journal of hepatology, 2018. 69(2):385-395. www.ncbi.nlm.nih.gov/pubmed/29572095
The ALIOS model: western diet with trans-fat
The American Lifestyle-Induced Obesity Syndrome (ALIOS) model involves feeding the “American fast food” diet high in trans-fats and sugar. Dietary trans-fats from hydrogenated vegetable shortening (HVO) are associated with increased insulin resistance and hepatic inflammation in rodent NASH models. In addition to diet, a glucose/fructose solution is added to the drinking water and sedentary behavior promoted by removing the overhead cage feeders in this model.

ALIOS 模型：含有反式脂肪的西方飲食
美國生活方式誘發的肥胖綜合癥 (ALIOS) 模型涉及喂食富含反式脂肪和糖的“美國快餐”飲食。來自氫化植物起酥油 (HVO) 的膳食反式脂肪與嚙齒動物 NASH 模型中胰島素抵抗和肝臟炎癥的增加有關。除了飲食，葡萄糖/果糖溶液被添加到飲用水中，并且通過移除該模型中的架空籠式喂食器來促進久坐行為。

Examples:
- TD.06303 22% HVO Diet
- TD.120330 22% HVO + 0.2% Cholesterol Diet
- TD.130885 ALIOS with Added Sugar
Research use:

The ALIOS model develops obesity with insulin resistance, elevated ALT levels, and steatosis within 16 weeks. Increased inflammation and early development of fibrosis have been observed at 6 months. Severe steatosis with fibrosis and inflammation develops within 12 months of feeding with 50% of the mice reportedly developing hepatic neoplasms. Adding cholesterol (0.2%) to the American Fast Food diet may accelerate NASH phenotype development.

研究用途：

ALIOS 模型在 16 周內發展為具有胰島素抵抗、ALT 水平升高和脂肪變性的肥胖癥。在 6 個月時觀察到炎癥增加和纖維化的早期發展。喂食后 12 個月內會出現伴有纖維化和炎癥的嚴重脂肪變性，據報道有 50% 的小鼠會出現肝腫瘤。在美國快餐飲食中添加膽固醇（0.2%）可能會加速 NASH 表型的發展。

Select References: 參考文獻

Koppe, S.W., et al., Trans fat feeding results in higher serum alanine aminotransferase and increased insulin resistance compared with a standard murine high-fat diet. Am J Physiol Gastrointest Liver Physiol, 2009. 297(2): p. G378-84. www.ncbi.nlm.nih.gov/pubmed/19541924

Tetri, L.H., et al., Severe NAFLD with hepatic necroinflammatory changes in mice fed trans fats and a high-fructose corn syrup equivalent. Am J Physiol Gastrointest Liver Physiol, 2008. 295(5): p. G987-95. www.ncbi.nlm.nih.gov/pubmed/18772365

Mells, J.E., et al., Glp-1 analog, liraglutide, ameliorates hepatic steatosis and cardiac hypertrophy in C57BL/6J mice fed a Western diet. Am J Physiol Gastrointest Liver Physiol, 2012. 302(2): p. G225-35. www.ncbi.nlm.nih.gov/pubmed/22038829

Dowman, J.K, et al., Development of hepatocellular carcinoma in a murine model of nonalcoholic steatohepatitis induced by use of a high-fat/fructose diet and sedentary lifestyle. Am J Pathol, 2014. 184(5):1550-1561. www.ncbi.nlm.nih.gov/pubmed/24650559

Mells, J.E., et al., Saturated fat and cholesterol are critical to inducing murine metabolic syndrome with robust nonalcoholic steatohepatitis. J Nutr Biochem, 2014. 26(3): p. 285-92. www.ncbi.nlm.nih.gov/pubmed/25577467
The Fructose, Palmitate, Cholesterol and Trans-Fat (FPC) diet is a recent NASH diet that includes Western and ALIOS model diets to achieve both metabolic and hepatic NASH features within an accelerated time frame. Key features of the FPC diet include 1) a lower Met content than typical rodent diets by decreasing total protein without supplementing sulfur amino acids; 2) choline supplementation is lower than typical but is not considered deficient; 3) high in sucrose (~34% by weight); 4) 1.25% cholesterol; 5) 52% kcal from fat with fat sources including milkfat fat, palmitic acid and hydrogenated vegetable shortening to provide trans-fats. Like the ALIOS model, the FPC model also provides a glucose/fructose solution to the drinking water.

