分類目錄歸檔：Vector lab

Vectorlabs代理-Fluorescein Maleimide, 熒光素馬來酰亞胺12 mg

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Vectorlabs代理-Fluorescein Maleimide, 熒光素馬來酰亞胺12 mg

Vector Lab代理
上海金畔生物代理Vector Lab品牌產品，歡迎訪問Vector Labs官網或者咨詢我們獲取相關產品信息。
Vector Laboratories主要產品類別
ABC免疫組化試劑盒、ImmPRESS聚合物免疫組化試劑盒、防熒光淬滅封片劑、二抗、酶底物、親和素/鏈霉親和素-酶結合物、封閉試劑。

Fluorescein Maleimide, 12 mg

品牌：Vectorlabs
產品貨號：SP-1502-12
規格：12 mg
產品詳情
* 以下信息僅供參考，詳情請以原廠網站為準

  • 產品名稱:Fluorescein Maleimide, 12 mg

    別名:熒光素標記的馬來酰亞胺

    產品描述:

    Fluorescein Maleimide is designed for fluoresceinating proteins, nucleic acids or other molecules containing one or more thiol groups. Molecules to be labeled with Fluorescein Maleimide require free thiol groups. In the case of proteins, Fluorescein Maleimide will react with exposed cysteine residues. Alternatively, free thiols may be generated by reducing disulfide bonds or by modifying other reactive groups such as primary amines with compounds like Traut’s reagent (2-iminothiolane).For labeling nucleic acids, thiols can be introduced into DNA, RNA, or oligonucleotides using the FastTag?, 5′ EndTag™ or 3′ EndTag™ nucleic acid labeling systems.This fluorescent label can be directly detected or used as a hapten and detected with our high affinity antibody reagents. (Excitation maximum at 495 nm; emission maximum at 515 nm).

Vectorlabs代理-Succinylated Wheat Germ Agglutinin (WGA), Agarose bound, 5 ml

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Vectorlabs代理-Succinylated Wheat Germ Agglutinin (WGA), Agarose bound, 5 ml

Vector Lab代理
上海金畔生物代理Vector Lab品牌產品，歡迎訪問Vector Labs官網或者咨詢我們獲取相關產品信息。
Vector Laboratories主要產品類別
ABC免疫組化試劑盒、ImmPRESS聚合物免疫組化試劑盒、防熒光淬滅封片劑、二抗、酶底物、親和素/鏈霉親和素-酶結合物、封閉試劑。

Succinylated Wheat Germ Agglutinin (WGA), Agarose bound, 5 ml

品牌：Vectorlabs

產品貨號：AL-1023S-5

規格：5 ml

產品詳情

* 以下信息僅供參考，詳情請以原廠網站為準

產品名稱:Succinylated Wheat Germ Agglutinin (WGA), Agarose bound, 5 ml

別名:琥珀?；←溑哐磕?，瓊脂糖交聯

產品描述:

Wheat germ agglutinin (WGA) succinylated has been reported to have properties distinct from the native lectin. Evidence suggests that Succinylated Wheat Germ agglutinin does not bind to sialic acid residues, unlike the native form, but retains its specificity toward N-acetylglucosamine. Using conjugates of the native lectin and the succinylated form can provide a system to distinguish between sialylated glycoconjugates and those containing only N-acetylglucosamine structures.Agarose bound*, succinylated WGA is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2×107 daltons are used as the solid-phase matrix to which the lectins are covalently coupled. The attachment of the lectins to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins that might result in nonspecific ionic or hydrophobic interactions. The technique we have developed to couple lectins to agarose beads inserts a hydrophilic spacer arm between the lectin and the matrix.This coupling method provides several advantages over the traditional cyanogen bromide procedure:Maximum carbohydrate binding activity of the coupled lectins is retainedLinkage is stable over a range of pH valuesConjugated proteins are not leached off the beads by Tris or other routinely used buffersNo residual charges are present after conjugation. This minimizes non-specific binding to the matrix.Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins.Inhibiting/Eluting Sugar: Chitin Hydrolysate (Cat. No. SP-0090) or 500 mM N-acetylglucosamine with salt and/or acid elution generally required*3 mg lectin/ml gel

據報道，琥珀?；男←溑哐磕?(WGA) 具有不同于天然凝集素的特性。有證據表明，與天然形式不同，琥珀?；←溑哐磕夭慌c唾液酸殘基結合，但保留了其對 N-乙酰氨基葡萄糖的特異性。使用天然凝集素和琥珀?；问降木Y合物可以提供一個系統來區分唾液酸化糖綴合物和僅含有 N-乙酰氨基葡萄糖結構的糖綴合物。瓊脂糖結合*，琥珀?；?WGA 是使用我們的親和純化凝集素制備的。具有約 2×107 道爾頓分子量排除極限的熱穩定、交聯的 4% 瓊脂糖珠用作凝集素共價偶聯的固相基質。凝集素與珠子的連接受到仔細控制，以保持凝集素活性并最大限度地減少可能導致非特異性離子或疏水相互作用的結合凝集素的構象變化。我們開發的將凝集素與瓊脂糖珠偶聯的技術在凝集素和基質之間插入了一個親水性間隔臂。這種偶聯方法與傳統的溴化氰方法相比具有幾個優點：保持偶聯凝集素的最大碳水化合物結合活性pH 值范圍 結合的蛋白質不會被 Tris 或其他常規使用的緩沖液從珠子上浸出 結合后不存在殘留電荷。這最大限度地減少了與基質的非特異性結合。我們的瓊脂糖結合凝集素以每毫升沉降珠子的恒定凝集素濃度提供。選擇每種凝集素的濃度以實現珠中每毫克凝集素的最高糖綴合物結合能力。使用已知結合凝集素的糖蛋白測試每批的結合能力。這為用戶提供了指導并確保了我們瓊脂糖結合凝集素的質量。抑制/洗脫糖：甲殼素水解物（貨號 SP-0090）或 500 mM N-乙酰氨基葡萄糖，通常需要鹽和/或酸洗脫*3毫克凝集素/毫升凝膠

實驗應用:Glycobiology,Affinity Chromatography

推薦稀釋度:

Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting solution, Cat. No. ES-5100. Alternatively, 0.5 M N-Acetyl-D-Glucosamine (GlcNAc) can be used.For those glycoconjugates having very high affinity for WGA, it may be necessary to lower the pH of the eluting sugar solution to pH 3.0 with acetic acid and increase the concentration of GlcNAc. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.