FPC飲食：果糖、棕櫚酸酯、膽固醇和反式脂肪飲食
果糖、棕櫚酸酯、膽固醇和反式脂肪 (FPC) 飲食是最近的 NASH 飲食，包括西方和 ALIOS 模型飲食，以在加速的時間范圍內實現代謝和肝臟 NASH 特征。 FPC 飲食的主要特點包括 1) 通過減少總蛋白質而不補充含硫氨基酸，Met 含量低于典型嚙齒動物飲食； 2) 膽堿補充量低于典型值但不被認為是不足的； 3) 蔗糖含量高（約 34% 重量）； 4) 1.25% 膽固醇； 5) 52% kcal 來自脂肪來源，包括乳脂脂肪、棕櫚酸和氫化植物起酥油，以提供反式脂肪。與 ALIOS 模型一樣，FPC 模型也為飲用水提供葡萄糖/果糖溶液。

Examples:
- TD.160785 52 kcal/Fat Diet (C16:0, HVO, AMF, Choline/Met)
  
  FPC diet: fructose, palmitate, cholesterol and trans-fat diet
Research use:

Male C57BL/6J mice fed the FPC diet and provided a glucose/fructose drinking solution developed insulin resistance and NAFLD with inflammation, hepatocyte death, and fibrosis within 16 weeks.

研究用途：

喂食 FPC 飲食并提供葡萄糖/果糖飲用溶液的雄性 C57BL/6J 小鼠在 16 周內出現胰島素抵抗和 NAFLD，并伴有炎癥、肝細胞死亡和纖維化。

Select References:參考文獻

Wang, X., et al., Hepatocyte TAZ/WWTR1 promotes inflammation and fibrosis in nonalcoholic steatohepatitis. Cell Metab, 2016. 24(6): p. 848-62. www.ncbi.nlm.nih.gov/pubmed/28068223

Zhu, C., et al., Hepatocyte Notch activation induces liver fibrosis in nonalcoholic steatohepatitis. Sci Transl Med, 2018. 10(468). www.ncbi.nlm.nih.gov/pubmed/30463916
Common diets to induce obesity (DIO) can be fed to induce uncomplicated NAFLD. These high fat diets typically contain 40–60% kcal from fat without supplemented cholesterol or cholate. Simple sugars such as sucrose or fructose can also be supplemented via diet or water to progress the fatty liver phenotype. Diets can be in pellet or powder/dough form depending on the formula. Some models require limited physical activity and in those cases diets can be fed inside the cage. For more information see our Diet Induced Obesity page.

高脂肪飲食
可以喂食誘發肥胖癥 (DIO) 的普通飲食以誘發非復雜性 NAFLD。這些高脂肪飲食通常含有 40–60% 來自脂肪的大卡，而不含補充膽固醇或膽酸鹽。蔗糖或果糖等單糖也可以通過飲食或水補充，以促進脂肪肝表型。飲食可以是顆?；蚍勰?面團形式，具體取決于配方。有些模型需要有限的體力活動，在這些情況下，可以在籠子內喂食。欲了解更多信息，請參閱我們的飲食誘發肥胖頁面。

Examples:
- - TD.08811 45%kcal Fat Diet (21% MF, 2% SBO)
  - TD.06414 Adjusted Calories Diet (60/Fat)
    
    High fat diets
Research use:

In susceptible rodent models, high fat diets are commonly used to induce NAFLD with obesity and insulin resistance common metabolic features associated with NASH in humans. However, the degree of NASH pathology (steatosis, inflammation, and fibrosis) is limited or mild and varies depending on the animal model, length of feeding, and dietary components.