使用前用緩沖液徹底清洗凝膠，以去除為穩定凝集素而添加的糖。 用于洗脫與該瓊脂糖凝集素結合的糖綴合物的推薦產品：糖蛋白洗脫溶液，Cat. 編號 ES-5100。 或者，可以使用 0.5 M N-乙?；?D-氨基葡萄糖 (GlcNAc)。對于那些對 WGA 具有非常高親和力的糖綴合物，可能需要用乙酸將洗脫糖溶液的 pH 值降低到 3.0 并增加 GlcNAc 的濃度。 使用后，用幾柱體積的緩沖鹽水清洗凝膠，然后將凝膠重懸于含有 0.08% 疊氮化鈉的緩沖鹽水中保存。

偶聯:Agarose

存儲溶液:10 mM HEPES, pH 7.5, 0.15 M NaCl, 20 mM GlcNAc, 0.08% sodium azide

樣本類型:10 mM HEPES, pH 7.5, 0.15 M NaCl, 20 mM GlcNAc, 0.08% sodium azide

Vectorlabs 抗體-Goat Anti-Human IgG Antibody (H+L), Peroxidase, 1 mg

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Vectorlabs 抗體-Goat Anti-Human IgG Antibody (H+L), Peroxidase, 1 mg

Vector Lab代理
上海金畔生物代理Vector Lab品牌產品，歡迎訪問Vector Labs官網或者咨詢我們獲取相關產品信息。
Vector Laboratories主要產品類別
ABC免疫組化試劑盒、ImmPRESS聚合物免疫組化試劑盒、防熒光淬滅封片劑、二抗、酶底物、親和素/鏈霉親和素-酶結合物、封閉試劑。Goat Anti-Human IgG Antibody (H+L), Peroxidase, 1 mg

  • 產品名稱:Goat Anti-Human IgG Antibody (H+L), Peroxidase, 1 mg
  • 別名:山羊抗人IgG (H+L)抗體，HRP標記
  • 產品描述:
    Goat Anti-Human IgG Antibody is purified by affinity chromatography and adsorbed to remove any cross-reactivities that are likely to interfere with specific labeling. This antibody is then cross-linked with the highest specific activity horseradish peroxidase. A proprietary procedure for conjugation allows the maximum preservation of enzyme activity and antibody specificity. This antibody is subjected to rigorous quality control assays and can be used for tissue staining, ELISAs, and blots.
  • 反應種屬:Human
  • 宿主來源:Goat
  • 實驗應用:Immunohistochemistry / Immunocytochemistry,Blotting Applications,Elispot,ELISAs
  • 推薦稀釋度:
    Recommended diluent for microtitration plate assays: 10 mM phosphate, 0.15 M NaCl, pH 7.5, 0.1% Tween 20.Working concentration of HRP-anti human IgG is 0.5-5.0μg/ml.
  • 偶聯:Peroxidase
  • 產品濃度:1 mg active conjugate/ml
  • 產品形式:Concentrate
  • 存儲溶液:10 mM HEPES, 0.15 M NaCl, pH 7.5, preservative
  • 產地:美國
  • 樣本類型:10 mM HEPES, 0.15 M NaCl, pH 7.5, preservative

Vectorlabs 蘇木精/蘇木素復染試劑-Hematoxylin Counterstain, 500 ml

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Vectorlabs 蘇木精/蘇木素復染試劑-Hematoxylin Counterstain, 500 ml

Vector Lab代理
上海金畔生物代理Vector Lab品牌產品，歡迎訪問Vector Labs官網或者咨詢我們獲取相關產品信息。
Vector Laboratories主要產品類別
ABC免疫組化試劑盒、ImmPRESS聚合物免疫組化試劑盒、防熒光淬滅封片劑、二抗、酶底物、親和素/鏈霉親和素-酶結合物、封閉試劑。

Hematoxylin Counterstain, 500 ml

品牌：Vectorlabs
產品貨號：H-3401-500
規格：500 ml
產品詳情
* 以下信息僅供參考，詳情請以原廠網站為準
  • 產品名稱:
    Hematoxylin Counterstain, 500 ml
  • 別名:蘇木精/蘇木素復染試劑
  • 產品描述:
    Vector’s Hematoxylin counterstain produces blue stained nuclei with crisp detail. This commonly utilized counterstain is based on Gill s formulation and is mercury free. As this is a progressive stain formula, the intensity of nuclear staining can be adjusted to achieve the most desirable results either manually or in an automated instrument. This counterstain provides excellent color contrast with most commonly used peroxidase and alkaline phosphatase substrates and is suitable for use with non-aqueous and aqueous mountants.
  • 實驗應用:
    Immunohistochemistry / Immunocytochemistry,In situ hybridization
  • 產品形式:
    Ready-to-Use
  • 產地:
    美國

Vectorlabs TEXAS RED標記的馬來酰亞胺-Texas Red Maleimide, 3.6 mg

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Vectorlabs TEXAS RED標記的馬來酰亞胺-Texas Red Maleimide, 3.6 mg

Vector Lab代理
上海金畔生物代理Vector Lab品牌產品，歡迎訪問Vector Labs官網或者咨詢我們獲取相關產品信息。
Vector Laboratories主要產品類別
ABC免疫組化試劑盒、ImmPRESS聚合物免疫組化試劑盒、防熒光淬滅封片劑、二抗、酶底物、親和素/鏈霉親和素-酶結合物、封閉試劑。

Texas Red? Maleimide, 3.6 mg

品牌：Vectorlabs
產品貨號：SP-1505-3.6
規格：3.6 mg
產品詳情
* 以下信息僅供參考，詳情請以原廠網站為準
  • 產品名稱:Texas Red Maleimide, 3.6 mg
  • 別名:TEXAS RED標記的馬來酰亞胺
  • 產品描述:
    Texas Red Maleimide is designed for fluorescent labeling of proteins, nucleic acids or other molecules containing one or more thiol groups. Molecules to be labeled with Texas Red? Maleimide require free thiol groups. In the case of proteins, Texas Red Maleimide will react with exposed cysteine residues. Alternatively, free thiols may be generated by reducing disulfide bonds or by modifying other reactive groups such as primary amines with compounds like Traut’s reagent (2-iminothiolane).For labeling nucleic acids, thiols can be introduced into DNA, RNA, or oligonucleotides using the FastTag?, 5′ EndTag™ or 3′ EndTag™ nucleic acid labeling systems.This fluorescent label is a high quantum yield rhodamine derivative that can be directly visualized. The label can also be detected, and the signal amplified, using our affinity-purified antibody conjugates to rhodamine. (Excitation maximum at 595 nm; emission maximum at 615 nm).
  • 預期分子量:728
  • 產地:美國

Vectorlabs 花生凝集素，Cy3標記-Peanut Agglutinin (PNA), CY3, 1 mg

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Vectorlabs 花生凝集素，Cy3標記-Peanut Agglutinin (PNA), CY3, 1 mg

Vector Lab代理
上海金畔生物代理Vector Lab品牌產品，歡迎訪問Vector Labs官網或者咨詢我們獲取相關產品信息。
Vector Laboratories主要產品類別
ABC免疫組化試劑盒、ImmPRESS聚合物免疫組化試劑盒、防熒光淬滅封片劑、二抗、酶底物、親和素/鏈霉親和素-酶結合物、封閉試劑。

Peanut Agglutinin (PNA), CY3, 1 mg

品牌：Vectorlabs
產品貨號：CL-1073-1
規格：1 mg
產品詳情
* 以下信息僅供參考，詳情請以原廠網站為準

產品名稱:Peanut Agglutinin (PNA), CY?3, 1 mg

別名:花生凝集素，Cy3標記

產品描述:

Cy3 PEANUT AGGLUTININ PNA binds preferentially to the T-antigen, a galactosyl (β-1,3) N-acetylgalactosamine structure present in many glycoconjugates such as M and N blood groups, gangliosides, and many other soluble and membrane-associated glycoproteins and glycolipids. With certain exceptions, the receptor sequence for PNA is normally sialylated which prevents the lectin from binding to its receptor oligosaccharide (see Jacalin). Even sialic acid which is not bound directly to the receptor sugars may inhibit binding. The presence of calcium ions in diluents can enhance the binding of PNA to receptors, possibly by neutralizing the negative charges on sialic acid residues adjacent to the receptor sequence.Cy3 labeled Peanut agglutinin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.Excitation: 550 nmEmission: 570 nmColor: Red

實驗應用:Immunofluorescence,Glycobiology

推薦稀釋度:The recommended concentration range for use is 5-20μg/ml.

偶聯:Cy3

產品濃度:1 mg active conjugate/ml

存儲溶液:10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, and a proprietary stabilizer.

產地:美國

樣本類型:10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, and a proprietary stabilizer.

Vectorlabs Ulex Europaeus Agglutinin I (UEA I), Agarose bound, 2 ml

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Vectorlabs代理-Ulex Europaeus Agglutinin I (UEA I), Agarose bound, 2 ml

Vector Lab代理
上海金畔生物代理Vector Lab品牌產品，歡迎訪問Vector Labs官網或者咨詢我們獲取相關產品信息。
Vector Laboratories主要產品類別
ABC免疫組化試劑盒、ImmPRESS聚合物免疫組化試劑盒、防熒光淬滅封片劑、二抗、酶底物、親和素/鏈霉親和素-酶結合物、封閉試劑。

Ulex Europaeus Agglutinin I (UEA I), Agarose bound, 2 ml

品牌：Vectorlabs
產品貨號：AL-1063-2
規格：2 ml
產品詳情
* 以下信息僅供參考，詳情請以原廠網站為準
  • 產品名稱:
    Ulex Europaeus Agglutinin I (UEA I), Agarose bound, 2 ml
  • 別名:
    荊豆凝集素I，瓊脂糖交聯
  • 產品描述:
  • Ulex europaeus agglutinin I (UEA I) binds to many glycoproteins and glycolipids containing α-linked fucose residues, such as ABO blood group glycoconjugates. This lectin preferentially binds blood group O cells and has been used to determine secretor status. It has been established as an excellent marker for human endothelial cells.Agarose bound* Ulex europaeus agglutinin is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2×107 daltons are used as the solid-phase matrix to which the lectins are covalently coupled. The attachment of the lectins to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins that might result in nonspecific ionic or hydrophobic interactions. The technique we have developed to couple lectins to agarose beads inserts a hydrophilic spacer arm between the lectin and the matrix.This coupling method provides several advantages over the traditional cyanogen bromide procedure:Maximum carbohydrate binding activity of the coupled lectins is retainedLinkage is stable over a range of pH valuesConjugated proteins are not leached off the beads by Tris or other routinely used buffersNo residual charges are present after conjugation. This minimizes non-specific binding to the matrix.Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins. Inhibiting/Eluting Sugar: 50 mM – 100 mM L-fucose*2 mg lectin/ml gel
  • 實驗應用:
    Glycobiology,Affinity Chromatography
  • 推薦稀釋度:
  • Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-3100. Alternatively, 100 mM L-fucose in buffered saline can be used. After use, wash the gel with several column volumes of buffered saline then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
  • 偶聯:
    Agarose
  • 存儲溶液:
  • 10 mM HEPES, pH 7.5, 0.15 M NaCl, 10 mM fucose, 0.1 mM CaCl2, 0.08% sodium azide
  • 產地:
    美國
  • 樣本類型:
  • 10 mM HEPES, pH 7.5, 0.15 M NaCl, 10 mM fucose, 0.1 mM CaCl2, 0.08% sodium azide

Vectorlabs Goat Anti-Rat IgG Antibody (H+L), Peroxidase, 1 mg

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Vectorlabs Goat Anti-Rat IgG Antibody (H+L), Peroxidase, 1 mg

Vector Lab代理
上海金畔生物代理Vector Lab品牌產品，歡迎訪問Vector Labs官網或者咨詢我們獲取相關產品信息。
Vector Laboratories主要產品類別
ABC免疫組化試劑盒、ImmPRESS聚合物免疫組化試劑盒、防熒光淬滅封片劑、二抗、酶底物、親和素/鏈霉親和素-酶結合物、封閉試劑。

Goat Anti-Rat IgG Antibody (H+L), Peroxidase, 1 mg

品牌：Vectorlabs
產品貨號：PI-9400-1
規格：1 mg
產品詳情
* 以下信息僅供參考，詳情請以原廠網站為準
  • 產品名稱:
    Goat Anti-Rat IgG Antibody (H+L), Peroxidase, 1 mg
  • 別名:
    山羊抗大鼠IgG（H+L）抗體，HRP標記
  • 產品描述:
    Goat Anti-Rat IgG Antibody is purified by affinity chromatography and adsorbed to remove any cross-reactivities that are likely to interfere with specific labeling. This antibody is then cross-linked with the highest specific activity horseradish peroxidase. A proprietary procedure for conjugation allows the maximum preservation of enzyme activity and antibody specificity. This antibody is subjected to rigorous quality control assays and can be used for tissue staining, ELISAs, and blots.Peroxidase Goat Anti-Rat IgG is supplied in solution. With some exceptions, working concentration of HRP Anti-Rat IgG is 0.5-5.0 ug/ml.
  • 反應種屬:
    Rat
  • 宿主來源:
    Goat
  • 實驗應用:
  • Immunohistochemistry / Immunocytochemistry,Blotting Applications,Elispot,ELISAs
  • 推薦稀釋度:
  • Recommended diluent for microtitration plate assays: 10 mM phosphate, 0.15 M NaCl, pH 7.5, 0.1% Tween 20.Working concentration of HRP-anti rat IgG is 0.5-5.0μg/ml.
  • 偶聯:
    Peroxidase
  • 產品濃度:
    1 mg active conjugate/ml
  • 產品形式:
    Concentrate
  • 存儲溶液:
    10 mM HEPES, 0.15 M NaCl, pH 7.5, preservative
  • 樣本類型:
    10 mM HEPES, 0.15 M NaCl, pH 7.5, preservative

Vectorlabs Cy5標記花生凝集素 Peanut Agglutinin (PNA), CY5, 1 mg

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Vectorlabs Cy5標記花生凝集素 Peanut Agglutinin (PNA), CY5, 1 mg

Vector Lab代理
上海金畔生物代理Vector Lab品牌產品，歡迎訪問Vector Labs官網或者咨詢我們獲取相關產品信息。
Vector Laboratories主要產品類別
ABC免疫組化試劑盒、ImmPRESS聚合物免疫組化試劑盒、防熒光淬滅封片劑、二抗、酶底物、親和素/鏈霉親和素-酶結合物、封閉試劑。Peanut Agglutinin (PNA), CY?5, 1 mg