Diets to induce severe hepatic NAFLD/NASH without obesity or metabolic

Atherogenic diets high in fat, cholesterol, and cholate
Originally formulated to induce mild atherosclerosis in wild-type rodents, high fat diets containing added cholesterol (1 – 1.25%) and cholate (0.5% as sodium cholate or cholic acid) have also been useful in inducing NASH. This diet option includes purified “Western” style diets with increased cholesterol and cholate and also hybrid diets. Hybrid diets were originally developed by Beverly Paigen and colleagues by mixing a natural ingredient mouse diet in a 3:1 ratio with a concentrated purified diet (containing 5% cholesterol and 2% sodium cholate) resulting in a diet containing ~15.8% fat, 1.25% cholesterol, and 0.5% sodium cholate. Although a less refined approach, the hybrid diet is associated with increased gallstone formation and liver damage as compared to similar purified diets.

飲食可誘發嚴重的肝臟 NAFLD/NASH，但沒有肥胖或代謝
高脂肪、膽固醇和膽酸鹽的致動脈粥樣硬化飲食
最初配制用于在野生型嚙齒動物中誘導輕度動脈粥樣硬化，含有添加膽固醇（1 – 1.25%）和膽酸鹽（0.5% 作為膽酸鈉或膽酸）的高脂肪飲食也可用于誘導 NASH。這種飲食選擇包括增加膽固醇和膽酸鹽的純化“西式”飲食以及混合飲食。混合飲食最初是由 Beverly Paigen 及其同事開發的，通過將天然成分的小鼠飲食以 3:1 的比例與濃縮的純化飲食（含有 5% 膽固醇和 2% 膽酸鈉）混合，從而得到含有約 15.8% 脂肪、1.25 % 膽固醇和 0.5% 膽酸鈉。雖然是一種不太精細的方法，但與類似的純化飲食相比，混合飲食與增加的膽結石形成和肝損傷有關。

Examples:
- - TD.02028 Atherogenic Rodent Diet
  - TD.88051 Cocoa Butter Diet and Purina Mouse Chow
  - TD.09237 15% AMF Diet (1% Chol, 0.5% NaChol)
Research use:

Atherogenic diets are able to induce varied degrees of NASH with increased hepatic inflammation with early fibrosis observed after ten weeks of feeding. However, the metabolic profile typical in human NASH (obesity with insulin resistance) is not recapitulated in this model with animals typically maintaining similar body weights as control fed groups without the development of metabolic syndrome.

研究用途：

致動脈粥樣硬化飲食能夠誘導不同程度的 NASH，并在喂養 10 周后觀察到肝臟炎癥增加和早期纖維化。然而，人類 NASH（伴有胰島素抵抗的肥胖）中典型的代謝特征在該模型中沒有被概括，動物通常保持與對照組相似的體重，而沒有代謝綜合征的發展。

Select References: 參考文獻

Nishina, P.M., J. Verstuyft, and B. Paigen, Synthetic low and high fat diets for the study of atherosclerosis in the mouse. J Lipid Res, 1990. 31(5): p. 859-69. www.ncbi.nlm.nih.gov/pubmed/2380634

Kamari, Y., et al., Lack of interleukin-1alpha or interleukin-1beta inhibits transformation of steatosis to steatohepatitis and liver fibrosis in hypercholesterolemic mice. J Hepatol, 2011. 55(5): p. 1086-94. www.ncbi.nlm.nih.gov/pubmed/21354232

Kim, D.G., et al., Non-alcoholic fatty liver disease induces signs of Alzheimer’s disease (AD) in wild-type mice and accelerates pathological signs of AD in an AD model. J Neuroinflammation, 2016. 13: p. 1.
www.ncbi.nlm.nih.gov/pubmed/26728181

Madrigal-Perez, V.M., et al., Preclinical analysis of nonsteroidal anti-inflammatory drug usefulness for the simultaneous prevention of steatohepatitis, atherosclerosis and hyperlipidemia. Int J Clin Exp Med, 2015. 8(12): p. 22477-83. www.ncbi.nlm.nih.gov/pubmed/26885230