品牌：Vectorlabs
產品貨號：CL-1075-1
規格：1 mg
產品詳情
* 以下信息僅供參考，詳情請以原廠網站為準
  • 產品名稱:
    Peanut Agglutinin (PNA), CY?5, 1 mg
  • 別名:
    花生凝集素，Cy5標記
  • 產品描述:
  • CY5 PEANUT AGGLUTININ PNA binds preferentially to the T-antigen, a galactosyl (β-1,3) N-acetylgalactosamine structure present in many glycoconjugates such as M and N blood groups, gangliosides, and many other soluble and membrane-associated glycoproteins and glycolipids. With certain exceptions, the receptor sequence for PNA is normally sialylated which prevents the lectin from binding to its receptor oligosaccharide (see Jacalin). Even sialic acid which is not bound directly to the receptor sugars may inhibit binding. The presence of calcium ions in diluents can enhance the binding of PNA to receptors, possibly by neutralizing the negative charges on sialic acid residues adjacent to the receptor sequence.Cy5 labeled Peanut agglutinin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.Excitation: 649 nmEmission: 670 nmColor: Far Red
  • 實驗應用:
    Immunofluorescence,Glycobiology
  • 推薦稀釋度:
    The recommended concentration range for use is 5-20μg/ml.
  • 偶聯:
    Cy5
  • 產品濃度:
    1 mg active conjugate/ml
  • 存儲溶液:
  • 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, and a proprietary stabilizer.
  • 樣本類型:
  • 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, and a proprietary stabilizer.

Vectorlabs 甲基綠復染試劑 Methyl Green Counterstain, 500 ml

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Vectorlabs 甲基綠復染試劑 Methyl Green Counterstain, 500 ml

Vector Lab代理
上海金畔生物代理Vector Lab品牌產品，歡迎訪問Vector Labs官網或者咨詢我們獲取相關產品信息。
Vector Laboratories主要產品類別
ABC免疫組化試劑盒、ImmPRESS聚合物免疫組化試劑盒、防熒光淬滅封片劑、二抗、酶底物、親和素/鏈霉親和素-酶結合物、封閉試劑。

Methyl Green Counterstain, 500 ml

品牌：Vectorlabs
產品貨號：H-3402-500
規格：500 ml
產品詳情
* 以下信息僅供參考，詳情請以原廠網站為準
  • 產品名稱:
    Methyl Green Counterstain, 500 ml
  • 別名:
    甲基綠復染試劑
  • 產品描述:
  • Vector’s Methyl Green counterstain cell nuclei light green. This superior formulation of methyl green is suitable for use with a wide range of enzyme substrates. Optimal tissue staining is achieved by using a simple, two-step procedure. This counterstain provides an excellent alternative in multiple labeling when hematoxylin obscures the substrate colors. This counterstain is suitable for use with non-aqueous mountants.
  • 實驗應用:
    Immunohistochemistry / Immunocytochemistry,In situ hybridization
  • 產品形式:
    Ready-to-Use

Vectorlabs代理-VECTABOND? Reagent, Tissue Section Adhesion, 7 ml

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Vector Lab代理
上海金畔生物代理Vector Lab品牌產品，歡迎訪問Vector Labs官網或者咨詢我們獲取相關產品信息。
Vector Laboratories主要產品類別
ABC免疫組化試劑盒、ImmPRESS聚合物免疫組化試劑盒、防熒光淬滅封片劑、二抗、酶底物、親和素/鏈霉親和素-酶結合物、封閉試劑。VECTABOND? Reagent, Tissue Section Adhesion, 7 ml

品牌： Vectorlabs
產品貨號： SP-1800-7
規格： 7 ml
產品詳情
* 以下信息僅供參考，詳情請以原廠網站為準

  • 產品名稱:
    VECTABOND? Reagent, Tissue Section Adhesion, 7 ml
  • 別名:
    組織切片粘附劑
  • 產品描述:
    VECTABOND Reagent is designed to significantly increase the adherence of both frozen and paraffin-embedded tissue sections and cell preparations to glass slides and coverslips. Tissue sections will remain attached even when subjected to the most extreme conditions, such as high temperature antigen unmasking techniques and in situ hybridization. VECTABOND™ Reagent treated slides can be stored for long periods. This product chemically modifies the glass to form a highly adherent surface. VECTABOND™ Reagent is provided as 7 ml of concentrate that dilutes to 350 ml of treatment solution, sufficient for at least 500 standard slides.
  • 反應種屬:
  • 宿主來源:
  • 實驗應用:
    Immunohistochemistry / Immunocytochemistry,Immunofluorescence,In situ hybridization
  • 靶標/特異性:
  • 同種型:
  • 推薦稀釋度:
    The VECTABOND?? Reagent for Tissue Section Adhesion is designed to significantly increase the adherence of both frozen and paraffin-embedded tissue sections and cell preparations to glass slides and coverslips. It chemically modifies the surface of the glass with positive charges to form a highly adherent surface.
  • 免疫原:
  • 免疫原種屬:
  • 克隆性:
  • 克隆號:
  • 純化方式:
  • 偶聯:
  • 產品濃度:
  • 表達系統:
  • 陽性對照:
  • 保存溫度:
  • 運輸溫度:
  • 細胞定位:
  • 預期分子量:
  • 產品形式:
  • 存儲溶液:
  • 有效期:
    效期最長8~10個月
  • 產地:
    美國
  • 外觀:
  • 生產商:
  • 功能與背景:
  • URL:
  • 靈敏度:
  • 檢測范圍:
  • 檢測方法:
  • 樣本類型:
  • 產品特點:
  • 純度:
  • 生物活性:
  • 內毒素水平:
  • 來源:
  • 表達方式:
  • Accession #:
  • CAS號:
  • 分子式:
  • 溶解度:
  • PubChem CID:

Vectorlabs代理-Peanut Agglutinin (PNA), Agarose bound, 2 ml

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Vector Lab代理
上海金畔生物代理Vector Lab品牌產品，歡迎訪問Vector Labs官網或者咨詢我們獲取相關產品信息。
Vector Laboratories主要產品類別
ABC免疫組化試劑盒、ImmPRESS聚合物免疫組化試劑盒、防熒光淬滅封片劑、二抗、酶底物、親和素/鏈霉親和素-酶結合物、封閉試劑。Peanut Agglutinin (PNA), Agarose bound, 2 ml

品牌： Vectorlabs
產品貨號： AL-1073-2
規格： 2 ml
產品詳情
* 以下信息僅供參考，詳情請以原廠網站為準

  • 產品名稱:
    Peanut Agglutinin (PNA), Agarose bound, 2 ml
  • 別名:
    花生凝集素，瓊脂糖交聯
  • 產品描述:
    Peanut agglutinin (PNA) binds preferentially to the T-antigen, a galactosyl (β-1,3) N-acetylgalactosamine structure present in many glycoconjugates such as M and N blood groups, gangliosides, and many other soluble and membrane-associated glycoproteins and glycolipids. With certain exceptions, the receptor sequence for PNA is normally sialylated which prevents the lectin from binding to its receptor oligosaccharide (see Jacalin). Even sialic acid which is not bound directly to the receptor sugars may inhibit binding. The presence of calcium ions in diluents can enhance the binding of PNA to receptors, possibly by neutralizing the negative charges on sialic acid residues adjacent to the receptor sequence.PNA is useful in distinguishing between normal and tumor tissues and in assessing malignancy in transitional mucosa. In addition, PNA binding can be used to measure cellular maturity in lymphoid tissues, to distinguish a variety of lymphocyte subpopulations in man and experimental animals, and to measure the levels of lymphoid cell populations in many diseases. PNA can be employed in the fractionation of stem cells in mice for use in bone marrow transplantation across histocompatibility barriers.A major cell surface receptor for PNA may be asialo GM1 ganglioside. Since PNA shares specificity with the antibody to this glycolipid, PNA and the antibody can be used interchangeably in some applications.Agarose bound* PNA is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2×107 daltons are used as the solid-phase matrix to which the lectins are covalently coupled. The attachment of the lectins to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins that might result in nonspecific ionic or hydrophobic interactions. The technique we have developed to couple lectins to agarose beads inserts a hydrophilic spacer arm between the lectin and the matrix.This coupling method provides several advantages over the traditional cyanogen bromide procedure:Maximum carbohydrate binding activity of the coupled lectins is retainedLinkage is stable over a range of pH valuesConjugated proteins are not leached off the beads by Tris or other routinely used buffersNo residual charges are present after conjugation. This minimizes non-specific binding to the matrix.Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins.Inhibiting/Eluting Sugar: 200 mM galactose*5 mg lectin/ml gel
  • 反應種屬:
  • 宿主來源:
  • 實驗應用:
    Glycobiology,Affinity Chromatography
  • 靶標/特異性:
  • 同種型:
  • 推薦稀釋度:
    Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Use of buffers containing 0.1 mM CaCl2 and 0.01 mM MnCl2 is recommended. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-2100. Alternatively, 200 mM galactose in 10 mM HEPES-buffered saline, pH 7.5 can be used.For those glycoconjugates having very high affinity for PNA, it may be necessary to lower the pH of the eluting sugar solution to pH 3.0 with acetic acid. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
  • 免疫原:
  • 免疫原種屬:
  • 克隆性:
  • 克隆號:
  • 純化方式:
  • 偶聯:
    Agarose
  • 產品濃度:
  • 表達系統:
  • 陽性對照:
  • 保存溫度:
  • 運輸溫度:
  • 細胞定位:
  • 預期分子量:
  • 產品形式:
  • 存儲溶液:
    10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 20 mM galactose, 0.08% sodium azide
  • 有效期:
  • 產地:
    美國
  • 外觀:
  • 生產商:
  • 功能與背景:
  • URL:
  • 靈敏度:
  • 檢測范圍:
  • 檢測方法:
  • 樣本類型:
    10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 20 mM galactose, 0.08% sodium azide
  • 產品特點:
  • 純度:
  • 生物活性:
  • 內毒素水平:
  • 來源:
  • 表達方式:
  • Accession #:
  • CAS號:
  • 分子式:
  • 溶解度:
  • PubChem CID:

Vectorlabs 山羊抗大鼠IgG-Goat Anti-Rat IgG, Mouse Adsorbed, Antibody (H+L), Peroxidase, 0.5 mg

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Vectorlabs 山羊抗大鼠IgG-Goat Anti-Rat IgG, Mouse Adsorbed, Antibody (H+L), Peroxidase, 0.5 mg

Vector Lab代理
上海金畔生物代理Vector Lab品牌產品，歡迎訪問Vector Labs官網或者咨詢我們獲取相關產品信息。
Vector Laboratories主要產品類別
ABC免疫組化試劑盒、ImmPRESS聚合物免疫組化試劑盒、防熒光淬滅封片劑、二抗、酶底物、親和素/鏈霉親和素-酶結合物、封閉試劑。Goat Anti-Rat IgG, Mouse Adsorbed, Antibody (H+L), Peroxidase, 0.5 mg

品牌：Vectorlabs
產品貨號：PI-9401-0.5
規格：0.5 mg
產品詳情
* 以下信息僅供參考，詳情請以原廠網站為準
  • 產品名稱:
  • Goat Anti-Rat IgG, Mouse Adsorbed, Antibody (H+L), Peroxidase, 0.5 mg
  • 別名:
    山羊抗大鼠IgG（H+L）抗體，HRP標記
  • 產品描述:
  • Goat Anti-Rat IgG Antibody is purified by affinity chromatography and adsorbed to remove any cross-reactivities that are likely to interfere with specific labeling. This antibody is then cross-linked with the highest specific activity horseradish peroxidase. A proprietary procedure for conjugation allows the maximum preservation of enzyme activity and antibody specificity. This antibody is subjected to rigorous quality control assays and can be used for tissue staining, ELISAs, and blots.This specially prepared antibody is designed to be used on tissues that may contain interfering endogenous immunoglobulins. The anti-rat IgG (H+L), mouse adsorbed products have been thoroughly adsorbed by a solid phase technique to remove antibodies that cross-react with mouse immunoglobulins. This adsorbed antibody allows primary antibodies made in rat to be used on mouse tissues.Peroxidase Goat Anti-Rat IgG, mouse adsorbed, is supplied in solution. With some exceptions, working concentration of HRP Anti-Rat IgG, mouse adsorbed, is 0.5-5.0 ug/ml.
  • Goat Anti-Rat IgG Antibody 通過親和層析純化并吸附以去除任何可能干擾特異性標記的交叉反應。然后該抗體與最高比活性的辣根過氧化物酶交聯。專有的偶聯程序可最大限度地保留酶活性和抗體特異性。該抗體經過嚴格的質量控制檢測，可用于組織染色、ELISA 和印跡。這種特殊制備的抗體設計用于可能含有干擾性內源性免疫球蛋白的組織?？勾笫?IgG (H+L)、小鼠吸附產品已通過固相技術徹底吸附，以去除與小鼠免疫球蛋白交叉反應的抗體。這種吸附的抗體允許在大鼠中制備的一抗用于小鼠組織。過氧化物酶山羊抗大鼠 IgG，小鼠吸附，以溶液形式提供。除了一些例外，小鼠吸附的 HRP Anti-Rat IgG 的工作濃度為 0.5-5.0 ug/ml。
  • 反應種屬:
    Rat
  • 宿主來源:
    Goat
  • 實驗應用:
    Immunohistochemistry / Immunocytochemistry,Blotting Applications,Elispot,ELISAs
  • 推薦稀釋度:
  • Recommended diluent for microtitration plate assays: 10 mM phosphate, 0.15 M NaCl, pH 7.5, 0.1% Tween 20.Working concentration of HRP-anti rat IgG is 0.5-5.0μg/ml.
  • 推薦用于微量滴定板測定的稀釋劑：10 mM 磷酸鹽，0.15 M NaCl，pH 7.5，0.1% Tween 20。HRP-抗大鼠 IgG 的工作濃度為 0.5-5.0μg/ml。
  • 產品形式:
    Concentrate
  • 存儲溶液:
    10 mM HEPES, 0.15 M NaCl, pH 7.5, preservative
  • 樣本類型:
    10 mM HEPES, 0.15 M NaCl, pH 7.5, preservative