Savransky, V., et al., Chronic intermittent hypoxia causes hepatitis in a mouse model of diet-induced fatty liver. Am J Physiol Gastrointest Liver Physiol, 2007. 293(4): p. G871-7. www.ncbi.nlm.nih.gov/pubmed/17690174
- TD.94149 Amino Acid Control DietMethionine and choline deficient (MCD) diets are amino acid defined rodent diets deficient in methionine and choline, high in sucrose (>40% by weight) with ~10% corn oil by weight. Methionine and choline deficiency decreases fat oxidation and export of fat from the liver. Dietary sucrose is necessary for hepatic lipid accumulation and oxidation. The polyunsaturated fat in corn oil promotes hepatic lipid oxidation.
  蛋氨酸/膽堿缺乏 (MCD) 飲食
  蛋氨酸和膽堿缺乏 (MCD) 飲食是氨基酸定義的嚙齒動物飲食，缺乏蛋氨酸和膽堿，蔗糖含量高（> 40% 重量）和約 10% 玉米油重量。蛋氨酸和膽堿缺乏會降低脂肪氧化和脂肪從肝臟的輸出。膳食蔗糖是肝臟脂質積累和氧化所必需的。玉米油中的多不飽和脂肪促進肝臟脂質氧化。
  
  Example:
  - TD.90262 Methionine/Choline Deficient Diet
    
    Methionine/choline deficient (MCD) diets
  Control:
Research use:
Steatosis, increased serum alanine aminotransferase (ALT), inflammation, and hepatic fat oxidation has been observed within three weeks of feeding the MCD diet with fibrosis development after six weeks. This dietary model does not produce metabolic syndrome (an aspect of NASH in human models) and progressive weight loss (up to 40%) is associated with the MCD diet feeding.
研究用途：
在喂食 MCD 飲食的三周內觀察到脂肪變性、血清丙氨酸氨基轉移酶 (ALT) 升高、炎癥和肝臟脂肪氧化，六周后出現纖維化。這種飲食模型不會產生代謝綜合征（人體模型中 NASH 的一個方面），并且進行性體重減輕（高達 40%）與 MCD 飲食喂養有關。
Select References: 參考文獻

Pickens, M.K., et al., Dietary sucrose is essential to the development of liver injury in the MCD model of steatohepatitis. J Lipid Res, 2009. 50(10):2072-82. www.ncbi.nlm.nih.gov/pubmed/19295183

Li, Z.Z., et al., Hepatic lipid partitioning and liver damage in nonalcoholic fatty liver disease: role of stearoyl-CoA desaturase. J Biol Chem, 2009. 284(9): p. 5637-44. www.ncbi.nlm.nih.gov/pubmed/19119140

Lee, G.S., et al., Polyunsaturated fat in the methionine-choline-deficient diet influences hepatic inflammation but not hepatocellular injury. J Lipid Res, 2007. 48(8): p. 1885-96. www.ncbi.nlm.nih.gov/pubmed/17526933

Vetelainen, R., A. van Vliet, and T.M. van Gulik, Essential pathogenic and metabolic differences in steatosis induced by choline or methione-choline deficient diets in a rat model. J Gastroenterol Hepatol, 2007. 22(9): p. 1526-33. www.ncbi.nlm.nih.gov/pubmed/17716355

Leclercq, I.A., et al., Intrahepatic insulin resistance in a murine model of steatohepatitis: effect of PPARgamma agonist pioglitazone. Lab Invest, 2007. 87(1): p. 56-65. www.ncbi.nlm.nih.gov/pubmed/17075577

Kashireddy, P.R. and M.S. Rao, Sex differences in choline-deficient diet-induced steatohepatitis in mice. Exp Biol Med (Maywood), 2004. 229(2): p. 158-62. www.ncbi.nlm.nih.gov/pubmed/14734794

Dixon, L.J., et al., Caspase-1-mediated regulation of fibrogenesis in diet-induced steatohepatitis. Lab Invest, 2012. 92(5): p. 713-23. www.ncbi.nlm.nih.gov/pubmed/2241106
Emerging NASH models

Dietary models of NAFLD/NASH continue to evolve with the goal of more accurately recapitulating both the metabolic and hepatic symptoms of human disease. Commonly researchers are studying the synergistic effects of various NASH dietary features to accelerate progression of the model and severity of liver disease.