Vectorlabs Cy3標記接骨木凝集素 Sambucus Nigra Lectin (SNA, EBL), CY3, 1 mg

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Vectorlabs Cy3標記接骨木凝集素 Sambucus Nigra Lectin (SNA, EBL), CY3, 1 mg

Vector Lab代理
上海金畔生物代理Vector Lab品牌產品，歡迎訪問Vector Labs官網或者咨詢我們獲取相關產品信息。
Vector Laboratories主要產品類別
ABC免疫組化試劑盒、ImmPRESS聚合物免疫組化試劑盒、防熒光淬滅封片劑、二抗、酶底物、親和素/鏈霉親和素-酶結合物、封閉試劑。

Sambucus Nigra Lectin (SNA, EBL), CY?3, 1 mg

品牌：Vectorlabs
產品貨號：CL-1303-1
規格：1 mg
產品詳情
* 以下信息僅供參考，詳情請以原廠網站為準
  • 產品名稱:
    Sambucus Nigra Lectin (SNA, EBL), CY?3, 1 mg
  • 別名:
    接骨木凝集素，Cy3標記
  • 產品描述:
  • Cy3 Sambucus nigra lectin, SNL isolated from elderberry bark, binds preferentially to sialic acid attached to terminal galactose in α-2,6 and to a lesser degree, α-2,3 linkage. Binding is also inhibited to some extent by lactose or galactose. This lectin does not appear to bind sialic acid linked to N-acetylgalactosamine. SNA has been reported to inhibit cell-free protein synthesis.Cy3 labeled Sambucus nigra lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. Excitation: 550 nmEmission: 570 nmColor: Red
  • Cy3 Sambucus nigra 凝集素，從接骨木樹皮中分離出來的 SNL，優先與 α-2,6 末端半乳糖連接的唾液酸結合，而α-2,3 鍵的結合程度較低。 乳糖或半乳糖也在一定程度上抑制了結合。 這種凝集素似乎不結合與 N-乙酰半乳糖胺相連的唾液酸。 據報道，SNA 可抑制無細胞蛋白質合成。Cy3 標記的黑接骨木凝集素具有適當數量的熒光染料，可以為這種凝集素提供最佳染色特性。 該偶聯物基本上不含未偶聯的熒光染料。 激發：550 納米發射：570 納米顏色：紅色
  • 實驗應用:
    Immunofluorescence,Glycobiology
  • 推薦稀釋度:
    The recommended concentration range for use is 5-20μg/ml.
  • 偶聯:
    Cy3
  • 產品濃度:
    1 mg active conjugate/ml
  • 存儲溶液:
    10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, and a proprietary stabilizer.
  • 樣本類型:
    10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, and a proprietary stabilizer.

Vectorlabs 親和素/生物素封閉試劑盒 Avidin/Biotin Blocking Kit

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Vectorlabs 親和素/生物素封閉試劑盒 Avidin/Biotin Blocking Kit

Vector Lab代理
上海金畔生物代理Vector Lab品牌產品，歡迎訪問Vector Labs官網或者咨詢我們獲取相關產品信息。
Vector Laboratories主要產品類別
ABC免疫組化試劑盒、ImmPRESS聚合物免疫組化試劑盒、防熒光淬滅封片劑、二抗、酶底物、親和素/鏈霉親和素-酶結合物、封閉試劑。

Avidin/Biotin Blocking Kit, 1 Kit

品牌：Vectorlabs
產品貨號：SP-2001
規格：1 Kit
產品詳情
* 以下信息僅供參考，詳情請以原廠網站為準
  • 產品名稱:
    Avidin/Biotin Blocking Kit, 1 Kit
  • 別名:
    親和素/生物素封閉試劑盒
  • 產品描述:
  • Avidin Biotin Blocking Kit blocks all endogenous biotin, biotin receptors, and avidin binding sites present in tissues. This kit is designed for use with avidin detection systems such as the VECTASTAIN? ABC Kits if avidin or biotinylated reagents bind non-specifically to tissues or proteins.This blocking kit consists of 18 ml of Avidin D and 18 ml of biotin in convenient dropper bottles.
  • Avidin Biotin Blocking Kit 可阻斷組織中存在的所有內源性生物素、生物素受體和親和素結合位點。 如果抗生物素蛋白或生物素化試劑與組織或蛋白質非特異性結合，則該試劑盒設計用于抗生物素蛋白檢測系統，例如 VECTASTAIN? ABC 試劑盒。該封閉試劑盒由 18 毫升 Avidin D 和 18 毫升生物素組成，裝在方便的滴管瓶中 .
  • 實驗應用:
    Immunohistochemistry / Immunocytochemistry,Immunofluorescence,In situ hybridization,Blotting Applications,Elispot

Vectorlabs 核固紅復染試劑 Nuclear Fast Red Counterstain, 500 ml

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Vectorlabs 核固紅復染試劑 Nuclear Fast Red Counterstain, 500 ml

Vector Lab代理
上海金畔生物代理Vector Lab品牌產品，歡迎訪問Vector Labs官網或者咨詢我們獲取相關產品信息。
Vector Laboratories主要產品類別
ABC免疫組化試劑盒、ImmPRESS聚合物免疫組化試劑盒、防熒光淬滅封片劑、二抗、酶底物、親和素/鏈霉親和素-酶結合物、封閉試劑。

Nuclear Fast Red Counterstain, 500 ml

品牌：Vectorlabs
產品貨號：H-3403-500
規格：500 ml
產品詳情
* 以下信息僅供參考，詳情請以原廠網站為準
  • 產品名稱:
    Nuclear Fast Red Counterstain, 500 ml
  • 別名:
    核固紅復染試劑
  • 產品描述:
  • Vector’s Nuclear Fast Red counterstain stained nuclei have a pink appearance. This counterstain provides good contrast with a variety of substrates and is suitable for use with nonaqueous mountants. The staining procedure is rapid requiring only one step. This counterstain provides an excellent alternative in multiple labeling when hematoxylin obscures the substrate colors.
  • 實驗應用:
    Immunohistochemistry / Immunocytochemistry,In situ hybridization
  • 產品形式:
    Ready-to-Use
  • 有效期:
    效期最長8個月

Vectorlabs 馬抗山羊IgG Horse Anti-Goat IgG Antibody (H+L), Peroxidase, 1 mg

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Vectorlabs 馬抗山羊IgG Horse Anti-Goat IgG Antibody (H+L), Peroxidase, 1 mg

Vector Lab代理
上海金畔生物代理Vector Lab品牌產品，歡迎訪問Vector Labs官網或者咨詢我們獲取相關產品信息。
Vector Laboratories主要產品類別
ABC免疫組化試劑盒、ImmPRESS聚合物免疫組化試劑盒、防熒光淬滅封片劑、二抗、酶底物、親和素/鏈霉親和素-酶結合物、封閉試劑。

Horse Anti-Goat IgG Antibody (H+L), Peroxidase, 1 mg

品牌：Vectorlabs
產品貨號：PI-9500-1
規格：1 mg
產品詳情
* 以下信息僅供參考，詳情請以原廠網站為準
  • 產品名稱:
    Horse Anti-Goat IgG Antibody (H+L), Peroxidase, 1 mg
  • 別名:
    馬抗山羊IgG（H+L）抗體，HRP標記
  • 產品描述:
  • Horse Anti-Goat IgG Antibody is purified by affinity chromatography and adsorbed to remove any cross-reactivities that are likely to interfere with specific labeling. This antibody is then cross-linked with the highest specific activity horseradish peroxidase. A proprietary procedure for conjugation allows the maximum preservation of enzyme activity and antibody specificity. This antibody is subjected to rigorous quality control assays and can be used for tissue staining, ELISAs, and blots.As well as recognising goat IgG, this antibody is suitable to detect sheep and bovine IgG primary antibodies.
  • Horse Anti-Goat IgG Antibody 通過親和層析純化并吸附以去除任何可能干擾特定標記的交叉反應性。 然后該抗體與最高比活性的辣根過氧化物酶交聯。 專有的偶聯程序可最大限度地保留酶活性和抗體特異性。 該抗體經過嚴格的質量控制檢測，可用于組織染色、ELISA 和印跡。除識別山羊 IgG 外，該抗體還適用于檢測綿羊和牛 IgG 一抗。
  • 反應種屬:
    Goat
  • 宿主來源:
    Horse
  • 實驗應用:
    Immunohistochemistry / Immunocytochemistry,In situ hybridization,Blotting Applications,Elispot,ELISAs
  • 推薦稀釋度:
  • Important Note:The use of bovine products such as casein, serum, albumin or non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of anti-goat IgG with bovine immunoglobulins that may be present.If this antibody is to be used in thse applications, blocking with Animal-Free blocker??￠ solution (Cat. No. SP-5030) is recommended. Recommended diluent for microtitration plate assays: 10 mM phosphate, 0.15 M NaCl, pH 7.5, 0.1% Tween 20.Working concentration of HRP-anti goat IgG is 0.5-5.0μg/ml.
  • 重要提示：在 ELISA 或印跡分析中使用牛產品（如酪蛋白、血清、白蛋白或脫脂奶粉）作為封閉劑可能會產生高背景，因為抗山羊 IgG 與牛免疫球蛋白可能會產生交叉反應。 如果要在這些應用中使用該抗體，建議使用無動物阻滯劑，?￠ 溶液（貨號 SP-5030）進行封閉。 推薦用于微量滴定板測定的稀釋劑：10 mM 磷酸鹽、0.15 M NaCl、pH 7.5、0.1% Tween 20。HRP-抗山羊 IgG 的工作濃度為 0.5-5.0μg/ml。
  • 偶聯:
    Peroxidase
  • 產品濃度:
    1 mg active conjugate/ml
  • 產品形式:
    Concentrate
  • 存儲溶液:
    10 mM HEPES, 0.15 M NaCl, pH 7.5, preservative
  • 樣本類型:
    10 mM HEPES, 0.15 M NaCl, pH 7.5, preservative

Vectorlabs 瓊脂糖交聯花生凝集素 Peanut Agglutinin (PNA), Agarose bound, 5 ml

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Vectorlabs 瓊脂糖交聯花生凝集素 Peanut Agglutinin (PNA), Agarose bound, 5 ml

Vector Lab代理
上海金畔生物代理Vector Lab品牌產品，歡迎訪問Vector Labs官網或者咨詢我們獲取相關產品信息。
Vector Laboratories主要產品類別
ABC免疫組化試劑盒、ImmPRESS聚合物免疫組化試劑盒、防熒光淬滅封片劑、二抗、酶底物、親和素/鏈霉親和素-酶結合物、封閉試劑。

Peanut Agglutinin (PNA), Agarose bound, 5 ml

品牌：Vectorlabs
產品貨號：AL-1073-5
規格：5 ml
產品詳情
* 以下信息僅供參考，詳情請以原廠網站為準
  • 產品名稱:
    Peanut Agglutinin (PNA), Agarose bound, 5 ml
  • 別名:
    花生凝集素，瓊脂糖交聯
  • 產品描述:
  • Peanut agglutinin (PNA) binds preferentially to the T-antigen, a galactosyl (β-1,3) N-acetylgalactosamine structure present in many glycoconjugates such as M and N blood groups, gangliosides, and many other soluble and membrane-associated glycoproteins and glycolipids. With certain exceptions, the receptor sequence for PNA is normally sialylated which prevents the lectin from binding to its receptor oligosaccharide (see Jacalin). Even sialic acid which is not bound directly to the receptor sugars may inhibit binding. The presence of calcium ions in diluents can enhance the binding of PNA to receptors, possibly by neutralizing the negative charges on sialic acid residues adjacent to the receptor sequence.PNA is useful in distinguishing between normal and tumor tissues and in assessing malignancy in transitional mucosa. In addition, PNA binding can be used to measure cellular maturity in lymphoid tissues, to distinguish a variety of lymphocyte subpopulations in man and experimental animals, and to measure the levels of lymphoid cell populations in many diseases. PNA can be employed in the fractionation of stem cells in mice for use in bone marrow transplantation across histocompatibility barriers.A major cell surface receptor for PNA may be asialo GM1 ganglioside. Since PNA shares specificity with the antibody to this glycolipid, PNA and the antibody can be used interchangeably in some applications.Agarose bound* PNA is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2×107 daltons are used as the solid-phase matrix to which the lectins are covalently coupled. The attachment of the lectins to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins that might result in nonspecific ionic or hydrophobic interactions. The technique we have developed to couple lectins to agarose beads inserts a hydrophilic spacer arm between the lectin and the matrix.This coupling method provides several advantages over the traditional cyanogen bromide procedure:Maximum carbohydrate binding activity of the coupled lectins is retainedLinkage is stable over a range of pH valuesConjugated proteins are not leached off the beads by Tris or other routinely used buffersNo residual charges are present after conjugation. This minimizes non-specific binding to the matrix.Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins.Inhibiting/Eluting Sugar: 200 mM galactose*5 mg lectin/ml gel
  • 花生凝集素 (PNA) 優先與 T 抗原結合，這是一種半乳糖基 (β-1,3) N-乙酰半乳糖胺結構，存在于許多糖綴合物中，例如 M 和 N 血型、神經節苷脂和許多其他可溶性和膜相關糖蛋白和糖脂。除了某些例外，PNA 的受體序列通常是唾液酸化的，這會阻止凝集素與其受體寡糖結合（參見 Jacalin）。甚至不直接與受體糖結合的唾液酸也可能抑制結合。稀釋劑中鈣離子的存在可以增強 PNA 與受體的結合，可能通過中和受體序列附近唾液酸殘基上的負電荷。PNA 可用于區分正常組織和腫瘤組織以及評估移行黏膜的惡性腫瘤。此外，PNA 結合可用于測量淋巴組織中的細胞成熟度，區分人和實驗動物中的各種淋巴細胞亞群，以及測量許多疾病中淋巴細胞群的水平。 PNA 可用于分離小鼠干細胞，用于跨越組織相容性障礙的骨髓移植。PNA 的主要細胞表面受體可能是去唾液酸 GM1 神經節苷脂。由于 PNA 與針對這種糖脂的抗體具有特異性，因此 PNA 和抗體可以在某些應用中互換使用。瓊脂糖結合* PNA 是使用我們的親和純化凝集素制備的。具有約 2×107 道爾頓分子量排除極限的熱穩定、交聯的 4% 瓊脂糖珠用作凝集素共價偶聯的固相基質。凝集素與珠子的連接受到仔細控制，以保持凝集素活性并最大限度地減少可能導致非特異性離子或疏水相互作用的結合凝集素的構象變化。我們開發的將凝集素與瓊脂糖珠偶聯的技術在凝集素和基質之間插入了一個親水性間隔臂。這種偶聯方法與傳統的溴化氰方法相比具有幾個優點：保持偶聯凝集素的最大碳水化合物結合活性pH 值范圍 結合的蛋白質不會被 Tris 或其他常規使用的緩沖液從珠子上浸出 結合后不存在殘留電荷。這最大限度地減少了與基質的非特異性結合。我們的瓊脂糖結合凝集素以每毫升沉降珠子的恒定凝集素濃度提供。選擇每種凝集素的濃度以實現珠中每毫克凝集素的最高糖綴合物結合能力。使用已知結合凝集素的糖蛋白測試每批的結合能力。這為用戶提供了指導并確保了我們瓊脂糖結合凝集素的質量。抑制/洗脫糖：200 mM 半乳糖*5 mg 凝集素/ml 凝膠
  • 實驗應用:
    Glycobiology,Affinity Chromatography
  • 推薦稀釋度:
  • Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Use of buffers containing 0.1 mM CaCl2 and 0.01 mM MnCl2 is recommended. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-2100. Alternatively, 200 mM galactose in 10 mM HEPES-buffered saline, pH 7.5 can be used.For those glycoconjugates having very high affinity for PNA, it may be necessary to lower the pH of the eluting sugar solution to pH 3.0 with acetic acid. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
  • 使用前用緩沖液徹底清洗凝膠，以去除為穩定凝集素而添加的糖。 建議使用含有 0.1 mM CaCl2 和 0.01 mM MnCl2 的緩沖液。 用于洗脫與這種瓊脂糖凝集素結合的糖綴合物的推薦產品：糖蛋白洗脫溶液，目錄 編號 ES-2100。 或者，可以使用 10 mM HEPES 緩沖鹽水中的 200 mM 半乳糖，pH 7.5。對于那些對 PNA 具有非常高親和力的糖綴合物，可能需要用乙酸將洗脫糖溶液的 pH 值降低到 3.0。 使用后，用幾柱體積的緩沖鹽水清洗凝膠，然后將凝膠重懸于含有 0.08% 疊氮化鈉的緩沖鹽水中保存。
  • 存儲溶液:
  • 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2,20 mM galactose, 0.08% sodium azide
  • 樣本類型:
  • 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2,20 mM
  • galactose, 0.08% sodium azide