新興的 NASH 模型
NAFLD/NASH 的飲食模型繼續發展，目標是更準確地概括人類疾病的代謝和肝臟癥狀。通常，研究人員正在研究各種 NASH 飲食特征的協同效應，以加速模型的進展和肝病的嚴重程度。
Control diets

The choice of control diet is dependent on the specific research goal. Many researchers choose to compare their NAFLD/NASH diet-fed animals to animals fed a natural ingredient, grain-based diet (also referred to as standard diet or chow). These diets differ in the source and level of nutrients as well as in the presence of non-nutritive factors (such as phytates or phytoestrogens).

Depending on what your main comparisons are, it may be suitable to have a grain-based diet as your control/reference group. However, making such comparisons limits inferences to dietary patterns versus a specific dietary component. In some cases, such as those studies feeding amino acid defined diets like the MCD model, a matched control diet is recommended given the very different formulations and protein sources of grain-based diets.

When making inferences about specific nutrients within the diet an ingredient matched, low fat control diet may be necessary. There are many options with different levels and types of fat in addition to different types of carbohydrate ranging from sucrose (highly refined and digestible) to corn starch (refined, but more complex) to resistant starch (refined, but not fully digestible).

A very basic purified control diet would be AIN-93M TD.94048 or AIN-93G TD.94045 . AIN-93 diets have a moderate amount of sucrose at ~10% with fat from soybean oil providing a healthy fatty acid profile. Learn more about AIN diet formulas.

Contact a nutritionist for an additional information and control diet recommendations.

控制飲食
控制飲食的選擇取決于具體的研究目標。許多研究人員選擇將他們的 NAFLD/NASH 飲食喂養動物與喂養天然成分、谷物飲食（也稱為標準飲食或食物）的動物進行比較。這些飲食的營養來源和水平以及非營養因素（如植酸鹽或植物雌激素）的存在不同。

根據您的主要比較，以谷物為主的飲食作為您的對照組/參考組可能是合適的。然而，進行這樣的比較限制了對飲食模式與特定飲食成分的推斷。在某些情況下，例如那些飼喂氨基酸定義飲食（如 MCD 模型）的研究，鑒于谷物飲食的配方和蛋白質來源非常不同，建議使用匹配的對照飲食。

當推斷飲食中的特定營養成分匹配時，可能需要低脂肪控制飲食。除了不同類型的碳水化合物，從蔗糖（高度精制和易消化）到玉米淀粉（精制，但更復雜）再到抗性淀粉（精制，但不能完全消化），還有許多脂肪含量和類型不同的選擇。

一個非?；镜募兓瘜φ诊嬍呈?AIN-93M TD.94048 或 AIN-93G TD.94045。 AIN-93 日糧含有適量的蔗糖，約為 10%，而大豆油中的脂肪提供了健康的脂肪酸成分。了解更多關于 AIN 飲食配方的信息。

聯系營養師以獲取更多信息和控制飲食建議。

Live chatLive chat ?

圖片僅供參考,請以實物為準。

Harlan 強力霉素飲食Doxycycline diets

發表于2022年1月13日由上海金畔生物科技有限公司

Harlan 強力霉素飲食Doxycycline diets

Harlan Teklad動物飼料
歡迎新老客戶訪問Harlan官網或者咨詢上海金畔生物獲取更多詳細資料。

Doxycycline diets

Envigo Teklad diets makes a variety of doxycycline containing diets for your Tet regulated systems. TD.01306 with doxycycline hyclate added at 625 mg/kg to Teklad global 2018 rodent diet is stocked and can typically ship within 1-2 days (non-irradiated) or 1-2 weeks (irradiated). Diets are easily customizable. Custom specifications include: base diet, concentration of doxycycline, and food coloring. Contact us to discuss your needs if you don’t find a suitable option below.

See our lists of doxycycline references and phytoestrogen references?for additional information.