Vectorlabs 鏈霉親和素/生物素封閉試劑盒 Streptavidin/Biotin Blocking Kit

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Vectorlabs 鏈霉親和素/生物素封閉試劑盒 Streptavidin/Biotin Blocking Kit

Vector Lab代理
上海金畔生物代理Vector Lab品牌產品，歡迎訪問Vector Labs官網或者咨詢我們獲取相關產品信息。
Vector Laboratories主要產品類別
ABC免疫組化試劑盒、ImmPRESS聚合物免疫組化試劑盒、防熒光淬滅封片劑、二抗、酶底物、親和素/鏈霉親和素-酶結合物、封閉試劑。

Streptavidin/Biotin Blocking Kit, 1 kit

品牌：Vectorlabs
產品貨號：SP-2002
規格：1 kit
產品詳情
* 以下信息僅供參考，詳情請以原廠網站為準
  • 產品名稱:
    Streptavidin/Biotin Blocking Kit, 1 kit
  • 別名:
    鏈霉親和素/生物素封閉試劑盒
  • 產品描述:
  • Streptavidin Biotin Blocking Kit blocks all endogenous biotin, biotin receptors, and streptavidin binding sites present in tissues. This kit is designed for use with streptavidin detection systems such as the VECTASTAIN? Universal Quick kits if streptavidin or biotinylated reagents bind non-specifically to tissues or proteins.This blocking kit consists of 18 ml of streptavidin and 18 ml of biotin in convenient dropper bottles.
  • Streptavidin Biotin Blocking Kit 可阻斷組織中存在的所有內源性生物素、生物素受體和鏈霉親和素結合位點。 如果鏈霉親和素或生物素化試劑與組織或蛋白質非特異性結合，則該試劑盒設計用于鏈霉親和素檢測系統，例如 VECTASTAIN? Universal Quick 試劑盒。該封閉試劑盒由 18 毫升鏈霉親和素和 18 毫升生物素組成，裝在方便的滴管瓶中 .
  • 實驗應用:
    Immunohistochemistry / Immunocytochemistry,Immunofluorescence,In situ hybridization,Blotting Applications,Elispot
  • 產品形式:
    Ready-to-Use

Vectorlabs 蘇木精/蘇木素QS復染試劑 Hematoxylin QS Counterstain, 100 ml

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Vectorlabs 蘇木精/蘇木素QS復染試劑 Hematoxylin QS Counterstain, 100 ml

Vector Lab代理
上海金畔生物代理Vector Lab品牌產品，歡迎訪問Vector Labs官網或者咨詢我們獲取相關產品信息。
Vector Laboratories主要產品類別
ABC免疫組化試劑盒、ImmPRESS聚合物免疫組化試劑盒、防熒光淬滅封片劑、二抗、酶底物、親和素/鏈霉親和素-酶結合物、封閉試劑。

Hematoxylin QS Counterstain, 100 ml

品牌：Vectorlabs
產品貨號：H-3404-100
規格：100 ml
產品詳情
* 以下信息僅供參考，詳情請以原廠網站為準
  • 產品名稱:
    Hematoxylin QS Counterstain, 100 ml
  • 別名:
    蘇木精/蘇木素QS復染試劑
  • 產品描述:
  • Vector’s Hematoxylin QS counterstain, a modification of Mayer s hematoxylin developed especially for immunocytochemistry, provides crisp blue nuclear staining. This formula is ready-touse without filtration, requires no separate blueing step, and stains in less than 45 seconds. Vector Hematoxylin QS contains no mercury and provides excellent color contrast with most commonly used peroxidase and alkaline phosphatase substrates. This counterstain is suitable for use with nonaqueous and aqueous mountants.
  • Vector 的蘇木精 QS 復染劑是 Mayer 蘇木精的改良版，專為免疫細胞化學開發，可提供清晰的藍色核染色。 該配方無需過濾即可使用，無需單獨的上藍步驟，并且可在 45 秒內染色。 Vector Hematoxylin QS 不含汞，與最常用的過氧化物酶和堿性磷酸酶底物具有出色的顏色對比。 這種復染劑適用于非水性和水性封固劑。
  • 實驗應用:
    Immunohistochemistry / Immunocytochemistry,In situ hybridization
  • 產品形式:
    Ready-to-Use