Envigo Teklad 飲食為您的春節調節系統制作各種含有強力霉素的飲食。 TD.01306 在 Teklad 全球 2018 年嚙齒動物飲食中添加了 625 mg/kg 的鹽酸多西環素，通?？梢栽?1-2 天（未輻照）或 1-2 周（輻照）內發貨。飲食很容易定制。定制規格包括：基礎飲食、強力霉素濃度和食用色素。如果您在下面找不到合適的選項，請聯系我們討論您的需求。

有關更多信息，請參閱我們的強力霉素參考和植物雌激素參考列表。

order today

18301939375

上海金畔生物科技有限公司

Formula examples with Teklad global 2018 base diet

Doxycycline hyclate¹ USP (mg/kg diet)	Natural color	Alternatives with food coloring
46	TD.10483	TD.180327 Red TD.120240 Blue
100	TD.04233	TD.130840 Blue
200	TD.00502	TD.180625 Red TD.150431 Blue TD.04104 Green
625	TD.01306 stocked²	TD.08541 Red TD.120769 Blue TD.09628 Green
1000	TD.05298	TD.06294 Red TD.120658 Blue
2000	TD.05512	TD.140011 Red TD.09633 Green
6000	TD.01533

¹ Doxycycline hyclate is ~87% doxycycline
² TD.01306 typically ships within 2 days non-irradiated or 2 weeks with irradiation

1 鹽酸強力霉素約為 87% 強力霉素
2 TD.01306 通常在未經輻照的 2 天內或輻照的 2 周內發貨

Considerations when choosing a base diet

Teklad Global Rodent Diets^? like 2018 do not contain alfalfa and are thus ideal if your work requires an alfalfa-free diet for fluorescent imaging, If further imaging clarity is needed, we can make purified diets with doxycycline.
Teklad global 2018 (base diet in examples above) contains a moderate amount of soybean meal, a source of phytoestrogens. The table below includes several options of minimal phytoestrogen diets with the most common doxycycline concentration of 625 mg/kg diet. For other concentrations contact us. For additional phytoestrogen information, see our list of phytoestrogen references.

選擇基礎飲食時的注意事項

Teklad Global Rodent Diets?（如 2018 年）不含苜蓿，因此如果您的工作需要無苜蓿的飲食進行熒光成像，則非常理想。如果需要進一步的成像清晰度，我們可以使用強力霉素制作純化飲食。
Teklad global 2018（上述示例中的基礎飲食）含有適量的豆粕，這是植物雌激素的來源。下表包括幾種最低植物雌激素飲食選擇，最常見的強力霉素濃度為 625 毫克/千克飲食。如需其他濃度，請聯系我們。有關其他植物雌激素信息，請參閱我們的植物雌激素參考列表。

Examples of minimal phytoestrogen global rodent diets with doxycycline hyclate¹ at 625 mg/kg diet

使用 625 mg/kg 飲食的多西環素 hyclate1 的最低植物雌激素全球嚙齒動物飲食示例

Base diet	Natural color	Alternatives with food coloring mg per kg diet
2014	TD.00426	TD.07382 Red TD.07573 Yellow
2016	TD.02503	TD.140263 Blue TD.170364v Red
2019	TD.09651	TD.09761 Green
2020	TD.110720	TD.130141 Red

¹ Doxycycline hyclate is ~87% doxycycline

1 鹽酸強力霉素約為 87% 強力霉素

Advantages of diet delivery of doxycycline

Typically the same doxycycline concentration can be used in diet as in water. Lower concentrations of doxycycline may be adequate with 625 mg/kg diet being the most common concentration.
Diet offers protection from light. Water may have to be given in dark-colored or foil-wrapped bottles
Diet is typically changed once per week. Water may need to be changed every few days
Diet delivery may also reduce risk of dehydration and preclude the need to give with sucrose (Cawthorne et. al. 2007)

多西環素飲食輸送的優點

通常，可以在飲食中使用與水中相同濃度的強力霉素。較低濃度的強力霉素可能就足夠了，最常見的濃度為 625 mg/kg 飲食。
飲食提供避光保護。水可能必須裝在深色或鋁箔包裝的瓶子中
飲食通常每周改變一次。可能需要每隔幾天換一次水
飲食還可以降低脫水的風險，并排除給予蔗糖的需要（Cawthorne et. al. 2007）

Key planning information

Minimum order quantity is 3 kg, sufficient for feeding ~20 mice for one month
Store diet refrigerated and plan to use within six months
Typical lead time is two weeks (four weeks if irradiated)
TD.01306 typically ships within 2 days non-irradiated or 2 weeks with irradiation
Irradiation (20 – 50 kGy) is optional, and must be requested at time of order

主要規劃信息

最小起訂量為 3 公斤，足夠喂食約 20 只老鼠一個月
將飲食冷藏并計劃在六個月內使用
典型的交貨時間是兩周（如果受到輻照，則為四個星期）
TD.01306 通常在未經輻照的 2 天內或輻照的 2 周內發貨
輻照 (20 – 50 kGy) 是可選的，必須在訂購時提出要求

Safe handling of doxycycline diets

Doxycycline is a tetracycline class antibiotic.
Usual dose for therapeutic effects in humans is 100-200 mg/day.
Doxycycline diets for rodents typically contain ~2 mg/pellet.
Accidental doxycycline exposure can be minimized by using typical lab precautions of lab coat, gloves, and mask when handling the diet.
Your chemical safety department should be contacted for additional institution specific guidelines for handling and disposal of doxycycline containing diets.

安全處理強力霉素飲食

強力霉素是一種四環素類抗生素。
人類治療效果的常用劑量為 100-200 毫克/天。
嚙齒動物的強力霉素飲食通常含有約 2 毫克/粒。
通過在處理飲食時使用實驗室外套、手套和面罩等典型的實驗室預防措施，可以最大限度地減少意外的強力霉素暴露。
應聯系您的化學安全部門以獲取有關處理和處置含有多西環素飲食的其他機構特定指南。

See our list of doxycycline references?for additional information.

有關更多信息，請參閱我們的多西環素參考文獻列表。

如果您有具體需求，請聯系我們，我們可以推薦現有產品代碼或創建新配方。

Technical resources? 技術文章

Live chatLive chat ?

強力霉素飲食圖片僅供參考,請以實物為準。

飼養環境

實驗大鼠的選擇

高脂飼料配方

建模周期

總結

Rodent diets

order today

Products

Technical resources??技術文章

Diet ingredients

order today

Vitamin adjusted diets

order today

Mineral adjusted diets

order today

Protein adjusted diets

order today

Teklad diet selector

Amino acid defined

order today

Fat/lipid adjusted

order today

Atherogenic

order today

“Western” purified atherogenic diet with added cholesterol and cholate source*

Hybrid high fat diets with added cholesterol and cholate source*

Standard diets with added cholesterol

Diets for additional animal models of atherosclerosis

Diet induced obesity

order today

Commonly-used diet-induced obesity (DIO) Teklad rodent diets with 55-60% of calories from fat

Commonly-used diet-induced obesity (DIO) Teklad rodent diets with 40-45% of calories from fat

Diets for diet-induced diabetes

Ingredient matched, low-fat DIO control diets

Carbohydrate adjusted

order today

NaCl adjusted (natural ingredient)

order today

NAFLD and NASH

order today

Diet options for inducing obesity, metabolic syndrome and mild NAFLD/NASH

Diet options for inducing more severe hepatic NAFLD/NASH without obesity or metabolic syndrome

Diets inducing obesity, metabolic syndrome and mild NAFLD/NASH

Western and Fast Food diets with milkfat and cholesterol

The ALIOS model: western diet with trans-fat

FPC diet: fructose, palmitate, cholesterol and trans-fat diet

High fat diets

Diets to induce severe hepatic NAFLD/NASH without obesity or metabolic

Atherogenic diets high in fat, cholesterol, and cholate

Methionine/choline deficient (MCD) diets

Emerging NASH models

Control diets

Doxycycline diets

order today

Formula examples with Teklad global 2018 base diet

Examples of minimal phytoestrogen global rodent diets with doxycycline hyclate1 at 625 mg/kg diet

Technical resources? 技術文章

Examples of minimal phytoestrogen global rodent diets with doxycycline hyclate¹ at 625 mg/kg diet